BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0507300 Os05g0507300|AK060834
         (302 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0507300  Similar to Calreticulin 3 (Fragment)                429   e-120
Os07g0246200  Similar to Calreticulin (Fragment)                  275   4e-74
Os03g0832200  Similar to Calcium-binding protein precursor (...   273   1e-73
Os01g0895600  Similar to Calreticulin 3 (Fragment)                133   2e-31
Os04g0402100  Similar to Calnexin (Fragment)                      125   3e-29

>Os05g0507300 Similar to Calreticulin 3 (Fragment)
          Length = 302

 Score =  429 bits (1103), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 214/235 (91%), Positives = 214/235 (91%)

Query: 1   MSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFY 60
           MSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFY
Sbjct: 1   MSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFY 60

Query: 61  TFILRPDASYSLLVDNREREFGSMYTDWDILPPRKIKESNAKKPKDWDDREYIEDPDEVK 120
           TFILRPDASYSLLVDNREREFGSMYTDWDILPPRKIKESNAKKPKDWDDREYIEDPDEVK
Sbjct: 61  TFILRPDASYSLLVDNREREFGSMYTDWDILPPRKIKESNAKKPKDWDDREYIEDPDEVK 120

Query: 121 PEGYDSXXXXXXXXXXXXXXXXXXXXXGVWKPRMIPNPEYKGRWKRKKIKNPNYKGKWKI 180
           PEGYDS                     GVWKPRMIPNPEYKGRWKRKKIKNPNYKGKWKI
Sbjct: 121 PEGYDSIPKEIPDPKDKKPESWDDDDDGVWKPRMIPNPEYKGRWKRKKIKNPNYKGKWKI 180

Query: 181 PWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVDEV 235
           PWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVDEV
Sbjct: 181 PWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVDEV 235



>Os07g0246200 Similar to Calreticulin (Fragment)
          Length = 424

 Score =  275 bits (702), Expect = 4e-74,   Method: Compositional matrix adjust.
 Identities = 133/235 (56%), Positives = 171/235 (72%)

Query: 1   MSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFY 60
           + G V+QKKFGGDTPYS MFGPDICG  TKK+H I +   +N+ IKKD+ CETD+L+H Y
Sbjct: 123 LGGDVDQKKFGGDTPYSIMFGPDICGYSTKKVHTIFTKNDKNHLIKKDVPCETDQLSHVY 182

Query: 61  TFILRPDASYSLLVDNREREFGSMYTDWDILPPRKIKESNAKKPKDWDDREYIEDPDEVK 120
           T I+ PDA+Y++L+DN E++ GS+Y  WDILPP++IK+  AKKP+DWDD+EYI DP++ K
Sbjct: 183 TLIIHPDATYTILIDNVEKQSGSIYEHWDILPPKQIKDPEAKKPEDWDDKEYIPDPEDKK 242

Query: 121 PEGYDSXXXXXXXXXXXXXXXXXXXXXGVWKPRMIPNPEYKGRWKRKKIKNPNYKGKWKI 180
           PEGYD                      G W    IPNPEYKG WK+KKIKNPNY+GKWK 
Sbjct: 243 PEGYDDIPKEIPDPDAKKPEDWDDEEDGEWTAPTIPNPEYKGPWKQKKIKNPNYQGKWKA 302

Query: 181 PWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVDEV 235
           P IDNP+F+DDP +Y    L+Y+GIE+WQVK+G++FDN LI DDPE A++  +E 
Sbjct: 303 PMIDNPDFKDDPYIYAFDSLKYIGIELWQVKSGTLFDNFLITDDPELAKTFAEET 357



>Os03g0832200 Similar to Calcium-binding protein precursor (Calreticulin)
          Length = 442

 Score =  273 bits (698), Expect = 1e-73,   Method: Compositional matrix adjust.
 Identities = 131/235 (55%), Positives = 169/235 (71%)

Query: 1   MSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFY 60
           + G V+QKKFGG+TPYS MFGPDICG  TKK+H IL+  G+N+ IKKD+ C+TD+LTH Y
Sbjct: 119 LGGDVDQKKFGGETPYSIMFGPDICGYATKKVHAILTKNGKNHLIKKDVPCKTDQLTHVY 178

