BLAST Search Results

BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0207300 Os12g0207300|AK069232
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0207300  Similar to Clathrin coat assembly protein AP17...   288   1e-78
Os03g0811300  Similar to Clathrin assembly small subunit pro...   167   3e-42
Os03g0783700  Similar to Clathrin assembly small subunit pro...   165   1e-41
Os10g0368400  Similar to Adapter-related protein complex 4 s...   125   1e-29
Os03g0228400  Similar to Adapter-related protein complex 3 s...   107   4e-24
Os08g0395300  Clathrin adaptor complex, small chain family p...    96   6e-21

>Os12g0207300 Similar to Clathrin coat assembly protein AP17 (Clathrin coat
           associated protein AP17) (Plasma membrane adaptor AP-2
           17 kDa protein) (HA2 17 kDa subunit) (Clathrin assembly
           protein 2 small chain)
          Length = 142

 Score =  288 bits (737), Expect = 1e-78,   Method: Compositional matrix adjust.
 Identities = 142/142 (100%), Positives = 142/142 (100%)

Query: 1   MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYR 60
           MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYR
Sbjct: 1   MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYR 60

Query: 61  RYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILA 120
           RYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILA
Sbjct: 61  RYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILA 120

Query: 121 GELQETSKRAIIERMGELEKLE 142
           GELQETSKRAIIERMGELEKLE
Sbjct: 121 GELQETSKRAIIERMGELEKLE 142



>Os03g0811300 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
           assembly protein AP19, small subunit)
          Length = 169

 Score =  167 bits (422), Expect = 3e-42,   Method: Compositional matrix adjust.
 Identities = 76/132 (57%), Positives = 99/132 (75%)

Query: 1   MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYR 60
           MI F+LL +RQGK RL K+Y P    E+ KV  EV  LV+ R PK  NFV++  ++V+Y+
Sbjct: 9   MIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWHGYRVVYK 68

Query: 61  RYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILA 120
           RYA L+F MC+D  DNEL  L+ IH FVEILD +F NVCELDL+FNFHK Y ILDE ++A
Sbjct: 69  RYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYFILDEVLIA 128

Query: 121 GELQETSKRAII 132
           GELQE++K+A++
Sbjct: 129 GELQESNKKAVL 140



>Os03g0783700 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
           assembly protein AP19, small subunit)
          Length = 182

 Score =  165 bits (417), Expect = 1e-41,   Method: Compositional matrix adjust.
 Identities = 76/140 (54%), Positives = 100/140 (71%)

Query: 2   IRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRR 61
           I F+LL +RQGK RL K+Y P    E+ KV  E+  L++ R PK  NFVE++ +KV+YRR
Sbjct: 23  INFVLLISRQGKVRLTKWYSPYTQKERTKVIRELSGLILTRGPKLCNFVEWKGYKVVYRR 82

Query: 62  YAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAG 121
           YA L+F MC+D  DNEL  LE IH FVE+LD +F +VCELDL+FNFHK Y ILDE ++AG
Sbjct: 83  YASLYFCMCIDAEDNELEVLEIIHHFVEVLDRYFGSVCELDLIFNFHKAYYILDEILIAG 142

Query: 122 ELQETSKRAIIERMGELEKL 141
           ELQE+SK+ +   +   + L
Sbjct: 143 ELQESSKKNVARLIAAQDSL 162



>Os10g0368400 Similar to Adapter-related protein complex 4 sigma 1 subunit (Sigma
           subunit of AP-4) (AP-4 adapter complex sigma subunit)
          Length = 143

 Score =  125 bits (314), Expect = 1e-29,   Method: Compositional matrix adjust.
 Identities = 65/139 (46%), Positives = 95/139 (68%)

Query: 2   IRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRR 61
           IRF+++ N+QG+TR+A+YY  L   E+  +E E+ R  + R     +FVE R +KV+YRR
Sbjct: 3   IRFVVMVNKQGQTRVAQYYEHLSVDERRALEGEIVRKCLARTDHQCSFVEHRNYKVVYRR 62

Query: 62  YAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAG 121
           YA LFF + VD  +NELA LE IHL VE +D  F NVCELD++F+  KV+ +L+E ++ G
Sbjct: 63  YASLFFLVGVDNDENELAILEFIHLLVETMDRHFGNVCELDIMFHLEKVHFMLEEMVMNG 122

Query: 122 ELQETSKRAIIERMGELEK 140
            + ETSK+ I+  +  +EK
Sbjct: 123 CIVETSKQNILAPIHLMEK 141



>Os03g0228400 Similar to Adapter-related protein complex 3 sigma 2 subunit
           (Sigma-adaptin 3b) (AP-3 complex sigma-3B subunit)
           (Sigma-3B-adaptin)
          Length = 166

 Score =  107 bits (266), Expect = 4e-24,   Method: Compositional matrix adjust.
 Identities = 60/148 (40%), Positives = 92/148 (62%), Gaps = 7/148 (4%)

Query: 1   MIRFILLQNRQGKTRLAKYYVPLEDSEKHK-VEYEVHRLVVNRDPKFTNFVEFRT----- 54
           MI+ +++ + QGK RL K+Y   +  EKH+ +   V +L+  R    +NFV+        
Sbjct: 1   MIQAVMVMSTQGKPRLLKFY-SYQPPEKHQDLVRGVFQLLSARPDSVSNFVKVDAIFGPG 59

Query: 55  HKVIYRRYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLIL 114
            K++Y+  A L+F    D ++NELA L+ + +FVE LD  F NVCELD+VFNF+K++ IL
Sbjct: 60  AKLVYKHLATLYFVFVFDSSENELAVLDLVQVFVETLDRCFKNVCELDIVFNFNKLHTIL 119

Query: 115 DEFILAGELQETSKRAIIERMGELEKLE 142
           DE IL G++ ETS   I+  + E+ +LE
Sbjct: 120 DEMILGGQVIETSSEQIMRSVEEIARLE 147



>Os08g0395300 Clathrin adaptor complex, small chain family protein
          Length = 100

 Score = 96.3 bits (238), Expect = 6e-21,   Method: Compositional matrix adjust.
 Identities = 51/97 (52%), Positives = 66/97 (68%)

Query: 2  IRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRR 61
          IRF+L  N+QG+TRLA+YY  L   E+  +E E+ R  + R  +  +FVE R +KV+YRR
Sbjct: 3  IRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVYRR 62

Query: 62 YAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNV 98
          YA LFF + VD  +NELA LE IHLFVE +D  F NV
Sbjct: 63 YASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.329    0.145    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,281,172
Number of extensions: 161581
Number of successful extensions: 437
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 436
Number of HSP's successfully gapped: 6
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 151 (62.8 bits)