BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0574300 Os05g0574300|J065204I11
(152 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0149600 Similar to Histone H2B.1 163 6e-41
Os01g0152300 Similar to Histone H2B.1 162 6e-41
Os01g0152900 Similar to Histone H2B.1 162 1e-40
Os08g0490900 Similar to Histone H2B.2 162 1e-40
Os01g0839500 Histone H2B family protein 161 2e-40
Os01g0153300 Similar to Histone H2B.1 161 2e-40
Os05g0574300 Similar to Histone H2B.2 160 3e-40
Os01g0153100 Similar to Histone H2B-3 (Fragment) 158 2e-39
Os05g0460500 Similar to Histone H2B.2 131 2e-31
AK108119 130 3e-31
AK106505 96 1e-20
Os09g0570850 Histone H2B family protein 95 2e-20
Os01g0152700 Similar to Histone H2B (CaH2B) 81 4e-16
AK108978 77 5e-15
Os08g0490800 75 2e-14
>Os01g0149600 Similar to Histone H2B.1
Length = 153
Score = 163 bits (412), Expect = 6e-41, Method: Compositional matrix adjust.
Identities = 81/92 (88%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 62 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 121
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA
Sbjct: 122 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 153
>Os01g0152300 Similar to Histone H2B.1
Length = 153
Score = 162 bits (411), Expect = 6e-41, Method: Compositional matrix adjust.
Identities = 81/92 (88%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 62 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 121
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA
Sbjct: 122 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 153
>Os01g0152900 Similar to Histone H2B.1
Length = 153
Score = 162 bits (409), Expect = 1e-40, Method: Compositional matrix adjust.
Identities = 81/92 (88%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 62 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 121
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA
Sbjct: 122 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 153
>Os08g0490900 Similar to Histone H2B.2
Length = 150
Score = 162 bits (409), Expect = 1e-40, Method: Compositional matrix adjust.
Identities = 80/92 (86%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 59 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 118
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTS+
Sbjct: 119 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS 150
>Os01g0839500 Histone H2B family protein
Length = 139
Score = 161 bits (407), Expect = 2e-40, Method: Compositional matrix adjust.
Identities = 80/92 (86%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 48 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAQEAARLARYNKKPTITSR 107
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTS+
Sbjct: 108 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS 139
>Os01g0153300 Similar to Histone H2B.1
Length = 155
Score = 161 bits (407), Expect = 2e-40, Method: Compositional matrix adjust.
Identities = 81/92 (88%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 64 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAEAAKLARYNKKPTITSR 123
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA
Sbjct: 124 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 155
>Os05g0574300 Similar to Histone H2B.2
Length = 152
Score = 160 bits (405), Expect = 3e-40, Method: Compositional matrix adjust.
Identities = 81/92 (88%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAEAAKLARYNKKPTITSR 120
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA
Sbjct: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
>Os01g0153100 Similar to Histone H2B-3 (Fragment)
Length = 153
Score = 158 bits (399), Expect = 2e-39, Method: Compositional matrix adjust.
Identities = 79/92 (85%), Positives = 81/92 (88%)
Query: 61 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSR 120
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSR
Sbjct: 62 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAEAAKLARYNKKPTITSR 121
Query: 121 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
EIQT+VRLVLPGELAKHAVSEGTKAVTKFTS+
Sbjct: 122 EIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 153
>Os05g0460500 Similar to Histone H2B.2
Length = 118
Score = 131 bits (330), Expect = 2e-31, Method: Compositional matrix adjust.
Identities = 64/77 (83%), Positives = 66/77 (85%)
Query: 76 VHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREIQTSVRLVLPGELA 135
VHPDIGISSKAMSIMNSF+NDIF RYNKKPTITSREIQTSVRLVLPGELA
Sbjct: 42 VHPDIGISSKAMSIMNSFVNDIFEKLEQEAARLARYNKKPTITSREIQTSVRLVLPGELA 101
Query: 136 KHAVSEGTKAVTKFTSA 152
KHAVSEGTKAVTKFTS+
Sbjct: 102 KHAVSEGTKAVTKFTSS 118
>AK108119
Length = 142
Score = 130 bits (328), Expect = 3e-31, Method: Compositional matrix adjust.