Query: 61  TFILRPDASYSLLVDNREREFGSMYTDWDILPPRKIKESNAKKPKDWDDREYIEDPDEVK 120
           T I+RPDA YS+L+DN E++ GS+Y DW+I+PP+  ++  AKKP+DWDD EYI DP++ K
Sbjct: 179 TLIIRPDAKYSILIDNTEKQTGSIYDDWNIIPPKNKRDPEAKKPEDWDDNEYIPDPEDKK 238

Query: 121 PEGYDSXXXXXXXXXXXXXXXXXXXXXGVWKPRMIPNPEYKGRWKRKKIKNPNYKGKWKI 180
           PEGYD                      G W    IPNPEYKG W +KK+KNPNYKGKWK 
Sbjct: 239 PEGYDDIPKEITDPEATKPEDWDDEEDGEWTAPTIPNPEYKGPWNQKKLKNPNYKGKWKA 298

Query: 181 PWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVDEV 235
           P I NP+++DDP +Y    L ++GIE+WQVK+G++FDNILI DDPEYA+   +E 
Sbjct: 299 PLIPNPDYKDDPYIYAFDSLNHIGIELWQVKSGTLFDNILITDDPEYAKKFAEET 353



>Os01g0895600 Similar to Calreticulin 3 (Fragment)
          Length = 179

 Score =  133 bits (335), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 61/69 (88%), Positives = 65/69 (94%)

Query: 167 KKIKNPNYKGKWKIPWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPE 226
           +KIKNPNYKGKWKIPWIDNPEFEDDPDLYVLKPL+Y+GIEVWQVKAGSVFDNILICDDPE
Sbjct: 41  QKIKNPNYKGKWKIPWIDNPEFEDDPDLYVLKPLKYIGIEVWQVKAGSVFDNILICDDPE 100

Query: 227 YARSVVDEV 235
           YAR   +E 
Sbjct: 101 YARKAAEET 109



>Os04g0402100 Similar to Calnexin (Fragment)
          Length = 537

 Score =  125 bits (315), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 88/280 (31%), Positives = 131/280 (46%), Gaps = 57/280 (20%)

Query: 6   NQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQ------NYPIKKDLKCETDKLTHF 59
           + K+F  +TPY+ MFGPD CG  T K+H IL ++         + +K       DKL+H 
Sbjct: 120 DAKEFDNETPYTIMFGPDKCG-STNKVHFILKHKNPKTGKYVEHHLKFPPSVPYDKLSHV 178

Query: 60  YTFILRPDASYSLLVDNREREFGSMYTDWD----ILPPRKIKESNAKKPKDWDDREYIED 115
           YT IL+PD    +LVD  E++  +  +  D    ++P + I + + KKP+DWD+R  I D
Sbjct: 179 YTAILKPDNEVRILVDGEEKKKANFLSADDFEPSLIPSKTIPDPDDKKPEDWDERAKIPD 238

Query: 116 PDEVKPEGYDSXXXXXXXXXXXXXXXXXXXXXGVWKPRMIPNPEYK-------------- 161
           PD VKP+ +D                         +P  I +PE                
Sbjct: 239 PDAVKPDDWDEDAPMEIEDEEATKPEGWLDD----EPEEIDDPEANKPEDWDDEEDGEWE 294

Query: 162 ---------------GRWKRKKIKNPNYKGKWKIPWIDNPEFED--------DPDLYVL- 197
                          G WKR   +NP YKGKW  P IDNP ++         +P+ + L 
Sbjct: 295 APKIDNPKCEEAPGCGEWKRPMKQNPAYKGKWHAPLIDNPNYKGIWKPQEIPNPEYFELD 354

Query: 198 ----KPLQYVGIEVWQVKAGSVFDNILICDDPEYARSVVD 233
                P+  +GIE+W ++ G +FDNILI DD + A S+++
Sbjct: 355 KPDSDPIAAIGIEIWTMQDGILFDNILIADDEKVATSILE 394



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.141    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,971,337
Number of extensions: 371485
Number of successful extensions: 783
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 751
Number of HSP's successfully gapped: 5
Length of query: 302
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 202
Effective length of database: 11,814,401
Effective search space: 2386509002
Effective search space used: 2386509002
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 156 (64.7 bits)