BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0288000 Os03g0288000|AK103162
(72 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0288000 Similar to Metallothionein 109 4e-25
Os11g0704500 Metallothionein-like protein type 1 93 3e-20
Os12g0570700 Similar to Metallothionein-like protein type 1 80 4e-16
Os12g0571000 Plant metallothionein, family 15 protein 75 9e-15
Os12g0567800 Plant metallothionein, family 15 protein 72 6e-14
Os12g0571100 Similar to Metallothionein-like protein type 1 72 1e-13
>Os03g0288000 Similar to Metallothionein
Length = 72
Score = 109 bits (273), Expect = 4e-25, Method: Compositional matrix adjust.
Identities = 54/54 (100%), Positives = 54/54 (100%)
Query: 19 KMYPDLEEKSSSAQATVVLGVAPEKAHFEAAAESGETAHGCGCGSSCKCNPCNC 72
KMYPDLEEKSSSAQATVVLGVAPEKAHFEAAAESGETAHGCGCGSSCKCNPCNC
Sbjct: 19 KMYPDLEEKSSSAQATVVLGVAPEKAHFEAAAESGETAHGCGCGSSCKCNPCNC 72
>Os11g0704500 Metallothionein-like protein type 1
Length = 74
Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust.
Identities = 47/56 (83%), Positives = 49/56 (87%), Gaps = 2/56 (3%)
Query: 19 KMYPDLEEKSSSAQATVVLGVAPEKA--HFEAAAESGETAHGCGCGSSCKCNPCNC 72
K YPDLEEKSSS +ATVVLGVAPEK FEAAAESGETAHGC CGSSC+CNPCNC
Sbjct: 19 KKYPDLEEKSSSTKATVVLGVAPEKKAQQFEAAAESGETAHGCSCGSSCRCNPCNC 74
>Os12g0570700 Similar to Metallothionein-like protein type 1
Length = 78
Score = 80.1 bits (196), Expect = 4e-16, Method: Compositional matrix adjust.
Identities = 40/57 (70%), Positives = 44/57 (77%), Gaps = 3/57 (5%)
Query: 19 KMYPDLEEKSSSAQATVVLGVAPEKAHFEA---AAESGETAHGCGCGSSCKCNPCNC 72
MYPDL EK+++ AT+VLGVAP K FE AAESGE AHGC CGSSCKCNPCNC
Sbjct: 22 NMYPDLAEKTTNTSATMVLGVAPAKEQFEGVGKAAESGEAAHGCSCGSSCKCNPCNC 78
>Os12g0571000 Plant metallothionein, family 15 protein
Length = 65
Score = 75.5 bits (184), Expect = 9e-15, Method: Compositional matrix adjust.
Identities = 40/60 (66%), Positives = 45/60 (75%), Gaps = 6/60 (10%)
Query: 19 KMYPDLEEKSSSAQA--TVVLGVAPEK----AHFEAAAESGETAHGCGCGSSCKCNPCNC 72
KMYPDL EK+++ T++LGVAPEK A FEAAA SGE HGC CGSSCKCNPCNC
Sbjct: 6 KMYPDLAEKNTTITTSDTMILGVAPEKGRGEAVFEAAAGSGEADHGCSCGSSCKCNPCNC 65
>Os12g0567800 Plant metallothionein, family 15 protein
Length = 78
Score = 72.4 bits (176), Expect = 6e-14, Method: Compositional matrix adjust.
Identities = 41/59 (69%), Positives = 42/59 (71%), Gaps = 5/59 (8%)
Query: 19 KMYPDLEEKSSSAQATV--VLGVAPEKAHFEA---AAESGETAHGCGCGSSCKCNPCNC 72
KMYPDL EK + T VLGVAPEK FE AAESGE AHGC CGSSCKCNPCNC
Sbjct: 20 KMYPDLAEKITITTTTTTTVLGVAPEKGQFEGVGKAAESGEGAHGCSCGSSCKCNPCNC 78
>Os12g0571100 Similar to Metallothionein-like protein type 1
Length = 78
Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust.
Identities = 41/59 (69%), Positives = 45/59 (76%), Gaps = 5/59 (8%)
Query: 19 KMYPDLEEK--SSSAQATVVLGVAPEKAHFEA---AAESGETAHGCGCGSSCKCNPCNC 72
KMYPDL EK +++ AT VLGVAPEK H E AAESGE AHGC CGSSC+CNPCNC
Sbjct: 20 KMYPDLAEKITTTTTTATTVLGVAPEKGHSEGVGKAAESGEAAHGCSCGSSCRCNPCNC 78
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.313 0.125 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,051,035
Number of extensions: 57400
Number of successful extensions: 286
Number of sequences better than 1.0e-10: 8
Number of HSP's gapped: 277
Number of HSP's successfully gapped: 8
Length of query: 72
Length of database: 17,035,801
Length adjustment: 43
Effective length of query: 29
Effective length of database: 14,790,599
Effective search space: 428927371
Effective search space used: 428927371
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 149 (62.0 bits)