BLAST Search Results

BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0811300 Os03g0811300|AK071871
         (169 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0811300  Similar to Clathrin assembly small subunit pro...   353   4e-98
Os03g0783700  Similar to Clathrin assembly small subunit pro...   289   6e-79
Os12g0207300  Similar to Clathrin coat assembly protein AP17...   167   4e-42
Os10g0368400  Similar to Adapter-related protein complex 4 s...   119   9e-28
Os03g0228400  Similar to Adapter-related protein complex 3 s...   113   8e-26
Os08g0395300  Clathrin adaptor complex, small chain family p...    94   6e-20

>Os03g0811300 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
           assembly protein AP19, small subunit)
          Length = 169

 Score =  353 bits (905), Expect = 4e-98,   Method: Compositional matrix adjust.
 Identities = 169/169 (100%), Positives = 169/169 (100%)

Query: 1   PAPPPSLVMIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDW 60
           PAPPPSLVMIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDW
Sbjct: 1   PAPPPSLVMIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDW 60

Query: 61  HGYRVVYKRYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYF 120
           HGYRVVYKRYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYF
Sbjct: 61  HGYRVVYKRYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYF 120

Query: 121 ILDEVLIAGELQESNKKAVLRLITTQDNLVEAAKEEASSLRNIIAQATK 169
           ILDEVLIAGELQESNKKAVLRLITTQDNLVEAAKEEASSLRNIIAQATK
Sbjct: 121 ILDEVLIAGELQESNKKAVLRLITTQDNLVEAAKEEASSLRNIIAQATK 169



>Os03g0783700 Similar to Clathrin assembly small subunit protein AP19 (Clathrin
           assembly protein AP19, small subunit)
          Length = 182

 Score =  289 bits (740), Expect = 6e-79,   Method: Compositional matrix adjust.
 Identities = 134/169 (79%), Positives = 153/169 (90%)

Query: 1   PAPPPSLVMIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDW 60
           P     L+ I+FVLLISRQGKVRLTKWY+PY QKER+KVI+E+S L+LTRGPK+CNFV+W
Sbjct: 14  PFAVVGLLQINFVLLISRQGKVRLTKWYSPYTQKERTKVIRELSGLILTRGPKLCNFVEW 73

Query: 61  HGYRVVYKRYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYF 120
            GY+VVY+RYASLYFCMCIDA DNELE L+IIHHFVE+LDRYFG+VCELDLIFNFHKAY+
Sbjct: 74  KGYKVVYRRYASLYFCMCIDAEDNELEVLEIIHHFVEVLDRYFGSVCELDLIFNFHKAYY 133

Query: 121 ILDEVLIAGELQESNKKAVLRLITTQDNLVEAAKEEASSLRNIIAQATK 169
           ILDE+LIAGELQES+KK V RLI  QD+LVEAAKEEASS+ NIIAQATK
Sbjct: 134 ILDEILIAGELQESSKKNVARLIAAQDSLVEAAKEEASSISNIIAQATK 182



>Os12g0207300 Similar to Clathrin coat assembly protein AP17 (Clathrin coat
           associated protein AP17) (Plasma membrane adaptor AP-2
           17 kDa protein) (HA2 17 kDa subunit) (Clathrin assembly
           protein 2 small chain)
          Length = 142

 Score =  167 bits (422), Expect = 4e-42,   Method: Compositional matrix adjust.
 Identities = 76/132 (57%), Positives = 99/132 (75%)

Query: 9   MIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWHGYRVVYK 68
           MI F+LL +RQGK RL K+Y P    E+ KV  EV  LV+ R PK  NFV++  ++V+Y+
Sbjct: 1   MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYR 60

Query: 69  RYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYFILDEVLIA 128
           RYA L+F MC+D  DNEL  L+ IH FVEILD +F NVCELDL+FNFHK Y ILDE ++A
Sbjct: 61  RYAGLFFSMCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILA 120

