BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0562600 Os02g0562600|AK121163
(82 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0562600 Mlo-related protein family protein 160 1e-40
Os01g0888600 Similar to Seven transmembrane protein Mlo5 (F... 119 5e-28
Os05g0418100 Similar to Seven transmembrane protein Mlo8 111 1e-25
Os04g0444400 Mlo-related protein family protein 106 3e-24
Os03g0129100 Seven transmembrane protein MLO2 82 7e-17
Os10g0541000 Similar to Seven transmembrane protein Mlo2 81 2e-16
Os06g0610500 Similar to Seven transmembrane protein Mlo7 78 1e-15
AF388195 77 3e-15
Os05g0183300 Similar to Seven transmembrane protein Mlo6 76 5e-15
Os02g0197200 Mlo-related protein family protein 74 2e-14
Os04g0680800 Mlo-related protein family protein 65 1e-11
Os06g0486300 Similar to Mlo 63 6e-11
>Os02g0562600 Mlo-related protein family protein
Length = 82
Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust.
Identities = 82/82 (100%), Positives = 82/82 (100%)
Query: 1 TLDLFFFQMLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLI 60
TLDLFFFQMLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLI
Sbjct: 1 TLDLFFFQMLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLI 60
Query: 61 HIVLFQNAFEIAFFFWLLVRTT 82
HIVLFQNAFEIAFFFWLLVRTT
Sbjct: 61 HIVLFQNAFEIAFFFWLLVRTT 82
>Os01g0888600 Similar to Seven transmembrane protein Mlo5 (Fragment)
Length = 502
Score = 119 bits (298), Expect = 5e-28, Method: Compositional matrix adjust.
Identities = 54/71 (76%), Positives = 65/71 (91%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
+LL VGTKLEH+IT+LAH+VAEKH+AIEG+LVV+PSDE FWF RPK++L LIH +LFQNA
Sbjct: 313 LLLAVGTKLEHVITQLAHEVAEKHSAIEGDLVVNPSDEHFWFGRPKVILYLIHFILFQNA 372
Query: 69 FEIAFFFWLLV 79
FEIAFFFW+L
Sbjct: 373 FEIAFFFWILT 383
>Os05g0418100 Similar to Seven transmembrane protein Mlo8
Length = 287
Score = 111 bits (277), Expect = 1e-25, Method: Compositional matrix adjust.
Identities = 50/70 (71%), Positives = 61/70 (87%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
+LL VGTKLEH+I +LAH VAEK++AIEG+LVV PSD+ FW RP+I+L LIH +LFQNA
Sbjct: 97 LLLAVGTKLEHVIAQLAHDVAEKNSAIEGDLVVKPSDDHFWLGRPRIILYLIHFILFQNA 156
Query: 69 FEIAFFFWLL 78
FEIAFFFW+L
Sbjct: 157 FEIAFFFWIL 166
>Os04g0444400 Mlo-related protein family protein
Length = 460
Score = 106 bits (265), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 46/77 (59%), Positives = 62/77 (80%)
Query: 3 DLFFFQMLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHI 62
D ++LL++GTKLEHIIT+LA++VA KHTA+EG++ VSPSD LFWFH P++VL L+
Sbjct: 286 DFLCEKLLLLIGTKLEHIITQLAYEVATKHTAVEGDIAVSPSDNLFWFHSPRLVLALLRF 345
Query: 63 VLFQNAFEIAFFFWLLV 79
+LFQNAFE A+F W +
Sbjct: 346 ILFQNAFEFAYFIWTMA 362
>Os03g0129100 Seven transmembrane protein MLO2
Length = 555
Score = 82.4 bits (202), Expect = 7e-17, Method: Compositional matrix adjust.
