BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0141900 Os12g0141900|AK121490
         (101 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0141900  Conserved hypothetical protein                      186   3e-48
Os02g0750600  Ubiquitin domain containing protein                 127   2e-30
Os06g0224100  Conserved hypothetical protein                       93   4e-20
Os03g0426800  Ubiquitin domain containing protein                  87   2e-18
Os04g0393300  Conserved hypothetical protein                       78   2e-15

>Os12g0141900 Conserved hypothetical protein
          Length = 101

 Score =  186 bits (473), Expect = 3e-48,   Method: Compositional matrix adjust.
 Identities = 92/101 (91%), Positives = 92/101 (91%)

Query: 1   RPWRPLHTPACAPAFSTTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLV 60
           RPWRPLHTPACAPAFSTTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLV
Sbjct: 1   RPWRPLHTPACAPAFSTTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLV 60

Query: 61  ESRVRVEVPGGVITMHVVVHPPQSDXXXXXXXXXQRTLWAE 101
           ESRVRVEVPGGVITMHVVVHPPQSD         QRTLWAE
Sbjct: 61  ESRVRVEVPGGVITMHVVVHPPQSDKNKRRRNKNQRTLWAE 101



>Os02g0750600 Ubiquitin domain containing protein
          Length = 119

 Score =  127 bits (319), Expect = 2e-30,   Method: Compositional matrix adjust.
 Identities = 66/71 (92%), Positives = 66/71 (92%), Gaps = 1/71 (1%)

Query: 16 STTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLVESRVRV-EVPGGVIT 74
          STTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTL ESRV V EVPGGVIT
Sbjct: 28 STTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLAESRVPVGEVPGGVIT 87

Query: 75 MHVVVHPPQSD 85
          MHVVV PPQ D
Sbjct: 88 MHVVVRPPQPD 98



>Os06g0224100 Conserved hypothetical protein
          Length = 126

 Score = 93.2 bits (230), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 46/66 (69%), Positives = 55/66 (83%), Gaps = 1/66 (1%)

Query: 16 STTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLVESR-VRVEVPGGVIT 74
          +TTV+ALKEF+LARWPQ KEI P+TVND+ +INAG++LENNRTL ESR +  E P G IT
Sbjct: 34 ATTVTALKEFVLARWPQGKEIAPRTVNDVTIINAGQVLENNRTLAESRNLAAESPEGPIT 93

Query: 75 MHVVVH 80
          MHVVV 
Sbjct: 94 MHVVVR 99



>Os03g0426800 Ubiquitin domain containing protein
          Length = 119

 Score = 87.0 bits (214), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 42/70 (60%), Positives = 54/70 (77%)

Query: 16 STTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLVESRVRVEVPGGVITM 75
          ++TV+ LKE I+A+WP+DKE  P+TVNDLKLINAG+ILENN+TL E +  +    G+ TM
Sbjct: 29 ASTVATLKESIVAQWPKDKEKGPRTVNDLKLINAGKILENNKTLSECKSPICDFSGLTTM 88

Query: 76 HVVVHPPQSD 85
          HVVV  P SD
Sbjct: 89 HVVVRAPTSD 98



>Os04g0393300 Conserved hypothetical protein
          Length = 135

 Score = 77.8 bits (190), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 36/72 (50%), Positives = 52/72 (72%), Gaps = 1/72 (1%)

Query: 14  AFSTTVSALKEFILARWPQDKEITPKTVNDLKLINAGRILENNRTLVESRVRV-EVPGGV 72
           A +TTV+ALK+ ++A WP+DK I PKT ND+KLI+ G+ILEN++ + + R    ++P   
Sbjct: 42  AVATTVAALKDRVVADWPKDKTIVPKTANDVKLISGGKILENDKNIAQCRAPFGDLPSTA 101

Query: 73  ITMHVVVHPPQS 84
           ITMHVVV P  +
Sbjct: 102 ITMHVVVQPSSA 113



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.133    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,289,337
Number of extensions: 100432
Number of successful extensions: 282
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 279
Number of HSP's successfully gapped: 5
Length of query: 101
Length of database: 17,035,801
Length adjustment: 69
Effective length of query: 32
Effective length of database: 13,433,035
Effective search space: 429857120
Effective search space used: 429857120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)