Query: 129 GELQESNKKAVL 140
           GELQE++K+A++
Sbjct: 121 GELQETSKRAII 132



>Os10g0368400 Similar to Adapter-related protein complex 4 sigma 1 subunit (Sigma
           subunit of AP-4) (AP-4 adapter complex sigma subunit)
          Length = 143

 Score =  119 bits (298), Expect = 9e-28,   Method: Compositional matrix adjust.
 Identities = 53/131 (40%), Positives = 89/131 (67%)

Query: 10  IHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWHGYRVVYKR 69
           I FV+++++QG+ R+ ++Y      ER  +  E+    L R    C+FV+   Y+VVY+R
Sbjct: 3   IRFVVMVNKQGQTRVAQYYEHLSVDERRALEGEIVRKCLARTDHQCSFVEHRNYKVVYRR 62

Query: 70  YASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYFILDEVLIAG 129
           YASL+F + +D  +NEL  L+ IH  VE +DR+FGNVCELD++F+  K +F+L+E+++ G
Sbjct: 63  YASLFFLVGVDNDENELAILEFIHLLVETMDRHFGNVCELDIMFHLEKVHFMLEEMVMNG 122

Query: 130 ELQESNKKAVL 140
            + E++K+ +L
Sbjct: 123 CIVETSKQNIL 133



>Os03g0228400 Similar to Adapter-related protein complex 3 sigma 2 subunit
           (Sigma-adaptin 3b) (AP-3 complex sigma-3B subunit)
           (Sigma-3B-adaptin)
          Length = 166

 Score =  113 bits (282), Expect = 8e-26,   Method: Compositional matrix adjust.
 Identities = 55/138 (39%), Positives = 89/138 (64%), Gaps = 5/138 (3%)

Query: 9   MIHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWH-----GY 63
           MI  V+++S QGK RL K+Y+  P ++   +++ V  L+  R   + NFV        G 
Sbjct: 1   MIQAVMVMSTQGKPRLLKFYSYQPPEKHQDLVRGVFQLLSARPDSVSNFVKVDAIFGPGA 60

Query: 64  RVVYKRYASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNVCELDLIFNFHKAYFILD 123
           ++VYK  A+LYF    D+++NEL  L ++  FVE LDR F NVCELD++FNF+K + ILD
Sbjct: 61  KLVYKHLATLYFVFVFDSSENELAVLDLVQVFVETLDRCFKNVCELDIVFNFNKLHTILD 120

Query: 124 EVLIAGELQESNKKAVLR 141
           E+++ G++ E++ + ++R
Sbjct: 121 EMILGGQVIETSSEQIMR 138



>Os08g0395300 Clathrin adaptor complex, small chain family protein
          Length = 100

 Score = 93.6 bits (231), Expect = 6e-20,   Method: Compositional matrix adjust.
 Identities = 43/97 (44%), Positives = 63/97 (64%)

Query: 10  IHFVLLISRQGKVRLTKWYNPYPQKERSKVIKEVSTLVLTRGPKMCNFVDWHGYRVVYKR 69
           I FVL +++QG+ RL ++Y      ER  +  E+    L R  + C+FV+   Y+VVY+R
Sbjct: 3   IRFVLFVNKQGQTRLAQYYEHLSIDERRALEGEIVRKCLARTDQQCSFVEHRNYKVVYRR 62

Query: 70  YASLYFCMCIDAADNELETLQIIHHFVEILDRYFGNV 106
           YASL+F + +D  +NEL  L+ IH FVE +DR+FGNV
Sbjct: 63  YASLFFLVGVDNDENELAILEFIHLFVETMDRHFGNV 99



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.139    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,327,680
Number of extensions: 194319
Number of successful extensions: 545
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 545
Number of HSP's successfully gapped: 6
Length of query: 169
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 75
Effective length of database: 12,127,685
Effective search space: 909576375
Effective search space used: 909576375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 152 (63.2 bits)