BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0188700 Os12g0188700|AK061678
(172 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0188700 Similar to Thioredoxin (TRX) 293 4e-80
Os02g0639900 Similar to Thioredoxin M-type, chloroplast pre... 148 2e-36
Os04g0530600 Similar to Thioredoxin 1 (TRX-1) (Thioredoxin M) 142 2e-34
Os04g0430800 Thioredoxin family protein 119 1e-27
Os04g0676100 Similar to Thioredoxin X, chloroplast precursor 84 4e-17
Os01g0963400 Thioredoxin family protein 84 6e-17
U92541 72 2e-13
Os07g0186000 Similar to Thioredoxin h isoform 1 72 2e-13
Os05g0508500 Similar to Thioredoxin H 70 1e-12
Os01g0339900 Thioredoxin domain 2 containing protein 69 2e-12
Os08g0378900 Similar to Thioredoxin protein 67 8e-12
Os05g0156300 Similar to Protein disulfide isomerase 66 1e-11
Os01g0168200 Similar to Thioredoxin-like protein 66 2e-11
Os05g0480200 Thioredoxin domain 2 containing protein 64 4e-11
AK110198 63 8e-11
>Os12g0188700 Similar to Thioredoxin (TRX)
Length = 172
Score = 293 bits (751), Expect = 4e-80, Method: Compositional matrix adjust.
Identities = 155/172 (90%), Positives = 155/172 (90%)
Query: 1 MALETCFRAWATLHAPQPPXXXXXXXXXXXXXXXXXQSKPRLSVASPSPLRPASRFACQC 60
MALETCFRAWATLHAPQPP QSKPRLSVASPSPLRPASRFACQC
Sbjct: 1 MALETCFRAWATLHAPQPPSSGGSRDRLLLSGAGSSQSKPRLSVASPSPLRPASRFACQC 60
Query: 61 SNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCK 120
SNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCK
Sbjct: 61 SNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCK 120
Query: 121 VNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATIIDKYVSS 172
VNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATIIDKYVSS
Sbjct: 121 VNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATIIDKYVSS 172
>Os02g0639900 Similar to Thioredoxin M-type, chloroplast precursor (TRX-M)
Length = 173
Score = 148 bits (373), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 64/100 (64%), Positives = 85/100 (85%)
Query: 72 EKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIAT 131
+ W S+V+ SE PVLVEFWA WCGPC+MI PVI +L+KEY GK+ C K+NTD++P+IAT
Sbjct: 73 KSTWQSLVVESELPVLVEFWASWCGPCKMIDPVIGKLSKEYEGKLNCYKLNTDENPDIAT 132
Query: 132 NYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATIIDKYVS 171
+GIRSIPT+++FKNGEKK++VIGAVP++TL + IDKY+
Sbjct: 133 QFGIRSIPTMMIFKNGEKKDAVIGAVPESTLVSSIDKYIG 172
>Os04g0530600 Similar to Thioredoxin 1 (TRX-1) (Thioredoxin M)
Length = 180
Score = 142 bits (357), Expect = 2e-34, Method: Compositional matrix adjust.
Identities = 63/114 (55%), Positives = 89/114 (78%)
Query: 57 ACQCSNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKI 116
A Q + +V + W S+V+ SE PVLV +WA WCGPC+MI PV+ +L+KEY GK+
Sbjct: 65 AVQGQDTSIQVPEVTKSTWQSLVMESELPVLVGYWATWCGPCKMIDPVVGKLSKEYEGKL 124
Query: 117 KCCKVNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATIIDKYV 170
KC K+NTD++P+IA+ YG+RSIPT+++FKNGEKK++VIGAVP++TL I+K+V
Sbjct: 125 KCYKLNTDENPDIASQYGVRSIPTMMIFKNGEKKDAVIGAVPESTLIASIEKFV 178
>Os04g0430800 Thioredoxin family protein
Length = 266
Score = 119 bits (297), Expect = 1e-27, Method: Compositional matrix adjust.
Identities = 46/79 (58%), Positives = 62/79 (78%)
Query: 81 GSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPT 140
+ PVL+EFWA WCGPCRM+ ++DE+A+EY G+IKC K++TDD P +AT+Y I IPT
Sbjct: 31 AGDIPVLIEFWASWCGPCRMVHRIVDEIAQEYAGRIKCYKLDTDDYPQVATSYSIERIPT 90
Query: 141 VLMFKNGEKKESVIGAVPK 159
VL+FK+GEK S+ G +PK
Sbjct: 91 VLLFKDGEKTHSITGTLPK 109
>Os04g0676100 Similar to Thioredoxin X, chloroplast precursor
Length = 180
Score = 84.3 bits (207), Expect = 4e-17, Method: Compositional matrix adjust.
