BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0686800 Os02g0686800|AK071055
(146 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0686800 Protein of unknown function DUF581 family protein 302 6e-83
Os04g0585900 Protein of unknown function DUF581 family protein 81 3e-16
Os04g0585700 Protein of unknown function DUF581 family protein 77 3e-15
Os04g0586000 Protein of unknown function DUF581 family protein 75 1e-14
AK060089 75 1e-14
Os02g0686700 Protein of unknown function DUF581 family protein 74 3e-14
Os02g0686900 72 2e-13
Os04g0586100 Protein of unknown function DUF581 family protein 72 2e-13
Os02g0687200 Protein of unknown function DUF581 family protein 63 1e-10
>Os02g0686800 Protein of unknown function DUF581 family protein
Length = 146
Score = 302 bits (774), Expect = 6e-83, Method: Compositional matrix adjust.
Identities = 146/146 (100%), Positives = 146/146 (100%)
Query: 1 MDFTSSYFHAFGNPDFAAVFSGGGSAQAIRPGTTTSSSGGAKAVNVGRGGAARQGAPSVF 60
MDFTSSYFHAFGNPDFAAVFSGGGSAQAIRPGTTTSSSGGAKAVNVGRGGAARQGAPSVF
Sbjct: 1 MDFTSSYFHAFGNPDFAAVFSGGGSAQAIRPGTTTSSSGGAKAVNVGRGGAARQGAPSVF 60
Query: 61 CVQDAEVEEAHHFLDECTLCRKGLAGDIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQ 120
CVQDAEVEEAHHFLDECTLCRKGLAGDIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQ
Sbjct: 61 CVQDAEVEEAHHFLDECTLCRKGLAGDIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQ 120
Query: 121 YSPTAQAAAHHHRSERAPQRQLQPQR 146
YSPTAQAAAHHHRSERAPQRQLQPQR
Sbjct: 121 YSPTAQAAAHHHRSERAPQRQLQPQR 146
>Os04g0585900 Protein of unknown function DUF581 family protein
Length = 91
Score = 81.3 bits (199), Expect = 3e-16, Method: Compositional matrix adjust.
Identities = 38/55 (69%), Positives = 45/55 (81%), Gaps = 7/55 (12%)
Query: 76 ECTLCRKGLAGDIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYSPTAQAAAH 130
EC+LC K L+GDIFMYRGDTPFCSEECR++QIE+DR +HRRKK +AAAH
Sbjct: 1 ECSLCGKSLSGDIFMYRGDTPFCSEECRQQQIEVDRAKHRRKK-------RAAAH 48
>Os04g0585700 Protein of unknown function DUF581 family protein
Length = 150
Score = 77.4 bits (189), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 35/53 (66%), Positives = 40/53 (75%), Gaps = 2/53 (3%)
Query: 72 HFLDECTLCRKGLAG--DIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYS 122
HFL+ C LCRK LA DI+MYRGD PFCSEECRREQIEMD R++K+ S
Sbjct: 68 HFLEACFLCRKPLASNRDIYMYRGDIPFCSEECRREQIEMDDEMERKEKKNIS 120
>Os04g0586000 Protein of unknown function DUF581 family protein
Length = 127
Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 50/128 (39%), Positives = 65/128 (50%), Gaps = 27/128 (21%)
Query: 15 DFAAVFSGGGSAQAIRPGTTTSSSGGAKAVNVGRGGAARQGAPSVFCVQDAEVEEA---H 71
D A FSG SA ++P + GG +FC + ++ H
Sbjct: 12 DIEAGFSGH-SASPVKPAASPRRPGG-----------------RLFCDPCDDADDLLGHH 53
Query: 72 HFLDECTLCRKGLAG--DIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYSPTAQAAA 129
H+LD C CR+ L G DIFMYRGD PFCSEECR+EQIE+D R +R KQ T +A
Sbjct: 54 HYLDICFRCRRPLGGNRDIFMYRGDMPFCSEECRQEQIEIDEAREQRSKQ----TGRAEQ 109
