BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0516200 Os10g0516200|009-037-B10
         (287 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0516200  Protein kinase-like domain containing protein       289   2e-78
Os03g0113000  Protein kinase-like domain containing protein       140   8e-34
Os04g0540900  Protein kinase-like domain containing protein       109   2e-24
Os02g0648100  Protein kinase-like domain containing protein       104   7e-23
Os02g0106900  Protein kinase-like domain containing protein        78   9e-15

>Os10g0516200 Protein kinase-like domain containing protein
          Length = 287

 Score =  289 bits (740), Expect = 2e-78,   Method: Compositional matrix adjust.
 Identities = 164/271 (60%), Positives = 164/271 (60%)

Query: 1   MWQRYRFLCCGCGGNMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRRLS 60
           MWQRYRFLCCGCGGNM                                        RRLS
Sbjct: 1   MWQRYRFLCCGCGGNMAASAAGDRGCDDDADCGGGSGGGGFGEEEEGGKGGVAGAARRLS 60

Query: 61  WAQVEAMTGGFTSAVVGEGGFSTVYLARVAGALAAVKVHRSSERLHRVFRQELDALLRVR 120
           WAQVEAMTGGFTSAVVGEGGFSTVYLARVAGALAAVKVHRSSERLHRVFRQELDALLRVR
Sbjct: 61  WAQVEAMTGGFTSAVVGEGGFSTVYLARVAGALAAVKVHRSSERLHRVFRQELDALLRVR 120

Query: 121 HPHIVRLLAFCEQQGGLGAPVLARASPRAFARAGCVPPRRAEEFFGFFLSLQRKACWCWX 180
           HPHIVRLLAFCEQQGGLGAPVLARASPRAFARAGCVPPRRAEEFFGFFLSLQRKACWCW 
Sbjct: 121 HPHIVRLLAFCEQQGGLGAPVLARASPRAFARAGCVPPRRAEEFFGFFLSLQRKACWCWS 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASPRWXXXXXXXXXXXX 240
                                                      ASPRW            
Sbjct: 181 SRRTGTCTSGCTAAGRPRGRCRGRGARRWRCRWRGRWSTCTTGASPRWCTATSRRRTCSS 240

Query: 241 XXXXXXXXXXXGRRGWGSPLPSAPGPPRTPC 271
                      GRRGWGSPLPSAPGPPRTPC
Sbjct: 241 TRPCPPSSATSGRRGWGSPLPSAPGPPRTPC 271



>Os03g0113000 Protein kinase-like domain containing protein
          Length = 343

 Score =  140 bits (354), Expect = 8e-34,   Method: Compositional matrix adjust.
 Identities = 70/78 (89%), Positives = 74/78 (94%)

Query: 57  RRLSWAQVEAMTGGFTSAVVGEGGFSTVYLARVAGALAAVKVHRSSERLHRVFRQELDAL 116
           R+LSWAQVEAMT GFTSAVVGEGGFSTVYL RVAG+LAAVKVHRSSERL R FRQELDAL
Sbjct: 49  RQLSWAQVEAMTRGFTSAVVGEGGFSTVYLGRVAGSLAAVKVHRSSERLQRAFRQELDAL 108

Query: 117 LRVRHPHIVRLLAFCEQQ 134
           LRVRHPHIVRLLAFC+Q+
Sbjct: 109 LRVRHPHIVRLLAFCDQR 126



>Os04g0540900 Protein kinase-like domain containing protein
          Length = 361

 Score =  109 bits (273), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 52/79 (65%), Positives = 67/79 (84%), Gaps = 1/79 (1%)

Query: 57  RRLSWAQVEAMTGGFTSAVVGEGGFSTVYLARVAGA-LAAVKVHRSSERLHRVFRQELDA 115
           R+L+WA VE++TGGF+S V+G GGFSTVYLA ++ + L AVKVH SSERLHR FRQEL+ 
Sbjct: 54  RQLAWADVESVTGGFSSRVIGHGGFSTVYLASLSSSRLGAVKVHCSSERLHRAFRQELEV 113

Query: 116 LLRVRHPHIVRLLAFCEQQ 134
           LL +RHPHIVRLL +C+++
Sbjct: 114 LLSLRHPHIVRLLGYCDER 132



>Os02g0648100 Protein kinase-like domain containing protein
          Length = 365

 Score =  104 bits (260), Expect = 7e-23,   Method: Compositional matrix adjust.
 Identities = 49/79 (62%), Positives = 64/79 (81%), Gaps = 1/79 (1%)

Query: 57  RRLSWAQVEAMTGGFTSAVVGEGGFSTVYLARVAGA-LAAVKVHRSSERLHRVFRQELDA 115
           RR  WA++E++TGGF+S V+G+GGFSTVYLA +  + L AVKV RSSERLHR F QE D 
Sbjct: 54  RRFGWAEIESVTGGFSSRVIGQGGFSTVYLASLTSSRLGAVKVQRSSERLHRAFCQERDV 113

Query: 116 LLRVRHPHIVRLLAFCEQQ 134
           LL +RHPH+VRLL +C+++
Sbjct: 114 LLSLRHPHVVRLLGYCDER 132



>Os02g0106900 Protein kinase-like domain containing protein
          Length = 406

 Score = 77.8 bits (190), Expect = 9e-15,   Method: Compositional matrix adjust.
 Identities = 57/112 (50%), Positives = 65/112 (58%), Gaps = 11/112 (9%)

Query: 58  RLSWAQVEAMTGGFT-SAVVGEGGFSTVYLAR-----VAGALAAVKVHR--SSERLHRVF 109
           +L+W +VEA+TGGF  +AVVG GG STVYLA      V     AVKVHR    ER  R F
Sbjct: 93  KLTWREVEALTGGFDEAAVVGRGGSSTVYLASSCRVAVVSPPVAVKVHRWCGGERWLRAF 152

Query: 110 RQELDALLRVRHPHIVRLLAFCEQQGGLGAPVLARASPRAFA---RAGCVPP 158
           RQELD L R+RHPHIV LLA+ +     GA VL   S    A     G  PP
Sbjct: 153 RQELDLLRRLRHPHIVSLLAYSDDHEEGGALVLEYLSGGTLADRLHGGASPP 204



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.140    0.496 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,869,403
Number of extensions: 220040
Number of successful extensions: 2154
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 2185
Number of HSP's successfully gapped: 5
Length of query: 287
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 187
Effective length of database: 11,814,401
Effective search space: 2209292987
Effective search space used: 2209292987
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 155 (64.3 bits)