BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0365700 Os05g0365700|AK120998
(85 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0365700 Glycoside hydrolase, family 1 protein 118 1e-27
Os05g0366000 Glycoside hydrolase, family 1 protein 93 4e-20
Os05g0365600 Similar to Hydroxyisourate hydrolase 92 9e-20
Os05g0366600 Similar to Hydroxyisourate hydrolase 74 3e-14
Os09g0511700 Similar to Prunasin hydrolase isoform PH C pre... 73 6e-14
Os01g0813700 Similar to Hydroxyisourate hydrolase 67 2e-12
Os09g0511600 Glycoside hydrolase, family 1 protein 67 4e-12
Os09g0511900 Glycoside hydrolase, family 1 protein 66 5e-12
>Os05g0365700 Glycoside hydrolase, family 1 protein
Length = 85
Score = 118 bits (295), Expect = 1e-27, Method: Compositional matrix adjust.
Identities = 67/85 (78%), Positives = 67/85 (78%)
Query: 1 MERRPXXXXXXXXXXXXXXXXXXQGVSSLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGR 60
MERRP QGVSSLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGR
Sbjct: 1 MERRPLHLHLLLFFSAWLLLLLLQGVSSLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGR 60
Query: 61 TPSIWDTYTHSGLSLYISQFVLELG 85
TPSIWDTYTHSGLSLYISQFVLELG
Sbjct: 61 TPSIWDTYTHSGLSLYISQFVLELG 85
>Os05g0366000 Glycoside hydrolase, family 1 protein
Length = 451
Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 43/49 (87%), Positives = 45/49 (91%)
Query: 24 QGVSSLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSG 72
QGVSSLQF R+DFP FAFGAGT+AYQYEG AAEDGRTPSIWDTYTHSG
Sbjct: 21 QGVSSLQFTRDDFPHDFAFGAGTSAYQYEGGAAEDGRTPSIWDTYTHSG 69
>Os05g0365600 Similar to Hydroxyisourate hydrolase
Length = 528
Score = 92.0 bits (227), Expect = 9e-20, Method: Compositional matrix adjust.
Identities = 42/48 (87%), Positives = 44/48 (91%)
Query: 24 QGVSSLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHS 71
QGV SLQF R+DFPDGF FGAGTAA+QYEGAAAEDGRTPSIWDTY HS
Sbjct: 37 QGVRSLQFTRDDFPDGFTFGAGTAAFQYEGAAAEDGRTPSIWDTYAHS 84
>Os05g0366600 Similar to Hydroxyisourate hydrolase
Length = 533
Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 32/44 (72%), Positives = 37/44 (84%)
Query: 29 LQFRREDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSG 72
L F R+DFP F FGAGT+AYQYEGA EDGR+PSIWDT+TH+G
Sbjct: 40 LNFTRQDFPGEFVFGAGTSAYQYEGATDEDGRSPSIWDTFTHAG 83
>Os09g0511700 Similar to Prunasin hydrolase isoform PH C precursor (EC
3.2.1.118)
Length = 274
Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust.
Identities = 32/42 (76%), Positives = 36/42 (85%)
Query: 33 REDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSGLS 74
R DFP+GF FGAGT+A+Q EGAAAEDGR PSIWDT+TH G S
Sbjct: 33 RHDFPEGFVFGAGTSAFQVEGAAAEDGRKPSIWDTFTHQGYS 74
>Os01g0813700 Similar to Hydroxyisourate hydrolase
Length = 118
Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 28/45 (62%), Positives = 36/45 (80%)
Query: 28 SLQFRREDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSG 72
++ + R DFP F FGA T+AYQY+GAAAEDGR+P+IWDT+ H G
Sbjct: 22 AVGYTRSDFPRDFVFGAATSAYQYDGAAAEDGRSPTIWDTFAHEG 66
>Os09g0511600 Glycoside hydrolase, family 1 protein
Length = 523
Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust.
Identities = 30/43 (69%), Positives = 35/43 (81%)
Query: 33 REDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSGLSL 75
R DFP F FGAG++AYQ EGA AEDGR PSIWDT++HSG S+
Sbjct: 32 RADFPPEFIFGAGSSAYQVEGAFAEDGRKPSIWDTFSHSGYSV 74
>Os09g0511900 Glycoside hydrolase, family 1 protein
Length = 507
Score = 66.2 bits (160), Expect = 5e-12, Method: Compositional matrix adjust.
Identities = 28/40 (70%), Positives = 34/40 (85%)
Query: 33 REDFPDGFAFGAGTAAYQYEGAAAEDGRTPSIWDTYTHSG 72
R DFP+GF FGAG++A+Q EGAAAEDGR PSIWDT+ + G
Sbjct: 37 RHDFPEGFVFGAGSSAFQVEGAAAEDGRKPSIWDTFINQG 76
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.136 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,433,461
Number of extensions: 85719
Number of successful extensions: 193
Number of sequences better than 1.0e-10: 9
Number of HSP's gapped: 194
Number of HSP's successfully gapped: 9
Length of query: 85
Length of database: 17,035,801
Length adjustment: 55
Effective length of query: 30
Effective length of database: 14,164,031
Effective search space: 424920930
Effective search space used: 424920930
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)