BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0249700 Os07g0249700|J100085L11
         (60 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0249700  Peptidase M20 family protein                        122   6e-29
Os01g0560000  Similar to Auxin amidohydrolase                      69   5e-13
Os03g0836900  Similar to IAA-amino acid hydrolase 1 (EC 3.5....    68   2e-12
Os01g0706900  Similar to Auxin amidohydrolase                      67   3e-12
Os06g0691400  Similar to IAA-amino acid conjugate hydrolase-...    67   3e-12
Os04g0521800  Peptidase M20 family protein                         66   7e-12

>Os07g0249700 Peptidase M20 family protein
          Length = 60

 Score =  122 bits (306), Expect = 6e-29,   Method: Compositional matrix adjust.
 Identities = 60/60 (100%), Positives = 60/60 (100%)

Query: 1  HFYFLAVLTVWKELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLKVLTIVH 60
          HFYFLAVLTVWKELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLKVLTIVH
Sbjct: 1  HFYFLAVLTVWKELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLKVLTIVH 60



>Os01g0560000 Similar to Auxin amidohydrolase
          Length = 442

 Score = 69.3 bits (168), Expect = 5e-13,   Method: Composition-based stats.
 Identities = 27/43 (62%), Positives = 35/43 (81%)

Query: 12  KELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLK 54
           +E V+WEHKS+  GKMH CGHDAH  MLLG+A++LQ  +D+LK
Sbjct: 117 QESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHRDELK 159



>Os03g0836900 Similar to IAA-amino acid hydrolase 1 (EC 3.5.1.-)
          Length = 414

 Score = 67.8 bits (164), Expect = 2e-12,   Method: Composition-based stats.
 Identities = 29/40 (72%), Positives = 34/40 (85%)

Query: 12  KELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKD 51
           +E+V+WE KS E GKMHACGHDAH  MLL AAKLLQS++D
Sbjct: 92  QEMVEWEFKSLEDGKMHACGHDAHVAMLLVAAKLLQSRRD 131



>Os01g0706900 Similar to Auxin amidohydrolase
          Length = 456

 Score = 66.6 bits (161), Expect = 3e-12,   Method: Composition-based stats.
 Identities = 28/43 (65%), Positives = 37/43 (86%)

Query: 12  KELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLK 54
           +E V WEHKS+ + KMHACGHDAHTTMLLGAA++LQ ++ +L+
Sbjct: 131 QEEVQWEHKSKVAMKMHACGHDAHTTMLLGAARILQERRHELQ 173



>Os06g0691400 Similar to IAA-amino acid conjugate hydrolase-like protein
           (Fragment)
          Length = 510

 Score = 66.6 bits (161), Expect = 3e-12,   Method: Composition-based stats.
 Identities = 27/43 (62%), Positives = 36/43 (83%)

Query: 12  KELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLK 54
           +E V+WEHKS+  GKMHACGHDAH  MLLGAAK+L++++  L+
Sbjct: 180 QEAVEWEHKSKNPGKMHACGHDAHVAMLLGAAKILKAREHHLR 222



>Os04g0521800 Peptidase M20 family protein
          Length = 426

 Score = 65.9 bits (159), Expect = 7e-12,   Method: Compositional matrix adjust.
 Identities = 29/43 (67%), Positives = 35/43 (81%)

Query: 12  KELVDWEHKSEESGKMHACGHDAHTTMLLGAAKLLQSQKDDLK 54
           +ELV+WEHKS+  G MHACGHD HT MLLGAAKLL  +K+ +K
Sbjct: 104 QELVEWEHKSKVDGVMHACGHDVHTAMLLGAAKLLSERKEQIK 146



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.133    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,960,651
Number of extensions: 52504
Number of successful extensions: 152
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 6
Length of query: 60
Length of database: 17,035,801
Length adjustment: 32
Effective length of query: 28
Effective length of database: 15,364,953
Effective search space: 430218684
Effective search space used: 430218684
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)