BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0571000 Os12g0571000|J100025H13
(65 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0571000 Plant metallothionein, family 15 protein 132 7e-32
Os12g0570700 Similar to Metallothionein-like protein type 1 79 7e-16
Os12g0571100 Similar to Metallothionein-like protein type 1 77 2e-15
Os12g0567800 Plant metallothionein, family 15 protein 77 2e-15
Os03g0288000 Similar to Metallothionein 77 3e-15
Os11g0704500 Metallothionein-like protein type 1 75 8e-15
>Os12g0571000 Plant metallothionein, family 15 protein
Length = 65
Score = 132 bits (331), Expect = 7e-32, Method: Compositional matrix adjust.
Identities = 65/65 (100%), Positives = 65/65 (100%)
Query: 1 AAAAGKMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKC 60
AAAAGKMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKC
Sbjct: 1 AAAAGKMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKC 60
Query: 61 NPCNC 65
NPCNC
Sbjct: 61 NPCNC 65
>Os12g0570700 Similar to Metallothionein-like protein type 1
Length = 78
Score = 79.0 bits (193), Expect = 7e-16, Method: Compositional matrix adjust.
Identities = 44/61 (72%), Positives = 47/61 (77%), Gaps = 3/61 (4%)
Query: 5 GKMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCN 64
G MYPDLAEK T TS TM+LGVAP K + E V +AA SGEA HGCSCGSSCKCNPCN
Sbjct: 21 GNMYPDLAEK--TTNTSATMVLGVAPAKEQFEGVGKAAE-SGEAAHGCSCGSSCKCNPCN 77
Query: 65 C 65
C
Sbjct: 78 C 78
>Os12g0571100 Similar to Metallothionein-like protein type 1
Length = 78
Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 45/60 (75%), Positives = 49/60 (81%), Gaps = 1/60 (1%)
Query: 6 KMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCNC 65
KMYPDLAEK TT TT+ T +LGVAPEKG E V +AA SGEA HGCSCGSSC+CNPCNC
Sbjct: 20 KMYPDLAEKITTTTTTATTVLGVAPEKGHSEGVGKAAE-SGEAAHGCSCGSSCRCNPCNC 78
>Os12g0567800 Plant metallothionein, family 15 protein
Length = 78
Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 44/60 (73%), Positives = 48/60 (80%), Gaps = 1/60 (1%)
Query: 6 KMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCNC 65
KMYPDLAEK T TT+ T +LGVAPEKG+ E V +AA SGE HGCSCGSSCKCNPCNC
Sbjct: 20 KMYPDLAEKITITTTTTTTVLGVAPEKGQFEGVGKAAE-SGEGAHGCSCGSSCKCNPCNC 78
>Os03g0288000 Similar to Metallothionein
Length = 72
Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 41/61 (67%), Positives = 46/61 (75%), Gaps = 6/61 (9%)
Query: 5 GKMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCN 64
GKMYPDL EK+++ T++LGVAPEK A FEAAA SGE HGC CGSSCKCNPCN
Sbjct: 18 GKMYPDLEEKSSSAQA--TVVLGVAPEK----AHFEAAAESGETAHGCGCGSSCKCNPCN 71
Query: 65 C 65
C
Sbjct: 72 C 72
>Os11g0704500 Metallothionein-like protein type 1
Length = 74
Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust.
Identities = 39/60 (65%), Positives = 46/60 (76%), Gaps = 4/60 (6%)
Query: 6 KMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCNC 65
K YPDL EK+++ T T++LGVAPEK + FEAAA SGE HGCSCGSSC+CNPCNC
Sbjct: 19 KKYPDLEEKSSS--TKATVVLGVAPEKKAQQ--FEAAAESGETAHGCSCGSSCRCNPCNC 74
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.313 0.127 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,574,853
Number of extensions: 89593
Number of successful extensions: 302
Number of sequences better than 1.0e-10: 9
Number of HSP's gapped: 292
Number of HSP's successfully gapped: 9
Length of query: 65
Length of database: 17,035,801
Length adjustment: 37
Effective length of query: 28
Effective length of database: 15,103,883
Effective search space: 422908724
Effective search space used: 422908724
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)