BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0136200 Os01g0136200|AK121025
(150 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0136200 16.9 kDa class I heat shock protein 240 2e-64
Os01g0136100 16.9 kDa class I heat shock protein 239 6e-64
Os01g0136000 Similar to Cytosolic class I small heat-shock ... 223 4e-59
Os03g0267000 Low molecular mass heat shock protein Oshsp18.0 179 9e-46
Os03g0266300 Class I low-molecular-weight heat shock protei... 177 2e-45
Os03g0266900 Low molecular mass heat shock protein Oshsp17.3 175 9e-45
Os03g0267200 Low molecular mass heat shock protein Oshsp17.7 168 1e-42
Os01g0135800 Similar to Cytosolic class I small heat shock ... 132 8e-32
Os02g0128000 Similar to Cytosolic class I small heat shock ... 96 1e-20
Os04g0445100 Similar to 22.7 kDa class IV heat shock protei... 80 4e-16
Os06g0253100 HSP20-like chaperone domain containing protein 69 1e-12
Os11g0244200 Similar to Pisum sativum 17.9 kDa heat shock p... 64 4e-11
Os02g0217900 Similar to Cytosolic class II small heat shock... 63 8e-11
>Os01g0136200 16.9 kDa class I heat shock protein
Length = 150
Score = 240 bits (613), Expect = 2e-64, Method: Compositional matrix adjust.
Identities = 122/150 (81%), Positives = 122/150 (81%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL 60
MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL
Sbjct: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL 60
Query: 61 PGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 120
PG GNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV
Sbjct: 61 PGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 120
Query: 121 KAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
KAGMENGVL IEISG
Sbjct: 121 KAGMENGVLTVTVPKAEVKKPEVKAIEISG 150
>Os01g0136100 16.9 kDa class I heat shock protein
Length = 150
Score = 239 bits (610), Expect = 6e-64, Method: Compositional matrix adjust.
Identities = 121/150 (80%), Positives = 122/150 (81%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL 60
MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL
Sbjct: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL 60
Query: 61 PGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 120
PG GNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV
Sbjct: 61 PGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 120
Query: 121 KAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
KAG+ENGVL IEISG
Sbjct: 121 KAGLENGVLTVTVPKAEVKKPEVKAIEISG 150
>Os01g0136000 Similar to Cytosolic class I small heat-shock protein HSP17.5
Length = 149
Score = 223 bits (568), Expect = 4e-59, Method: Compositional matrix adjust.
Identities = 114/150 (76%), Positives = 117/150 (78%), Gaps = 1/150 (0%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADL 60
MSLVRRSNVFDPF+ D WDPFD VFRS+VPATSD DTAAFANAR+DWKETPESHVFKADL
Sbjct: 1 MSLVRRSNVFDPFA-DFWDPFDGVFRSLVPATSDRDTAAFANARVDWKETPESHVFKADL 59
Query: 61 PGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 120
PG GNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV
Sbjct: 60 PGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQV 119
Query: 121 KAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
KA MENGVL IEISG
Sbjct: 120 KASMENGVLTVTVPKAEVKKPEVKAIEISG 149
>Os03g0267000 Low molecular mass heat shock protein Oshsp18.0
Length = 161
Score = 179 bits (453), Expect = 9e-46, Method: Compositional matrix adjust.
Identities = 92/162 (56%), Positives = 109/162 (67%), Gaps = 13/162 (8%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDS------------VFRSVVPATSDNDTAAFANARIDWK 48
MSL+RRSNVFDPFSLDLWDPFD +F S P + ++TAAFA ARIDWK
Sbjct: 1 MSLIRRSNVFDPFSLDLWDPFDGFPFGSGSRSSGSIFPSF-PRGTSSETAAFAGARIDWK 59
Query: 49 ETPESHVFKADLPGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRR 108
ETPE+HVFKAD+PG GNVL ISG+RSKE+E+K DKWHRVERSSG+F+RR
Sbjct: 60 ETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERSKEQEEKTDKWHRVERSSGKFLRR 119
Query: 109 FRLPENAKVDQVKAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
FRLPEN K +Q+KA MENGVL I+++G
Sbjct: 120 FRLPENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQVTG 161
>Os03g0266300 Class I low-molecular-weight heat shock protein 17.9
Length = 161
Score = 177 bits (449), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 89/161 (55%), Positives = 107/161 (66%), Gaps = 11/161 (6%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDSVFRSVV-----------PATSDNDTAAFANARIDWKE 49
MSL+RRSNVFDPFSLDLWDPFD P + ++TAAFA ARIDWKE
Sbjct: 1 MSLIRRSNVFDPFSLDLWDPFDGFPFGSGGSSSGSIFPSFPRGASSETAAFAGARIDWKE 60
Query: 50 TPESHVFKADLPGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRF 109
TPE+HVFKAD+PG GN+L ISG+R+KE+E+K D+WHRVERSSG+F+RRF
Sbjct: 61 TPEAHVFKADVPGLKKEEVKVEVDDGNILQISGERNKEQEEKTDQWHRVERSSGKFLRRF 120
Query: 110 RLPENAKVDQVKAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
RLP+NAK +Q+KA MENGVL I+ISG
Sbjct: 121 RLPDNAKPEQIKASMENGVLTVTVPKEEAKKPDVKSIQISG 161
>Os03g0266900 Low molecular mass heat shock protein Oshsp17.3
Length = 154
Score = 175 bits (444), Expect = 9e-45, Method: Compositional matrix adjust.
