BLAST Search Results

BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0497000 Os11g0497000|AK111924
         (328 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0497000  Similar to Ubiquitin activating enzyme-like pr...   641   0.0  
Os11g0106400  Similar to Ubiquitin-activating enzyme E1 2          91   2e-18
Os03g0820100  Similar to Auxin-resistance protein AXR1             87   2e-17
Os03g0294900  Similar to Ubiquitin-activating enzyme E1 3          86   4e-17
Os07g0692900  Similar to Ubiquitin-activating enzyme E1 3          82   7e-16

>Os11g0497000 Similar to Ubiquitin activating enzyme-like protein (SUMO
           activating enzyme 1a)
          Length = 328

 Score =  641 bits (1654), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 310/310 (100%), Positives = 310/310 (100%)

Query: 19  YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA 78
           YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA
Sbjct: 19  YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA 78

Query: 79  NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP 138
           NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP
Sbjct: 79  NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP 138

Query: 139 IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGEPKPKELAYPSLQ 198
           IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGEPKPKELAYPSLQ
Sbjct: 139 IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGEPKPKELAYPSLQ 198

Query: 199 EAISVPWKNLPRKTTKLYFAMRVLENYESSEGRNACEASLSDRPAVLALRKDMCDKMSLS 258
           EAISVPWKNLPRKTTKLYFAMRVLENYESSEGRNACEASLSDRPAVLALRKDMCDKMSLS
Sbjct: 199 EAISVPWKNLPRKTTKLYFAMRVLENYESSEGRNACEASLSDRPAVLALRKDMCDKMSLS 258

Query: 259 ESQIPTALLERLLAAGKKQHPPVCAILGGILGQEVIKSISGKGDPIKNFFYYDAADGKGI 318
           ESQIPTALLERLLAAGKKQHPPVCAILGGILGQEVIKSISGKGDPIKNFFYYDAADGKGI
Sbjct: 259 ESQIPTALLERLLAAGKKQHPPVCAILGGILGQEVIKSISGKGDPIKNFFYYDAADGKGI 318

Query: 319 AEDIPPLSSD 328
           AEDIPPLSSD
Sbjct: 319 AEDIPPLSSD 328



>Os11g0106400 Similar to Ubiquitin-activating enzyme E1 2
          Length = 1048

 Score = 90.5 bits (223), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 55/168 (32%), Positives = 86/168 (51%), Gaps = 6/168 (3%)

Query: 19  YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA 78
           + RQ+ V+G +  KRL  ++VLV G+NG   E  KN+VLAGV S++L DD  V   DL++
Sbjct: 46  HSRQLAVYGRETMKRLFASNVLVSGLNGLGAEIAKNLVLAGVKSVNLHDDDNVELWDLSS 105

Query: 79  NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP 138
           NF +   +    G++RA+ C + L++ N  V ++   GD   +  E L  F  +V +   
Sbjct: 106 NFFLTEKDV---GQNRAQTCVQKLQELNNAVIISTITGD---LTKEQLSNFQAVVFTDIS 159

Query: 139 IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGE 186
           ++  +  +  C      IAF   E +   G +F D      V  V GE
Sbjct: 160 LEKAVEFDSYCHNHQPPIAFIKSEIRGLFGSVFCDFGPEFTVLDVDGE 207



>Os03g0820100 Similar to Auxin-resistance protein AXR1
          Length = 531

 Score = 87.0 bits (214), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 56/168 (33%), Positives = 92/168 (54%), Gaps = 12/168 (7%)

Query: 19  YDRQIRVWGVDAQKRLSKAHV--LVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDL 76
           YDRQ+R+WG   Q  L KA +  L CG  G  TE  KN+VL GVGS++++D   V + D+
Sbjct: 16  YDRQLRIWGDQGQAALEKASICLLTCGPTG--TEAMKNLVLGGVGSVTVVDGSKVEQSDM 73

Query: 77  NANFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVA-VEKGDPSLIDGE--FLDKFDIIV 133
             NFL+   ++   G+SRA+  C  L++ N  V    VE+   +LID    F  +F +++
Sbjct: 74  GNNFLL---DAECLGQSRAKSVCSFLQELNDAVNAKFVEESPLALIDTNPSFFSQFTVVI 130

Query: 134 VSCAPIKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQ 181
            +  P ++ L ++D CRK   +I   A       G + + ++ H+ ++
Sbjct: 131 ATQLPERSLLKLDDICRK--ANIVLVAARSYGLTGLVRISVKEHNVIE 176



>Os03g0294900 Similar to Ubiquitin-activating enzyme E1 3
          Length = 1064

 Score = 85.9 bits (211), Expect = 4e-17,   Method: Compositional matrix adjust.
 Identities = 54/168 (32%), Positives = 81/168 (48%), Gaps = 6/168 (3%)

Query: 19  YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA 78
           + RQ+ V+G +  +RL  ++VLV G+NG   E  KN+ LAGV S++L D   V   DL+ 
Sbjct: 62  HSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSG 121

Query: 79  NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP 138
           NF +  D+    G++RA  C   L++ N  V ++    D   +  E L KF  +V +   
Sbjct: 122 NFFLSEDDI---GKNRAVACTAKLQELNNAVLISTLTED---LTNEHLSKFQAVVFTDIS 175

Query: 139 IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGE 186
           +      +D CR     I+F   E     G +F D      V  V GE
Sbjct: 176 LDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGE 223



>Os07g0692900 Similar to Ubiquitin-activating enzyme E1 3
          Length = 1045

 Score = 81.6 bits (200), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 53/168 (31%), Positives = 81/168 (48%), Gaps = 6/168 (3%)

Query: 19  YDRQIRVWGVDAQKRLSKAHVLVCGMNGTTTEFCKNIVLAGVGSLSLMDDHLVTEDDLNA 78
           + RQ+ V+G +  +RL  +HVLV G+NG   E  KN+ LAGV S++L D   V   DL+A
Sbjct: 43  HSRQLAVYGRETMRRLFASHVLVSGLNGLGAEIAKNLALAGVKSVTLHDVKNVEMWDLSA 102

Query: 79  NFLIPHDESIYGGRSRAEVCCESLKDFNPMVRVAVEKGDPSLIDGEFLDKFDIIVVSCAP 138
           NF +  ++    G++RA  C   L++ N  V V+    +   +  + L KF  +V +   
Sbjct: 103 NFFLSENDI---GKNRAAACVSKLQELNNAVLVSALTEE---LTTDHLSKFQAVVFTDIG 156

Query: 139 IKTKLLINDNCRKRSKHIAFYAIECKDSCGEIFVDLQNHSYVQKVGGE 186
           +      +D C      I+F   E     G +F D      V  V GE
Sbjct: 157 LDKAYEFDDYCHSHCPPISFIKAEVCGLFGTVFCDFGPEFTVLDVDGE 204



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,996,364
Number of extensions: 459882
Number of successful extensions: 820
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 817
Number of HSP's successfully gapped: 5
Length of query: 328
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 227
Effective length of database: 11,762,187
Effective search space: 2670016449
Effective search space used: 2670016449
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)