BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0412600 Os08g0412600|AK099868
         (284 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0412600  RabGAP/TBC domain containing protein                583   e-167
Os08g0337700  RabGAP/TBC domain containing protein                128   5e-30
Os05g0465100  RabGAP/TBC domain containing protein                103   2e-22
Os09g0515800  RabGAP/TBC domain containing protein                 74   1e-13
Os03g0283800  RabGAP/TBC domain containing protein                 72   3e-13

>Os08g0412600 RabGAP/TBC domain containing protein
          Length = 284

 Score =  583 bits (1503), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 284/284 (100%), Positives = 284/284 (100%)

Query: 1   MDKKKAVDDTEPGPAPSRPVDRFGFIKPEQGTSPDGISKSKFIHERERERDGRRIKKWRK 60
           MDKKKAVDDTEPGPAPSRPVDRFGFIKPEQGTSPDGISKSKFIHERERERDGRRIKKWRK
Sbjct: 1   MDKKKAVDDTEPGPAPSRPVDRFGFIKPEQGTSPDGISKSKFIHERERERDGRRIKKWRK 60

Query: 61  MIGVGGSDWKHYVRRNPHVVKRRIRKGIPDCLRGLVWQLISGSRDLLLMNPGVYETLVIY 120
           MIGVGGSDWKHYVRRNPHVVKRRIRKGIPDCLRGLVWQLISGSRDLLLMNPGVYETLVIY
Sbjct: 61  MIGVGGSDWKHYVRRNPHVVKRRIRKGIPDCLRGLVWQLISGSRDLLLMNPGVYETLVIY 120

Query: 121 ETSASELEIIRDISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGL 180
           ETSASELEIIRDISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGL
Sbjct: 121 ETSASELEIIRDISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGL 180

Query: 181 LLLYMSEEDAFWLLVALLKGAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFI 240
           LLLYMSEEDAFWLLVALLKGAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFI
Sbjct: 181 LLLYMSEEDAFWLLVALLKGAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFI 240

Query: 241 EEMINPSMYASQWFITVFSYSFPFHLTLRVWDVFLYEVRILPAI 284
           EEMINPSMYASQWFITVFSYSFPFHLTLRVWDVFLYEVRILPAI
Sbjct: 241 EEMINPSMYASQWFITVFSYSFPFHLTLRVWDVFLYEVRILPAI 284



>Os08g0337700 RabGAP/TBC domain containing protein
          Length = 419

 Score =  128 bits (321), Expect = 5e-30,   Method: Compositional matrix adjust.
 Identities = 85/258 (32%), Positives = 124/258 (48%), Gaps = 33/258 (12%)

Query: 46  RERERDGRRIKKWRKMIGVGGSDW---------------------KHYVRRNPHVVKRRI 84
           RER R+  R+  W  +    G++W                     K  V  N   ++R I
Sbjct: 78  RERMREQGRV--WWALEASKGANWYLQPRISSNGGSEGVISVTSLKLSVLTNTITLRRLI 135

Query: 85  RKGIPDCLRGLVWQLISGSRDLLLMNPGVYETLVIY----ETSASELEIIRDISRTFPSH 140
           RKG+P  LR  VW  +SG+       P  Y   +I     +T+ + L+I  D+ RTFP H
Sbjct: 136 RKGVPPVLRPKVWLSVSGAAKKRSTVPETYYDELIRATEGKTTPATLQIDHDLPRTFPCH 195

Query: 141 IFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGLLLLYM-SEEDAFWLLVALLK 199
            +     G  Q SL  VL  YS  D +VGY QG+ ++A LLLL M +EEDAFW+L  LL+
Sbjct: 196 SWLNSEEG--QASLRRVLVGYSFRDSEVGYCQGLNYVAALLLLVMKTEEDAFWMLAVLLE 253

Query: 200 GAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFIEEMINPSMYASQWFITVFS 259
             +   +   Y   L         F+ L+ ++ P++  H      + S+ A++WF+ +FS
Sbjct: 254 NVL---VNDCYTDNLSGCHVEQRVFKDLLAKKCPRIAAHLEAMGFDVSLVATEWFLCLFS 310

Query: 260 YSFPFHLTLRVWDVFLYE 277
            + P   TLRVWDV   E
Sbjct: 311 KTLPSETTLRVWDVLFNE 328



>Os05g0465100 RabGAP/TBC domain containing protein
          Length = 830

 Score =  103 bits (256), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 68/216 (31%), Positives = 103/216 (47%), Gaps = 29/216 (13%)

Query: 84  IRKGIPDCLRGLVWQL--------ISGSRDLLLMNPGVYETLVIYETSASEL-------- 127
           +  G+P  LRG +WQ         + G  D LL+  G  E      +S S++        
Sbjct: 191 VHGGLPMALRGELWQAFVGIGVRRVKGYYDSLLVAEGELEDTSGSRSSTSDVAGENTEVS 250