Identities = 91/155 (58%), Positives = 108/155 (69%), Gaps = 6/155 (3%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFD-----SVFRSVVPATSDNDTAAFANARIDWKETPESHV 55
MS++RRSNVFDPFSLDLWDPFD S S+ P +++D AAFA ARIDWKETPE+HV
Sbjct: 1 MSMIRRSNVFDPFSLDLWDPFDGFPFGSGSGSLFP-RANSDAAAFAGARIDWKETPEAHV 59
Query: 56 FKADLPGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENA 115
FKAD+PG GNVL ISG+R KE+E+K DKWHRVERSSG+F+RRFRLPEN
Sbjct: 60 FKADVPGLKKEEVKVEVEDGNVLQISGERIKEQEEKTDKWHRVERSSGKFLRRFRLPENT 119
Query: 116 KVDQVKAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
K +Q+KA MENGVL I+I+G
Sbjct: 120 KPEQIKASMENGVLTVTVPKEEPKKPDVKSIQITG 154
>Os03g0267200 Low molecular mass heat shock protein Oshsp17.7
Length = 159
Score = 168 bits (426), Expect = 1e-42, Method: Compositional matrix adjust.
Identities = 86/159 (54%), Positives = 103/159 (64%), Gaps = 9/159 (5%)
Query: 1 MSLVRRSNVFDPFSLDLWDPFDSV---------FRSVVPATSDNDTAAFANARIDWKETP 51
MSL+RR N FDPFSLDLWDP D + +++D AAFA ARIDWKETP
Sbjct: 1 MSLIRRGNAFDPFSLDLWDPVDGFPFGSGGSSSSSGSLFPRANSDAAAFAGARIDWKETP 60
Query: 52 ESHVFKADLPGXXXXXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRL 111
E HVFKAD+PG GN+L ISG+RS+E+E+K+DKWHRVERSSG+F+RRFRL
Sbjct: 61 EVHVFKADVPGLKKEEVKVEVDDGNILQISGERSREQEEKSDKWHRVERSSGKFLRRFRL 120
Query: 112 PENAKVDQVKAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
PEN K +Q+KA MENGVL I+ISG
Sbjct: 121 PENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQISG 159
>Os01g0135800 Similar to Cytosolic class I small heat shock protein 3B (Fragment)
Length = 150
Score = 132 bits (333), Expect = 8e-32, Method: Compositional matrix adjust.
Identities = 76/156 (48%), Positives = 90/156 (57%), Gaps = 12/156 (7%)
Query: 1 MSLVRRSNVFDPFSLDLW---DPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFK 57
MSLVR NV DP S+D W DPF +V RS+ N R+DWKETP +HVF
Sbjct: 1 MSLVRSGNVLDPMSVDFWADADPFGAV-RSLA-----ERCPVLTNVRVDWKETPTAHVFT 54
Query: 58 ADLPGXXXXXXXXXXXXGNVLVISGQRSKEKE--DKND-KWHRVERSSGQFMRRFRLPEN 114
ADLPG G VLVISG+R++E++ KND +WH VERSSG+F RRFRLP
Sbjct: 55 ADLPGVRKDQAKVEVEDGGVLVISGERAREEDVDGKNDERWHHVERSSGKFQRRFRLPRG 114
Query: 115 AKVDQVKAGMENGVLXXXXXXXXXXXXXXXXIEISG 150
A+VDQV A M+NGVL I ISG
Sbjct: 115 ARVDQVSASMDNGVLTVTVPKEETKKPQLKAIPISG 150
>Os02g0128000 Similar to Cytosolic class I small heat shock protein 6 (Fragment)
Length = 177
Score = 95.5 bits (236), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 53/124 (42%), Positives = 73/124 (58%), Gaps = 4/124 (3%)
Query: 10 FDPFSLDLWDPF-DSVFRSVVPATSDNDTAAF---ANARIDWKETPESHVFKADLPGXXX 65
+P S+D+ +PF D++ + A AA + A +DWKET +HVF AD+PG
Sbjct: 33 IEPVSVDIMEPFMDAISLTAFAAAPSAAAAAAGVPSTASMDWKETAAAHVFMADMPGVRR 92
Query: 66 XXXXXXXXXGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKAGME 125
VL ISGQR++ E+K ++WHRVERSS +F+R RLP NA D V A ++
Sbjct: 93 EEVRVEVEEEKVLRISGQRARAAEEKGERWHRVERSSERFVRTVRLPPNANTDGVHAALD 152
Query: 126 NGVL 129
NGVL
Sbjct: 153 NGVL 156
>Os04g0445100 Similar to 22.7 kDa class IV heat shock protein precursor
Length = 215
Score = 80.5 bits (197), Expect = 4e-16, Method: Compositional matrix adjust.
