BLAST Search Results

BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0505600 Os09g0505600|AK103136
         (221 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0505600  Proteasome subunit beta type 1 (EC 3.4.25.1) (...   459   e-130
Os08g0529100  Similar to Proteasome subunit beta type 1 (EC ...   402   e-112
Os06g0643100  Proteasome subunit beta type 3 (EC 3.4.25.1) (...    80   1e-15
Os06g0139900  Similar to Beta 1 subunit of 20S proteasome          68   5e-12
Os02g0770000  Beta 1 subunit of 20S proteasome                     67   7e-12

>Os09g0505600 Proteasome subunit beta type 1 (EC 3.4.25.1) (20S proteasome alpha
           subunit F) (20S proteasome subunit beta-6)
          Length = 221

 Score =  459 bits (1182), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 221/221 (100%), Positives = 221/221 (100%)

Query: 1   MSRRGDWVYENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSG 60
           MSRRGDWVYENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSG
Sbjct: 1   MSRRGDWVYENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSG 60

Query: 61  FQGDIKALHKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSE 120
           FQGDIKALHKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSE
Sbjct: 61  FQGDIKALHKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSE 120

Query: 121 GKGCVFTYDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAV 180
           GKGCVFTYDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAV
Sbjct: 121 GKGCVFTYDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAV 180

Query: 181 DLVKDVFASATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221
           DLVKDVFASATERDIYTGDKLEIVVINKAGTKREYIDLRKD
Sbjct: 181 DLVKDVFASATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221



>Os08g0529100 Similar to Proteasome subunit beta type 1 (EC 3.4.25.1) (20S
           proteasome alpha subunit F) (20S proteasome subunit
           beta-6)
          Length = 219

 Score =  402 bits (1032), Expect = e-112,   Method: Compositional matrix adjust.
 Identities = 189/214 (88%), Positives = 205/214 (95%)

Query: 8   VYENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKA 67
           VYENNGGTCVA+AGADYCVVAADTRLSVGY+IL+RD SKI  LADKC LASSGFQGD+KA
Sbjct: 6   VYENNGGTCVAVAGADYCVVAADTRLSVGYSILSRDSSKIAHLADKCVLASSGFQGDVKA 65

Query: 68  LHKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFT 127
           L +NL A+ELLY+H HNK+MSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFT
Sbjct: 66  LQRNLTAKELLYEHNHNKKMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFT 125

Query: 128 YDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDVF 187
           YDAVGSYERTGYSAQGTG+AL+MPVLDNQLKSPSPLLLPARDAVTPLS+++AVDLVKDVF
Sbjct: 126 YDAVGSYERTGYSAQGTGAALMMPVLDNQLKSPSPLLLPARDAVTPLSQSDAVDLVKDVF 185

Query: 188 ASATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221
           ASATERDIYTGDKLEIVVIN AG+ RE+I+LRKD
Sbjct: 186 ASATERDIYTGDKLEIVVINSAGSHREFIELRKD 219



>Os06g0643100 Proteasome subunit beta type 3 (EC 3.4.25.1) (20S proteasome alpha
           subunit C) (20S proteasome subunit beta-3)
          Length = 204

 Score = 80.5 bits (197), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 56/215 (26%), Positives = 95/215 (44%), Gaps = 14/215 (6%)

Query: 8   VYENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKA 67
           ++E NG   VA+ G +   +A+D RL V    +  D  ++ ++ DK  +  SG   D + 
Sbjct: 3   IFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTVATDFQRVFKIHDKLYIGLSGLATDAQT 62

Query: 68  LHKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFT 127
           L++ L  R  LYQ +  + M     A L+S  LY KRF PY+   V+ GL  + +  + T
Sbjct: 63  LYQRLVFRHKLYQLREERDMKPQTFASLVSALLYEKRFGPYFCQPVIAGLGEDNEPFICT 122

Query: 128 YDAVGSYERTG-YSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDV 186
            D +G+ E    +   GT S  +    ++  K   P + P           E  + +   
Sbjct: 123 MDCIGAKELAKDFVVSGTASESLYGACESMYK---PNMEP----------EELFETISQA 169

Query: 187 FASATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221
             S+ +RD  +G    ++++     K   I  R D
Sbjct: 170 LQSSVDRDCLSGWGGFVLLVTPTEVKECVIKGRMD 204



>Os06g0139900 Similar to Beta 1 subunit of 20S proteasome
          Length = 246

 Score = 67.8 bits (164), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 55/209 (26%), Positives = 91/209 (43%), Gaps = 26/209 (12%)

Query: 13  GGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKALHKNL 72
           G T V +      V+AAD+R S G  +  R   KI +L D   +  SG   D + + + +
Sbjct: 25  GTTIVGVCYDGGVVLAADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADTQVISEYV 84

Query: 73  AARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVL------GGLDSEGKGCVF 126
                 + HQH  ++  PA  ++ +N +   R   Y   N+L      GG D    G +F
Sbjct: 85  R----YFLHQHTIQLGQPATVKVAANLI---RLLAYQNKNMLQAGMIVGGWDKYEGGQIF 137

Query: 127 TYDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDV 186
           +    G+  R  ++  G+GS+ +  +LD++ K               +S+ EA   V  V
Sbjct: 138 SVPLGGTILRQPFAIGGSGSSYLYGLLDHEWKE-------------GMSQEEAEKFVVKV 184

Query: 187 FASATERDIYTGDKLEIVVINKAGTKREY 215
            + A  RD  +G  +  V IN  G  R++
Sbjct: 185 VSLAIARDGASGGVVRTVTINADGVSRKF 213



>Os02g0770000 Beta 1 subunit of 20S proteasome
          Length = 246

 Score = 67.4 bits (163), Expect = 7e-12,   Method: Compositional matrix adjust.
 Identities = 57/209 (27%), Positives = 91/209 (43%), Gaps = 26/209 (12%)

Query: 13  GGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKALHKNL 72
           G T V +      V+AAD+R S G  +  R   KI +L D   +  SG   D + +   +
Sbjct: 25  GTTIVGVCYDGGVVLAADSRTSTGMYVANRASDKITQLTDNVYICRSGSAADTQVISDYV 84

Query: 73  AARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVL------GGLDSEGKGCVF 126
             R  L  HQH  ++  PA  ++ +N +   R   Y   N+L      GG D    G +F
Sbjct: 85  --RYFL--HQHTIQLGQPATVKVAANLI---RLLAYQNKNMLQAGMIVGGWDKYEGGQIF 137

Query: 127 TYDAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDV 186
           +    G+  R  ++  G+GS+ +  +LD++ K               +S+ EA   V  V
Sbjct: 138 SVPLGGTILRQPFAIGGSGSSYLYGLLDHEWKE-------------GMSQEEAEKFVVKV 184

Query: 187 FASATERDIYTGDKLEIVVINKAGTKREY 215
            + A  RD  +G  +  V IN  G  R++
Sbjct: 185 VSLAIARDGASGGVVRTVTINADGVSRKF 213



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,393,543
Number of extensions: 293582
Number of successful extensions: 656
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 658
Number of HSP's successfully gapped: 5
Length of query: 221
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 124
Effective length of database: 11,971,043
Effective search space: 1484409332
Effective search space used: 1484409332
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)