Identities = 36/71 (50%), Positives = 53/71 (74%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
+L++VGTKLE +I E+A ++ ++ T I+G VV PS++ FWF+RP VL IH++LFQNA
Sbjct: 315 ILMLVGTKLEMVIMEMAQEIQDRATVIKGAPVVEPSNKYFWFNRPDWVLFFIHLILFQNA 374
Query: 69 FEIAFFFWLLV 79
F++A F W L
Sbjct: 375 FQMAHFVWTLA 385
>Os10g0541000 Similar to Seven transmembrane protein Mlo2
Length = 537
Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 41/80 (51%), Positives = 55/80 (68%), Gaps = 5/80 (6%)
Query: 3 DLFFFQML-----LVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVL 57
+LF+F + L VGTKL+ II +A ++ E+HT I+G VV SDE FWF +P++VL
Sbjct: 259 NLFWFSTIPLVVTLAVGTKLQAIIAMMAVEIKERHTVIQGMPVVKLSDEHFWFGKPRLVL 318
Query: 58 LLIHIVLFQNAFEIAFFFWL 77
LIH FQNAFEI +FFW+
Sbjct: 319 HLIHFASFQNAFEITYFFWI 338
>Os06g0610500 Similar to Seven transmembrane protein Mlo7
Length = 227
Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 42/71 (59%), Positives = 47/71 (66%), Gaps = 3/71 (4%)
Query: 12 VVGTKLEHIITELAHQ---VAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
VVG KLEHIIT LA + VA E V PS E FWF RP+IVL LIH +LFQN+
Sbjct: 23 VVGAKLEHIITRLAQEAAAVASLSHGTERTPYVKPSKEHFWFGRPEIVLNLIHFILFQNS 82
Query: 69 FEIAFFFWLLV 79
FEI FF W+LV
Sbjct: 83 FEIGFFIWVLV 93
>AF388195
Length = 554
Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 33/71 (46%), Positives = 51/71 (71%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
++L+VGTKLE +I ++A ++ ++ T I+G VV PS++ FWF+RP VL IH+ LF NA
Sbjct: 316 IVLLVGTKLEMVIMQMAQEIQDRATVIQGAPVVEPSNKYFWFNRPDWVLFFIHLTLFHNA 375
Query: 69 FEIAFFFWLLV 79
F++A F W +
Sbjct: 376 FQMAHFVWTMA 386
>Os05g0183300 Similar to Seven transmembrane protein Mlo6
Length = 311
Score = 76.3 bits (186), Expect = 5e-15, Method: Composition-based stats.
Identities = 35/69 (50%), Positives = 45/69 (65%)
Query: 10 LLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNAF 69
+L VGTKL+ II +A + E+H I+G +V SD FWF RP VL LIH LFQN F
Sbjct: 100 ILSVGTKLQGIICRMAIDITERHAVIQGIPLVQVSDSYFWFSRPTFVLFLIHFTLFQNGF 159
Query: 70 EIAFFFWLL 78
+I +F W+L
Sbjct: 160 QIIYFLWIL 168
>Os02g0197200 Mlo-related protein family protein
Length = 386
Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 35/68 (51%), Positives = 44/68 (64%)
Query: 12 VVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNAFEI 71
+VGTKLE I T LA + A+ G P E FWF +P+IVL LIH +LFQN+FE+
Sbjct: 278 IVGTKLELISTRLAQEAADCPDEATGNPWTKPCKEHFWFSKPRIVLHLIHFILFQNSFEM 337
Query: 72 AFFFWLLV 79
FFFW+L
Sbjct: 338 GFFFWVLA 345
>Os04g0680800 Mlo-related protein family protein
Length = 326
Score = 65.1 bits (157), Expect = 1e-11, Method: Composition-based stats.
Identities = 29/69 (42%), Positives = 46/69 (66%), Gaps = 3/69 (4%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLFQNA 68
++L+VG KL+++I L + A+ + + P D+LFWF +P+ +L LIH +LFQN+
Sbjct: 46 LVLLVGAKLQYVIATLTAEGAKMNAYGPR---IKPRDDLFWFKKPEFLLWLIHFILFQNS 102
Query: 69 FEIAFFFWL 77
FE+A FFW
Sbjct: 103 FELASFFWF 111
>Os06g0486300 Similar to Mlo
Length = 528
Score = 62.8 bits (151), Expect = 6e-11, Method: Compositional matrix adjust.
Identities = 27/57 (47%), Positives = 42/57 (73%)
Query: 9 MLLVVGTKLEHIITELAHQVAEKHTAIEGELVVSPSDELFWFHRPKIVLLLIHIVLF 65
++L+VGTKLE +I ++A ++ ++ T I+G VV PS++ FWF+RP VL IH+ LF
Sbjct: 302 IVLLVGTKLEMVIMQMAQEIQDRATVIQGAPVVEPSNKYFWFNRPDWVLFFIHLTLF 358
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.334 0.146 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,401,238
Number of extensions: 76209
Number of successful extensions: 224
Number of sequences better than 1.0e-10: 12
Number of HSP's gapped: 222
Number of HSP's successfully gapped: 12
Length of query: 82
Length of database: 17,035,801
Length adjustment: 52
Effective length of query: 30
Effective length of database: 14,320,673
Effective search space: 429620190
Effective search space used: 429620190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 149 (62.0 bits)