Identities = 44/115 (38%), Positives = 63/115 (54%), Gaps = 5/115 (4%)
Query: 19 DPFDSVFRSVVPATSDNDTAAFANARIDWKETPESHVFKADLPGXXXXXXXXXXXXGNVL 78
DPF + V +D A + AR+DW+ET ++H D+PG VL
Sbjct: 53 DPF-RILEHVPFGFDRDDVAMLSMARVDWRETGDAHEVVVDVPGMRKEDLRVEVEDNRVL 111
Query: 79 VISGQR----SKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKAGMENGVL 129
ISG+R + E++ D WHR ERS G+F R+ RLP+NA +D + A ++NGVL
Sbjct: 112 RISGERRREETTEQKGGGDHWHREERSYGRFWRQLRLPDNADLDSIAASLDNGVL 166
>Os06g0253100 HSP20-like chaperone domain containing protein
Length = 146
Score = 68.9 bits (167), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 37/99 (37%), Positives = 53/99 (53%), Gaps = 6/99 (6%)
Query: 37 TAAFANARIDWKETPESHVFKADLPGXXXXXXXXXXXXGNVLVISG---QRSKEKEDKND 93
+A A A +DW ETP SHV + ++PG GNVL + G + EKE + +
Sbjct: 24 ASASATAAMDWVETPTSHVLRINVPGLGKDDVKVQVEDGNVLTVRGAAPHAAAEKERERE 83
Query: 94 K---WHRVERSSGQFMRRFRLPENAKVDQVKAGMENGVL 129
K WH ER +F R LP +V+Q++A ++NGVL
Sbjct: 84 KDVVWHVAERGRPEFAREVALPAEVRVEQIRASVDNGVL 122
>Os11g0244200 Similar to Pisum sativum 17.9 kDa heat shock protein (hsp17.9)
(Fragment)
Length = 206
Score = 63.9 bits (154), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 38/95 (40%), Positives = 55/95 (57%), Gaps = 8/95 (8%)
Query: 43 ARIDWKETPESHVFKADLPGXXXXXXXXXXXXGN-VLVISGQRSKEKEDKND-------K 94
AR DWKETPE+HV D+PG + VL +SG+R + + + +
Sbjct: 72 ARCDWKETPEAHVVTVDVPGVRRGDVRVEVDEASRVLRVSGERRRAGAAEEEEGERDGVR 131
Query: 95 WHRVERSSGQFMRRFRLPENAKVDQVKAGMENGVL 129
WHR ER++G+F RRFR+P A V +V A +++GVL
Sbjct: 132 WHRAERAAGRFWRRFRMPPGADVGRVAARLDDGVL 166
>Os02g0217900 Similar to Cytosolic class II small heat shock protein 4 (Fragment)
Length = 175
Score = 62.8 bits (151), Expect = 8e-11, Method: Compositional matrix adjust.
Identities = 42/101 (41%), Positives = 55/101 (54%), Gaps = 7/101 (6%)
Query: 36 DTAAFANARIDWKETPESH--VFKADLPGXXXXXXXXXXXXGNVLVISGQRSKEKEDKND 93
D A AN +D KE S V D+PG GNVL ISG+R + D +D
Sbjct: 42 DRRAMANTPMDVKELRASGALVLAVDMPGVAPADVRVEVEDGNVLAISGERRRPAGDGDD 101
Query: 94 -----KWHRVERSSGQFMRRFRLPENAKVDQVKAGMENGVL 129
K+ R+ER G+FMRRF LPE+A +D V+A ++GVL
Sbjct: 102 GGEGVKYLRMERRMGKFMRRFPLPESADLDGVRAEYKDGVL 142
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.317 0.133 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,000,565
Number of extensions: 135013
Number of successful extensions: 309
Number of sequences better than 1.0e-10: 14
Number of HSP's gapped: 300
Number of HSP's successfully gapped: 14
Length of query: 150
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 58
Effective length of database: 12,232,113
Effective search space: 709462554
Effective search space used: 709462554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 151 (62.8 bits)