BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os06g0506600 Os06g0506600|AK066232
(51 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os06g0506600 Similar to Ubiquitin-conjugating enzyme E2-17 ... 111 1e-25
Os04g0667800 Ubiquitin-conjugating enzyme (EC 6.3.2.19) (Ub... 100 3e-22
Os02g0261100 Ubiquitin-conjugating enzyme OsUBC5b 100 4e-22
Os01g0819400 Similar to Ubiquitin-conjugating enzyme (EC 6.... 97 3e-21
Os01g0819500 Similar to Ubiquitin-conjugating enzyme E2-17 ... 96 5e-21
Os02g0120600 Similar to Ubiquitin-conjugating enzyme E2-17 ... 96 7e-21
Os01g0658400 Ubiquitin-conjugating enzyme OsUBC5a 92 9e-20
Os09g0293400 Similar to Ubiquitin-conjugating enzyme (EC 6.... 87 4e-18
D17786 74 2e-14
>Os06g0506600 Similar to Ubiquitin-conjugating enzyme E2-17 kDa 8 (EC 6.3.2.19)
(Ubiquitin- protein ligase 8) (Ubiquitin carrier
protein 8) (UBCAT4A)
Length = 51
Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust.
Identities = 51/51 (100%), Positives = 51/51 (100%)
Query: 1 HNFQVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
HNFQVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG
Sbjct: 1 HNFQVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
>Os04g0667800 Ubiquitin-conjugating enzyme (EC 6.3.2.19) (Ubiquitin carrier
protein)
Length = 148
Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust.
Identities = 46/48 (95%), Positives = 47/48 (97%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
+VLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTAR WTQKYAMG
Sbjct: 101 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYAMG 148
>Os02g0261100 Ubiquitin-conjugating enzyme OsUBC5b
Length = 148
Score = 99.8 bits (247), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 46/48 (95%), Positives = 47/48 (97%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
+VLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTAR WTQKYAMG
Sbjct: 101 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYAMG 148
>Os01g0819400 Similar to Ubiquitin-conjugating enzyme (EC 6.3.2.19) (Ubiquitin
carrier protein)
Length = 148
Score = 96.7 bits (239), Expect = 3e-21, Method: Compositional matrix adjust.
Identities = 44/48 (91%), Positives = 46/48 (95%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
+VLLSICSLLTDPNPDDPLVPEIAHMYKTDR KYE+TAR WTQKYAMG
Sbjct: 101 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRPKYETTARSWTQKYAMG 148
>Os01g0819500 Similar to Ubiquitin-conjugating enzyme E2-17 kDa 11 (EC
6.3.2.19) (Ubiquitin- protein ligase 11) (Ubiquitin
carrier protein 11)
Length = 47
Score = 95.9 bits (237), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 44/47 (93%), Positives = 45/47 (95%)
Query: 5 VLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
VLLSICSLLTDPNPDDPLVPEIAHMYKTDR KYE+TAR WTQKYAMG
Sbjct: 1 VLLSICSLLTDPNPDDPLVPEIAHMYKTDRPKYETTARSWTQKYAMG 47
>Os02g0120600 Similar to Ubiquitin-conjugating enzyme E2-17 kDa 9 (EC 6.3.2.19)
(Ubiquitin- protein ligase 9) (Ubiquitin carrier protein
9) (UBCAT4B)
Length = 148
Score = 95.5 bits (236), Expect = 7e-21, Method: Compositional matrix adjust.
Identities = 44/48 (91%), Positives = 46/48 (95%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
+VLLSICSLLTDPNPDDPLVPEIAHM KTDR +YESTARGWTQKYAMG
Sbjct: 101 KVLLSICSLLTDPNPDDPLVPEIAHMCKTDRLRYESTARGWTQKYAMG 148
>Os01g0658400 Ubiquitin-conjugating enzyme OsUBC5a
Length = 147
Score = 92.0 bits (227), Expect = 9e-20, Method: Compositional matrix adjust.
Identities = 42/47 (89%), Positives = 45/47 (95%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAM 50
+VLLSICSLLTDPNPDDPLVPEIAHMYKTDR KYE+TAR WTQ+YAM
Sbjct: 101 KVLLSICSLLTDPNPDDPLVPEIAHMYKTDRHKYENTARTWTQRYAM 147
>Os09g0293400 Similar to Ubiquitin-conjugating enzyme (EC 6.3.2.19) (Ubiquitin
carrier protein)
Length = 148
Score = 86.7 bits (213), Expect = 4e-18, Method: Compositional matrix adjust.
Identities = 39/48 (81%), Positives = 43/48 (89%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARGWTQKYAMG 51
+VLLSI SLLTDPNPDDPLVPEIAH+YK+ R +YE TAR WTQKYAMG
Sbjct: 101 KVLLSISSLLTDPNPDDPLVPEIAHVYKSQRPRYEETARAWTQKYAMG 148
>D17786
Length = 103
Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 35/38 (92%), Positives = 36/38 (94%)
Query: 4 QVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTA 41
+VLLSICSLLTDPN DDPLVPEIAHMYKTDRAKYE TA
Sbjct: 66 KVLLSICSLLTDPNGDDPLVPEIAHMYKTDRAKYEFTA 103
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.133 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,917,580
Number of extensions: 52886
Number of successful extensions: 178
Number of sequences better than 1.0e-10: 9
Number of HSP's gapped: 178
Number of HSP's successfully gapped: 9
Length of query: 51
Length of database: 17,035,801
Length adjustment: 24
Effective length of query: 27
Effective length of database: 15,782,665
Effective search space: 426131955
Effective search space used: 426131955
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)