Identities = 59/90 (65%), Positives = 73/90 (81%)
Query: 63 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREI 122
ETY YI++VLKQVHPD GIS+KAM+I+NSF+ DIF YNKK TI+SREI
Sbjct: 52 ETYSTYIYRVLKQVHPDTGISNKAMAILNSFVQDIFERIATEASKLASYNKKSTISSREI 111
Query: 123 QTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
QT+VRL+LPGEL+KHA+SEGTK+VTKF+S+
Sbjct: 112 QTAVRLILPGELSKHAISEGTKSVTKFSSS 141
>AK106505
Length = 194
Score = 95.5 bits (236), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 39/87 (44%), Positives = 62/87 (71%)
Query: 65 YKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREIQT 124
YK Y+++VLKQVHPD+G S++ M +++ + D+F + + + T+TSRE+Q+
Sbjct: 108 YKRYVWRVLKQVHPDLGASAQTMDVLDMMMADMFERLADEASRLSKLSGRLTLTSREVQS 167
Query: 125 SVRLVLPGELAKHAVSEGTKAVTKFTS 151
+VRLVLP +LA HA+SEGTKA++ + S
Sbjct: 168 AVRLVLPADLANHAISEGTKAISNYLS 194
>Os09g0570850 Histone H2B family protein
Length = 194
Score = 95.1 bits (235), Expect = 2e-20, Method: Compositional matrix adjust.
Identities = 39/87 (44%), Positives = 62/87 (71%)
Query: 65 YKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREIQT 124
YK Y+++VLKQVHPD+G S++ M +++ + D+F + + + T+TSRE+Q+
Sbjct: 108 YKRYVWRVLKQVHPDLGASAQTMDVLDMMMADMFERLADEASRLSKLSGRLTLTSREVQS 167
Query: 125 SVRLVLPGELAKHAVSEGTKAVTKFTS 151
+VRLVLP +LA HA+SEGTKA++ + S
Sbjct: 168 AVRLVLPADLANHAISEGTKAISNYLS 194
>Os01g0152700 Similar to Histone H2B (CaH2B)
Length = 41
Score = 80.9 bits (198), Expect = 4e-16, Method: Compositional matrix adjust.
Identities = 39/41 (95%), Positives = 41/41 (100%)
Query: 112 NKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA 152
NKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTKFTS+
Sbjct: 1 NKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 41
>AK108978
Length = 320
Score = 77.0 bits (188), Expect = 5e-15, Method: Compositional matrix adjust.
Identities = 35/83 (42%), Positives = 54/83 (65%)
Query: 69 IFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREIQTSVRL 128
+++ LK+V P++GIS+K M+++NS ++D+ RYN + T+T+R IQT+VRL
Sbjct: 8 VYRALKKVDPEMGISAKGMAVVNSLVHDVLARLAVEAGRLARYNARKTVTARHIQTAVRL 67
Query: 129 VLPGELAKHAVSEGTKAVTKFTS 151
+LP L K AV GT+AV S
Sbjct: 68 LLPLGLDKGAVVAGTEAVEAHRS 90
>Os08g0490800
Length = 79
Score = 74.7 bits (182), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 36/38 (94%), Positives = 36/38 (94%)
Query: 110 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVT 147
RYNKKPTITSREIQTSVRLVL GELAKHAVSEG KAVT
Sbjct: 14 RYNKKPTITSREIQTSVRLVLLGELAKHAVSEGAKAVT 51
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.131 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,902,761
Number of extensions: 36694
Number of successful extensions: 75
Number of sequences better than 1.0e-10: 15
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 15
Length of query: 152
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 60
Effective length of database: 12,232,113
Effective search space: 733926780
Effective search space used: 733926780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)