BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0687200 Os02g0687200|AK059947
(106 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os02g0687200 Protein of unknown function DUF581 family protein 177 1e-45
Os04g0586100 Protein of unknown function DUF581 family protein 77 2e-15
Os04g0586200 Similar to Senescence-associated protein SAG102 76 5e-15
Os04g0585700 Protein of unknown function DUF581 family protein 71 2e-13
Os02g0686700 Protein of unknown function DUF581 family protein 71 2e-13
Os04g0586000 Protein of unknown function DUF581 family protein 65 1e-11
AK060089 65 1e-11
Os02g0686900 62 8e-11
>Os02g0687200 Protein of unknown function DUF581 family protein
Length = 106
Score = 177 bits (450), Expect = 1e-45, Method: Compositional matrix adjust.
Identities = 86/96 (89%), Positives = 86/96 (89%)
Query: 11 FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAA 70
FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAA
Sbjct: 11 FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAA 70
Query: 71 VRVSATXXXXXXXXXXQRHRLDAGSVAVAANVPVLS 106
VRVSAT QRHRLDAGSVAVAANVPVLS
Sbjct: 71 VRVSATNARERAARNEQRHRLDAGSVAVAANVPVLS 106
>Os04g0586100 Protein of unknown function DUF581 family protein
Length = 97
Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 37/62 (59%), Positives = 45/62 (72%), Gaps = 4/62 (6%)
Query: 11 FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAA 70
FFD EP+ GE HA+DAC+LC K LTR+ DIFMY+G+TPFCSEECR QM++D
Sbjct: 10 FFDAEPV--GEQ--GRHALDACALCTKQLTRDSDIFMYKGDTPFCSEECRYEQMQLDAVY 65
Query: 71 VR 72
R
Sbjct: 66 AR 67
>Os04g0586200 Similar to Senescence-associated protein SAG102
Length = 104
Score = 75.9 bits (185), Expect = 5e-15, Method: Compositional matrix adjust.
Identities = 37/57 (64%), Positives = 43/57 (75%), Gaps = 4/57 (7%)
Query: 11 FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMD 67
FFD EP+ GE HA+DAC+LC KPL R+ DIFMYRG+TPFCSEECR QM +D
Sbjct: 10 FFDAEPV--GET--GRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMHLD 62
>Os04g0585700 Protein of unknown function DUF581 family protein
Length = 150
Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 30/47 (63%), Positives = 36/47 (76%)
Query: 26 GHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAAVR 72
GH ++AC LCRKPL N DI+MYRG+ PFCSEECR Q+EMD+ R
Sbjct: 67 GHFLEACFLCRKPLASNRDIYMYRGDIPFCSEECRREQIEMDDEMER 113
>Os02g0686700 Protein of unknown function DUF581 family protein
Length = 92
Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 33/47 (70%), Positives = 35/47 (74%)
Query: 27 HAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAAVRV 73
H +DAC LCRK L N DIFMYRG+TPFCSEECR QME D AA R
Sbjct: 8 HFLDACFLCRKRLAGNRDIFMYRGDTPFCSEECRREQMEADAAAERT 54
>Os04g0586000 Protein of unknown function DUF581 family protein
Length = 127
Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 28/43 (65%), Positives = 33/43 (76%)
Query: 27 HAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEA 69
H +D C CR+PL N DIFMYRG+ PFCSEECR Q+E+DEA
Sbjct: 54 HYLDICFRCRRPLGGNRDIFMYRGDMPFCSEECRQEQIEIDEA 96
>AK060089
Length = 127
Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 28/43 (65%), Positives = 33/43 (76%)
Query: 27 HAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEA 69
H +D C CR+PL N DIFMYRG+ PFCSEECR Q+E+DEA
Sbjct: 54 HYLDICFRCRRPLGGNRDIFMYRGDMPFCSEECRQEQIEIDEA 96
>Os02g0686900
Length = 125
Score = 62.0 bits (149), Expect = 8e-11, Method: Compositional matrix adjust.
Identities = 27/44 (61%), Positives = 34/44 (77%)
Query: 27 HAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMDEAA 70
H +DAC LC + L N DIFMYRG+TPFCSEECR Q++ D+A+
Sbjct: 48 HYLDACFLCGRMLAGNKDIFMYRGDTPFCSEECRQRQIDADDAS 91
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.324 0.135 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,274,486
Number of extensions: 108408
Number of successful extensions: 247
Number of sequences better than 1.0e-10: 8
Number of HSP's gapped: 249
Number of HSP's successfully gapped: 8
Length of query: 106
Length of database: 17,035,801
Length adjustment: 74
Effective length of query: 32
Effective length of database: 13,171,965
Effective search space: 421502880
Effective search space used: 421502880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)