BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0722700 Os02g0722700|J100047H11
(52 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0722700 Conserved hypothetical protein 105 1e-23
Os07g0227400 Similar to RNA binding/signal transduction pro... 79 8e-16
Os01g0818300 KH domain containing protein 77 3e-15
Os01g0886300 KH domain containing protein 77 3e-15
Os03g0815700 KH domain containing protein 76 6e-15
Os05g0419500 KH domain containing protein 73 4e-14
Os05g0481500 KH domain containing protein 71 2e-13
>Os02g0722700 Conserved hypothetical protein
Length = 52
Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 52/52 (100%), Positives = 52/52 (100%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPVVLCF 52
EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPVVLCF
Sbjct: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPVVLCF 52
>Os07g0227400 Similar to RNA binding/signal transduction protein QkI-4
Length = 286
Score = 79.0 bits (193), Expect = 8e-16, Method: Composition-based stats.
Identities = 34/48 (70%), Positives = 44/48 (91%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
E+ ++GKPGYEHLN+PLHIL+EAELP IIDTRL QA++I+++LLKPV
Sbjct: 192 EEKLKGKPGYEHLNDPLHILIEAELPANIIDTRLRQAQEIMDELLKPV 239
>Os01g0818300 KH domain containing protein
Length = 283
Score = 77.0 bits (188), Expect = 3e-15, Method: Composition-based stats.
Identities = 33/48 (68%), Positives = 44/48 (91%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
E+ ++G+PGYEHL++P HIL+EAELP ++IDTRL QA++ILEDLLKPV
Sbjct: 190 EEQLKGRPGYEHLSDPTHILIEAELPADVIDTRLAQAQEILEDLLKPV 237
>Os01g0886300 KH domain containing protein
Length = 290
Score = 77.0 bits (188), Expect = 3e-15, Method: Composition-based stats.
Identities = 37/48 (77%), Positives = 41/48 (85%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
ED +R KPGYEHLNEPLH+LVEAE P +IIDTRL QA ILEDLLKP+
Sbjct: 193 EDKLRDKPGYEHLNEPLHVLVEAEFPADIIDTRLNQAVTILEDLLKPI 240
>Os03g0815700 KH domain containing protein
Length = 281
Score = 75.9 bits (185), Expect = 6e-15, Method: Composition-based stats.
Identities = 32/48 (66%), Positives = 41/48 (85%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
ED +RGKPGYEHL++PLHIL+EAE P IID RL A++++E+LLKPV
Sbjct: 187 EDKLRGKPGYEHLSDPLHILIEAEFPASIIDARLRHAQEVIEELLKPV 234
>Os05g0419500 KH domain containing protein
Length = 291
Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 35/48 (72%), Positives = 40/48 (83%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
ED +R KPGYEHLN+PLH+LVEAE P +I+D RL QA ILEDLLKPV
Sbjct: 192 EDKLRDKPGYEHLNDPLHVLVEAEFPSDIVDVRLNQAVAILEDLLKPV 239
>Os05g0481500 KH domain containing protein
Length = 282
Score = 71.2 bits (173), Expect = 2e-13, Method: Composition-based stats.
Identities = 30/48 (62%), Positives = 42/48 (87%)
Query: 1 EDMMRGKPGYEHLNEPLHILVEAELPVEIIDTRLIQARDILEDLLKPV 48
E+ ++G+ GYEHL++PLHIL+EAELP +ID RL +A++ILE+LLKPV
Sbjct: 190 EEQLKGRAGYEHLDDPLHILIEAELPANVIDARLAKAQEILEELLKPV 237
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.324 0.146 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,668,785
Number of extensions: 44913
Number of successful extensions: 91
Number of sequences better than 1.0e-10: 7
Number of HSP's gapped: 91
Number of HSP's successfully gapped: 7
Length of query: 52
Length of database: 17,035,801
Length adjustment: 25
Effective length of query: 27
Effective length of database: 15,730,451
Effective search space: 424722177
Effective search space used: 424722177
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)