BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0395300 Os08g0395300|J065158M06
(100 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0395300 Clathrin adaptor complex, small chain family p... 204 9e-54
Os10g0368400 Similar to Adapter-related protein complex 4 s... 196 4e-51
Os03g0783700 Similar to Clathrin assembly small subunit pro... 99 8e-22
Os12g0207300 Similar to Clathrin coat assembly protein AP17... 96 4e-21
Os03g0811300 Similar to Clathrin assembly small subunit pro... 94 3e-20
Os03g0228400 Similar to Adapter-related protein complex 3 s... 65 8e-12
>Os08g0395300 Clathrin adaptor complex, small chain family protein
Length = 100
Score = 204 bits (520), Expect = 9e-54, Method: Compositional matrix adjust.
Identities = 100/100 (100%), Positives = 100/100 (100%)
Query: 1 MGIRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVY 60
MGIRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVY
Sbjct: 1 MGIRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVY 60
Query: 61 RRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNVV 100
RRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNVV
Sbjct: 61 RRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNVV 100
>Os10g0368400 Similar to Adapter-related protein complex 4 sigma 1 subunit
(Sigma subunit of AP-4) (AP-4 adapter complex sigma
subunit)
Length = 143
Score = 196 bits (497), Expect = 4e-51, Method: Compositional matrix adjust.
Identities = 93/99 (93%), Positives = 96/99 (96%)
Query: 1 MGIRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVY 60
MGIRFV+ VNKQGQTR+AQYYEHLS+DERRALEGEIVRKCLARTD QCSFVEHRNYKVVY
Sbjct: 1 MGIRFVVMVNKQGQTRVAQYYEHLSVDERRALEGEIVRKCLARTDHQCSFVEHRNYKVVY 60
Query: 61 RRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99
RRYASLFFLVGVDNDENELAILEFIHL VETMDRHFGNV
Sbjct: 61 RRYASLFFLVGVDNDENELAILEFIHLLVETMDRHFGNV 99
>Os03g0783700 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
assembly protein AP19, small subunit)
Length = 182
Score = 98.6 bits (244), Expect = 8e-22, Method: Compositional matrix adjust.
Identities = 46/99 (46%), Positives = 68/99 (68%)
Query: 1 MGIRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVY 60
+ I FVL +++QG+ RL ++Y + ER + E+ L R + C+FVE + YKVVY
Sbjct: 21 LQINFVLLISRQGKVRLTKWYSPYTQKERTKVIRELSGLILTRGPKLCNFVEWKGYKVVY 80
Query: 61 RRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99
RRYASL+F + +D ++NEL +LE IH FVE +DR+FG+V
Sbjct: 81 RRYASLYFCMCIDAEDNELEVLEIIHHFVEVLDRYFGSV 119
>Os12g0207300 Similar to Clathrin coat assembly protein AP17 (Clathrin coat
associated protein AP17) (Plasma membrane adaptor AP-2
17 kDa protein) (HA2 17 kDa subunit) (Clathrin assembly
protein 2 small chain)
Length = 142
Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust.
Identities = 51/97 (52%), Positives = 66/97 (68%)
Query: 3 IRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVYRR 62
IRF+L N+QG+TRLA+YY L E+ +E E+ R + R + +FVE R +KV+YRR
Sbjct: 2 IRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRR 61
Query: 63 YASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99
YA LFF + VD +NELA LE IHLFVE +D F NV
Sbjct: 62 YAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNV 98
>Os03g0811300 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
assembly protein AP19, small subunit)
Length = 169
Score = 93.6 bits (231), Expect = 3e-20, Method: Compositional matrix adjust.
Identities = 43/97 (44%), Positives = 63/97 (64%)
Query: 3 IRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVYRR 62
I FVL +++QG+ RL ++Y ER + E+ L R + C+FV+ Y+VVY+R
Sbjct: 10 IHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWHGYRVVYKR 69
Query: 63 YASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99
YASL+F + +D +NEL L+ IH FVE +DR+FGNV
Sbjct: 70 YASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNV 106
>Os03g0228400 Similar to Adapter-related protein complex 3 sigma 2 subunit
(Sigma-adaptin 3b) (AP-3 complex sigma-3B subunit)
(Sigma-3B-adaptin)
Length = 166
Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust.
Identities = 35/102 (34%), Positives = 61/102 (59%), Gaps = 5/102 (4%)
Query: 3 IRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVE-----HRNYK 57
I+ V+ ++ QG+ RL ++Y + ++ + L + + AR D +FV+ K
Sbjct: 2 IQAVMVMSTQGKPRLLKFYSYQPPEKHQDLVRGVFQLLSARPDSVSNFVKVDAIFGPGAK 61
Query: 58 VVYRRYASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99
+VY+ A+L+F+ D+ ENELA+L+ + +FVET+DR F NV
Sbjct: 62 LVYKHLATLYFVFVFDSSENELAVLDLVQVFVETLDRCFKNV 103
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.330 0.143 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,946,330
Number of extensions: 97816
Number of successful extensions: 193
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 192
Number of HSP's successfully gapped: 6
Length of query: 100
Length of database: 17,035,801
Length adjustment: 69
Effective length of query: 31
Effective length of database: 13,433,035
Effective search space: 416424085
Effective search space used: 416424085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)