Identities = 37/103 (35%), Positives = 66/103 (64%), Gaps = 2/103 (1%)
Query: 72 EKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIAT 131
+ +++ VL S+ PVLV+F A WCGPCR+IAPV+D A+EY G++K K++ D +P +
Sbjct: 74 QSEFEAEVLQSDLPVLVDFVADWCGPCRLIAPVVDWAAEEYEGRLKIVKIDHDANPQLIE 133
Query: 132 NYGIRSIPTVLMFKNGEK--KESVIGAVPKTTLATIIDKYVSS 172
Y + +P++++FK+G++ GA+ K ++ +S+
Sbjct: 134 EYKVYGLPSLILFKDGKEVPGSRREGAITKAKFKEYLEPLLST 176
>Os01g0963400 Thioredoxin family protein
Length = 122
Score = 84.0 bits (206), Expect = 6e-17, Method: Compositional matrix adjust.
Identities = 35/94 (37%), Positives = 61/94 (64%)
Query: 74 NWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNY 133
++D ++ SE PVLV+F+A WCGPC+ + P++ E++++ KI+ K++T+ +IA Y
Sbjct: 22 SFDELLEKSEKPVLVDFYATWCGPCQYMVPILQEVSEKLGDKIQVVKIDTEKYTSIANRY 81
Query: 134 GIRSIPTVLMFKNGEKKESVIGAVPKTTLATIID 167
I ++PT ++FKNG+ GA+P L I+
Sbjct: 82 QIEALPTFIIFKNGKPCHRFEGALPANQLIQQIE 115
>U92541
Length = 122
Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 35/87 (40%), Positives = 53/87 (60%), Gaps = 2/87 (2%)
Query: 86 VLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMFK 145
V+++F A WCGPCR IAPV E AK++ G + KV+ D+ +A Y + ++PT L K
Sbjct: 31 VIIDFTASWCGPCRFIAPVFAEYAKKFPGAV-FLKVDVDELKEVAEKYNVEAMPTFLFIK 89
Query: 146 NGEKKESVIGAVPKTTLATIIDKYVSS 172
+G + + V+GA K L I K+V +
Sbjct: 90 DGAEADKVVGA-RKDDLQNTIVKHVGA 115
>Os07g0186000 Similar to Thioredoxin h isoform 1
Length = 122
Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 35/87 (40%), Positives = 53/87 (60%), Gaps = 2/87 (2%)
Query: 86 VLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMFK 145
V+++F A WCGPCR IAPV E AK++ G + KV+ D+ +A Y + ++PT L K
Sbjct: 31 VIIDFTASWCGPCRFIAPVFAEYAKKFPGAV-FLKVDVDELKEVAEKYNVEAMPTFLFIK 89
Query: 146 NGEKKESVIGAVPKTTLATIIDKYVSS 172
+G + + V+GA K L I K+V +
Sbjct: 90 DGAEADKVVGA-RKDDLQNTIVKHVGA 115
>Os05g0508500 Similar to Thioredoxin H
Length = 121
Score = 69.7 bits (169), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 33/86 (38%), Positives = 57/86 (66%), Gaps = 2/86 (2%)
Query: 86 VLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMFK 145
V+++F A WCGPCR+IAPV +LAK++ + KV+ D+ IA + + ++PT L K
Sbjct: 37 VVIDFTASWCGPCRIIAPVFADLAKKHTNAV-FLKVDVDELKPIAEQFSVEAMPTFLFMK 95
Query: 146 NGEKKESVIGAVPKTTLATIIDKYVS 171
G+ K+ V+GA+ K LA+ ++ +++
Sbjct: 96 EGDVKDRVVGAM-KDELASKLELHMA 120
>Os01g0339900 Thioredoxin domain 2 containing protein
Length = 371
Score = 68.6 bits (166), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 32/85 (37%), Positives = 48/85 (56%), Gaps = 2/85 (2%)
Query: 67 VVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYV--GKIKCCKVNTD 124
VVV N+DS+VL +LVEF+APWCG C+ +AP+ ++LA Y + ++ D
Sbjct: 155 VVVLGPDNFDSIVLDENKDILVEFYAPWCGHCKHLAPIYEKLASVYKLDDGVVIANLDAD 214
Query: 125 DSPNIATNYGIRSIPTVLMFKNGEK 149
++A YG+ PT+ F G K
Sbjct: 215 KHKDLAEKYGVSGYPTLKFFPKGNK 239
>Os08g0378900 Similar to Thioredoxin protein
Length = 189
Score = 66.6 bits (161), Expect = 8e-12, Method: Compositional matrix adjust.