Query: 130 HHHRSERA 137
R ++A
Sbjct: 110 ERQRQQKA 117
>AK060089
Length = 127
Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 50/128 (39%), Positives = 65/128 (50%), Gaps = 27/128 (21%)
Query: 15 DFAAVFSGGGSAQAIRPGTTTSSSGGAKAVNVGRGGAARQGAPSVFCVQDAEVEEA---H 71
D A FSG SA ++P + GG +FC + ++ H
Sbjct: 12 DIEAGFSGH-SASPVKPAASPRRPGG-----------------RLFCDPCDDADDLLGHH 53
Query: 72 HFLDECTLCRKGLAG--DIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYSPTAQAAA 129
H+LD C CR+ L G DIFMYRGD PFCSEECR+EQIE+D R +R KQ T +A
Sbjct: 54 HYLDICFRCRRPLGGNRDIFMYRGDMPFCSEECRQEQIEIDEAREQRSKQ----TGRAEQ 109
Query: 130 HHHRSERA 137
R ++A
Sbjct: 110 ERQRQQKA 117
>Os02g0686700 Protein of unknown function DUF581 family protein
Length = 92
Score = 74.3 bits (181), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 39/66 (59%), Positives = 43/66 (65%), Gaps = 2/66 (3%)
Query: 72 HFLDECTLCRKGLAG--DIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYSPTAQAAA 129
HFLD C LCRK LAG DIFMYRGDTPFCSEECRREQ+E D R +K + + A
Sbjct: 8 HFLDACFLCRKRLAGNRDIFMYRGDTPFCSEECRREQMEADAAAERTEKARRAGKLTRGA 67
Query: 130 HHHRSE 135
R E
Sbjct: 68 PSSRRE 73
>Os02g0686900
Length = 125
Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 40/76 (52%), Positives = 47/76 (61%), Gaps = 7/76 (9%)
Query: 71 HHFLDECTLCRKGLAG--DIFMYRGDTPFCSEECRREQIEMDRNRHRRKKQQYSPTAQAA 128
HH+LD C LC + LAG DIFMYRGDTPFCSEECR+ QI+ D KK+ A A
Sbjct: 47 HHYLDACFLCGRMLAGNKDIFMYRGDTPFCSEECRQRQIDADDASEMMKKRAKMQPAAA- 105
Query: 129 AHHHRSERAPQRQLQP 144
R E+ PQR+ P
Sbjct: 106 ----RGEQQPQRRQSP 117
>Os04g0586100 Protein of unknown function DUF581 family protein
Length = 97
Score = 71.6 bits (174), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 37/76 (48%), Positives = 48/76 (63%), Gaps = 6/76 (7%)
Query: 68 EEAHHFLDECTLCRKGLA--GDIFMYRGDTPFCSEECRREQIEMD----RNRHRRKKQQY 121
E+ H LD C LC K L DIFMY+GDTPFCSEECR EQ+++D R+ RR++QQY
Sbjct: 18 EQGRHALDACALCTKQLTRDSDIFMYKGDTPFCSEECRYEQMQLDAVYARSAGRRQQQQY 77
Query: 122 SPTAQAAAHHHRSERA 137
S A + H + +
Sbjct: 78 SGRADSRRGHRETRKV 93
>Os02g0687200 Protein of unknown function DUF581 family protein
Length = 106
Score = 62.8 bits (151), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 28/41 (68%), Positives = 32/41 (78%), Gaps = 2/41 (4%)
Query: 72 HFLDECTLCRKGLA--GDIFMYRGDTPFCSEECRREQIEMD 110
H +D C+LCRK L DIFMYRG+TPFCSEECR Q+EMD
Sbjct: 27 HAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMD 67
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.132 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,146,450
Number of extensions: 284721
Number of successful extensions: 1845
Number of sequences better than 1.0e-10: 11
Number of HSP's gapped: 1843
Number of HSP's successfully gapped: 11
Length of query: 146
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 55
Effective length of database: 12,284,327
Effective search space: 675637985
Effective search space used: 675637985
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)