Query: 128 ------EIIRDISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGLL 181
                 +I +D+ RTFP H    +    G+ +L  +L AY+ ++  VGY Q M F AGLL
Sbjct: 251 SEKWKGQIEKDLPRTFPGHPALDE---DGRNALRRLLLAYARHNPAVGYCQAMNFFAGLL 307

Query: 182 LLYMSEEDAFWLLVALLKGAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFIE 241
           LL M EE+AFW LV    G +    +G +   +   Q      E+LV E+ P+L  H   
Sbjct: 308 LLLMPEENAFWTLV----GIIDDYFDGYFSEEMIESQVDQLVLEELVREKFPKLANHLDY 363

Query: 242 EMINPSMYASQWFITVFSYSFPFHLTLRVWDVFLYE 277
             +  +     WF+++F+   P+   LRVWDV L++
Sbjct: 364 LGLQVAWVTGPWFLSIFTNVLPWESVLRVWDVLLFD 399



>Os09g0515800 RabGAP/TBC domain containing protein
          Length = 444

 Score = 73.9 bits (180), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 71/303 (23%), Positives = 124/303 (40%), Gaps = 66/303 (21%)

Query: 27  KPEQGTSPDGISKSKFIHERERERDGRRIKKWRKMIGVGGSDWKHYVRRNPHVVKRRIRK 86
            P   +S + +  ++ +    R  D  RI K+   +              P V+  ++R+
Sbjct: 95  NPPSTSSTNSLPDAQGLVSGARATDSARIAKFTNELS------------RPAVILDKLRE 142

Query: 87  ----GIPDCLRGLVWQLISG-------SRDLLLMNPGV--------YETLVIYETSASEL 127
               G+P  +R  +W+L+ G        R+ +L    +        Y  +   E S  E+
Sbjct: 143 LSWSGVPPYMRPNIWRLLLGYAPPNADRREGVLTRKRLEYVECVSQYYDIPDTERSDEEI 202

Query: 128 EIIR----DISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMG-FLAGLLL 182
            ++R    D  RT P   FFQ  H   Q+SL  +L  +++     GYVQG+   L   L+
Sbjct: 203 NMLRQIAVDCPRTVPDVTFFQ--HPQIQKSLERILYTWAIRHPASGYVQGINDLLTPFLV 260

Query: 183 LYMSEE-----------------------DAFWLLVALLKGAVHAPMEGLYQAGLPLVQQ 219
           +++SE                        D +W L   L G     M+  Y    P +Q+
Sbjct: 261 VFLSEHLEGNMDTWSMEKLSPQDVSNIEADCYWCLSKFLDG-----MQDHYTFAQPGIQR 315

Query: 220 YLYQFEKLVLEQMPQLGQHFIEEMINPSMYASQWFITVFSYSFPFHLTLRVWDVFLYEVR 279
            +++ ++LV      + +H  E+ ++   +A +WF  +     PFHL  R+WD +L E  
Sbjct: 316 LVFRLKELVHRIDEPVSKHMEEQGLDFLQFAFRWFNCLMIREIPFHLVTRLWDTYLAEGD 375

Query: 280 ILP 282
            LP
Sbjct: 376 YLP 378



>Os03g0283800 RabGAP/TBC domain containing protein
          Length = 157

 Score = 72.4 bits (176), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 44/133 (33%), Positives = 67/133 (50%), Gaps = 7/133 (5%)

Query: 132 DISRTFPSHIFFQQRHGPGQRSLYNVLKAYSVYDRDVGYVQGMGFLAGLLLLYMSEEDAF 191
           D+ RTFP H    +    G+ +L  +L AY+ ++  VGY Q M F AGL LL+M EE AF
Sbjct: 1   DLPRTFPGHPALDED---GRNALRRLLTAYARHNPSVGYCQAMNFFAGLFLLFMPEEHAF 57

Query: 192 WLLVALLKGAVHAPMEGLYQAGLPLVQQYLYQFEKLVLEQMPQLGQHFIEEMINPSMYAS 251
           W LV    G +    +G Y   +   Q      E++V E+ P+L +H     +  +    
Sbjct: 58  WALV----GVIDEYFDGYYTEEMIESQVDQLVLEEVVRERFPKLAKHMDFLGVQVAWVTG 113

Query: 252 QWFITVFSYSFPF 264
            WF+++F    P+
Sbjct: 114 PWFLSIFINMLPW 126



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.142    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,499,079
Number of extensions: 465613
Number of successful extensions: 940
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 937
Number of HSP's successfully gapped: 5
Length of query: 284
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 184
Effective length of database: 11,814,401
Effective search space: 2173849784
Effective search space used: 2173849784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 155 (64.3 bits)