Identities = 34/98 (34%), Positives = 56/98 (57%), Gaps = 3/98 (3%)
Query: 73 KNWDSMVLGS-EAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIAT 131
K + +V G + P++V+F+A WCGPC ++A I+ LA EY KV+TDD +A
Sbjct: 89 KELEELVRGERKVPLIVDFYATWCGPCVLMAQDIEMLAVEYENNALFVKVDTDDEYELAR 148
Query: 132 NYGIRSIPTVLMFKNGEKKESVI--GAVPKTTLATIID 167
+ +R +PT+ F + K+++ G +P + IID
Sbjct: 149 DMQVRGLPTLYFFSPDQSKDALRTEGLIPIDMIRNIID 186
>Os05g0156300 Similar to Protein disulfide isomerase
Length = 366
Score = 65.9 bits (159), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 32/85 (37%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
Query: 67 VVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGK--IKCCKVNTD 124
VVV + +DS+VL VLVEF+APWCG C+ +AP+ ++LA Y + ++ D
Sbjct: 150 VVVLTPETFDSVVLDETKDVLVEFYAPWCGHCKHLAPIYEKLASVYKQDEGVVIANLDAD 209
Query: 125 DSPNIATNYGIRSIPTVLMFKNGEK 149
+A YG+ PT+ F G K
Sbjct: 210 KHTALAEKYGVSGFPTLKFFPKGNK 234
>Os01g0168200 Similar to Thioredoxin-like protein
Length = 131
Score = 65.9 bits (159), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 30/92 (32%), Positives = 56/92 (60%), Gaps = 4/92 (4%)
Query: 68 VVADEKNWDSMVLGSEAP---VLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTD 124
V+++++NWD + + V+ F A WCGPCR+IAPV E+++ Y + ++ D
Sbjct: 25 VISNKENWDHKIAEANKDGKIVIANFSAAWCGPCRVIAPVYAEMSQTYP-QFMFLTIDVD 83
Query: 125 DSPNIATNYGIRSIPTVLMFKNGEKKESVIGA 156
+ + ++++ IR+ PT KNGE+ + ++GA
Sbjct: 84 ELMDFSSSWDIRATPTFFFLKNGEQVDKLVGA 115
>Os05g0480200 Thioredoxin domain 2 containing protein
Length = 135
Score = 64.3 bits (155), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 33/92 (35%), Positives = 52/92 (56%), Gaps = 4/92 (4%)
Query: 68 VVADEKNWDSM---VLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVNTD 124
+V D+KNWD+ V V+++F A WC PCR AP+ EL+ +Y I V+ D
Sbjct: 24 LVTDQKNWDNTMEEVAEHGKTVVLKFSAIWCTPCRNAAPLFAELSLKY-PDIVFVSVDVD 82
Query: 125 DSPNIATNYGIRSIPTVLMFKNGEKKESVIGA 156
+ P + T Y +R+ PT + KN E+ + ++G
Sbjct: 83 EMPELVTQYDVRATPTFIFMKNNEEIDKLVGG 114
>AK110198
Length = 961
Score = 63.2 bits (152), Expect = 8e-11, Method: Composition-based stats.
Identities = 37/103 (35%), Positives = 57/103 (55%), Gaps = 2/103 (1%)
Query: 63 VVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRMIAPVIDELAKEYVGKIKCCKVN 122
+V EVV A E + + GS+ V+V+F A WCGPC++IAPV +L + + KV+
Sbjct: 1 MVKEVVSATEFDAELQAAGSKL-VVVDFHALWCGPCKVIAPVFQQLVTRH-PNVTFLKVD 58
Query: 123 TDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKTTLATI 165
D +A Y +R++PT L KN +++ GA P A +
Sbjct: 59 VDRVQAVAQRYRVRAMPTFLFLKNKNVVDTLQGADPNRLTALV 101
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.132 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,728,597
Number of extensions: 223569
Number of successful extensions: 510
Number of sequences better than 1.0e-10: 16
Number of HSP's gapped: 512
Number of HSP's successfully gapped: 16
Length of query: 172
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 78
Effective length of database: 12,127,685
Effective search space: 945959430
Effective search space used: 945959430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 152 (63.2 bits)