BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0586200 Os04g0586200|AK120315
         (104 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0586200  Similar to Senescence-associated protein SAG102     161   1e-40
Os04g0586100  Protein of unknown function DUF581 family protein   122   5e-29
Os02g0687200  Protein of unknown function DUF581 family protein    76   4e-15
Os02g0686700  Protein of unknown function DUF581 family protein    66   6e-12
Os04g0585700  Protein of unknown function DUF581 family protein    65   1e-11

>Os04g0586200 Similar to Senescence-associated protein SAG102
          Length = 104

 Score =  161 bits (407), Expect = 1e-40,   Method: Compositional matrix adjust.
 Identities = 82/104 (78%), Positives = 82/104 (78%)

Query: 1   MAGSLACAFFFDAEPVGETGRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMH 60
           MAGSLACAFFFDAEPVGETGRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMH
Sbjct: 1   MAGSLACAFFFDAEPVGETGRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMH 60

Query: 61  LDXXXXXXXXXXXXXXXXXXXXXXHETAPAAPVSRKAGVSVASC 104
           LD                      HETAPAAPVSRKAGVSVASC
Sbjct: 61  LDAACARQAASARRKQQQQGQRSRHETAPAAPVSRKAGVSVASC 104



>Os04g0586100 Protein of unknown function DUF581 family protein
          Length = 97

 Score =  122 bits (307), Expect = 5e-29,   Method: Compositional matrix adjust.
 Identities = 56/62 (90%), Positives = 57/62 (91%)

Query: 1  MAGSLACAFFFDAEPVGETGRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMH 60
          MA SLACAFFFDAEPVGE GRHALDACALCTK L RDSDIFMY+GDTPFCSEECRYEQM 
Sbjct: 1  MAASLACAFFFDAEPVGEQGRHALDACALCTKQLTRDSDIFMYKGDTPFCSEECRYEQMQ 60

Query: 61 LD 62
          LD
Sbjct: 61 LD 62



>Os02g0687200 Protein of unknown function DUF581 family protein
          Length = 106

 Score = 76.3 bits (186), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 37/57 (64%), Positives = 43/57 (75%), Gaps = 4/57 (7%)

Query: 10 FFDAEPV--GET--GRHALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMHLD 62
          FFD EP+  GE     HA+DAC+LC KPL R+ DIFMYRG+TPFCSEECR  QM +D
Sbjct: 11 FFDIEPLDGGEACLSGHAMDACSLCRKPLTRNCDIFMYRGNTPFCSEECRDHQMEMD 67



>Os02g0686700 Protein of unknown function DUF581 family protein
          Length = 92

 Score = 65.9 bits (159), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 30/41 (73%), Positives = 31/41 (75%)

Query: 22 HALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMHLD 62
          H LDAC LC K L  + DIFMYRGDTPFCSEECR EQM  D
Sbjct: 8  HFLDACFLCRKRLAGNRDIFMYRGDTPFCSEECRREQMEAD 48



>Os04g0585700 Protein of unknown function DUF581 family protein
          Length = 150

 Score = 64.7 bits (156), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 27/41 (65%), Positives = 32/41 (78%)

Query: 22  HALDACALCTKPLRRDSDIFMYRGDTPFCSEECRYEQMHLD 62
           H L+AC LC KPL  + DI+MYRGD PFCSEECR EQ+ +D
Sbjct: 68  HFLEACFLCRKPLASNRDIYMYRGDIPFCSEECRREQIEMD 108



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.135    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,182,639
Number of extensions: 100217
Number of successful extensions: 219
Number of sequences better than 1.0e-10: 9
Number of HSP's gapped: 219
Number of HSP's successfully gapped: 9
Length of query: 104
Length of database: 17,035,801
Length adjustment: 72
Effective length of query: 32
Effective length of database: 13,276,393
Effective search space: 424844576
Effective search space used: 424844576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)