BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0257300 Os01g0257300|AK070626
         (109 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0257300  Conserved hypothetical protein                      185   5e-48
Os01g0933500  Conserved hypothetical protein                      127   2e-30
Os02g0460200  Conserved hypothetical protein                      120   2e-28
Os07g0671000  Conserved hypothetical protein                      111   9e-26
Os04g0282400  Similar to FPF1 protein-like (RAA1)                 111   9e-26
Os03g0346200  Conserved hypothetical protein                       89   5e-19

>Os01g0257300 Conserved hypothetical protein
          Length = 109

 Score =  185 bits (470), Expect = 5e-48,   Method: Compositional matrix adjust.
 Identities = 92/109 (84%), Positives = 92/109 (84%)

Query: 1   MSGVWVFKNGVVRLVEXXXXXXXXXXXXXXXXXLVHTPSGQVVSSYAALEARLTALGWER 60
           MSGVWVFKNGVVRLVE                 LVHTPSGQVVSSYAALEARLTALGWER
Sbjct: 1   MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVSSYAALEARLTALGWER 60

Query: 61  YYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVDA 109
           YYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVDA
Sbjct: 61  YYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVDA 109



>Os01g0933500 Conserved hypothetical protein
          Length = 127

 Score =  127 bits (319), Expect = 2e-30,   Method: Compositional matrix adjust.
 Identities = 67/126 (53%), Positives = 79/126 (62%), Gaps = 18/126 (14%)

Query: 1   MSGVWVFKNGVVRLVEXXXXXXXXXXXX----------XXXXXLVHTPSGQVVSSYAALE 50
           MSGVWVF+NGVV+LVE                           L+H P+G+VV+SYA+LE
Sbjct: 1   MSGVWVFRNGVVKLVENPPASANSGGGGGGGGGGGGGGIRRKALLHMPTGEVVTSYASLE 60

Query: 51  ARLTALGWERYYEDPS--------LFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD 102
            +L ALGWERYY            + QFHKR S+DLISLP DFS F SVHMYDIVVKNRD
Sbjct: 61  RKLAALGWERYYSGGGGAAAAAAMMLQFHKRSSVDLISLPKDFSQFGSVHMYDIVVKNRD 120

Query: 103 SFRVVD 108
           +FRV+D
Sbjct: 121 AFRVID 126



>Os02g0460200 Conserved hypothetical protein
          Length = 122

 Score =  120 bits (301), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 61/113 (53%), Positives = 71/113 (62%), Gaps = 4/113 (3%)

Query: 1   MSGVWVFKNGVVRLVE----XXXXXXXXXXXXXXXXXLVHTPSGQVVSSYAALEARLTAL 56
           M+GVWVFK+G+VR VE                     LVH PSG+VV+SY  LE RL  L
Sbjct: 1   MAGVWVFKDGIVRRVENPGSEESSSAGDGGGGGRRKVLVHVPSGEVVASYEVLERRLREL 60

Query: 57  GWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVDA 109
           GWERY  DP L QFH+R ++ LIS+P DFS F  VHMYDIVVK R+ F V DA
Sbjct: 61  GWERYLTDPCLLQFHQRSTVHLISVPRDFSKFKLVHMYDIVVKTRNVFEVRDA 113



>Os07g0671000 Conserved hypothetical protein
          Length = 105

 Score =  111 bits (278), Expect = 9e-26,   Method: Compositional matrix adjust.
 Identities = 59/108 (54%), Positives = 69/108 (63%), Gaps = 12/108 (11%)

Query: 3   GVWVFKN-GVVRLVEXXXXXXXXXXXXXXXXXLVHTPSGQVVSSYAALEARLTALGWERY 61
           GVWVF+N GV+ L E                 LVH  + +V+ S  ALE RL ALGWERY
Sbjct: 5   GVWVFRNNGVMELEEQATSRKA----------LVHVATSEVIRSTEALERRLGALGWERY 54

Query: 62  YEDPSLFQFHKR-GSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVD 108
           YED +  Q H+R GS DLIS+P DFS F S HMYD+VVKNRD F+VVD
Sbjct: 55  YEDRATLQLHRRDGSADLISIPRDFSRFRSTHMYDVVVKNRDHFKVVD 102



>Os04g0282400 Similar to FPF1 protein-like (RAA1)
          Length = 118

 Score =  111 bits (278), Expect = 9e-26,   Method: Compositional matrix adjust.
 Identities = 56/111 (50%), Positives = 68/111 (61%), Gaps = 2/111 (1%)

Query: 1   MSGVWVFKNGVVRLV--EXXXXXXXXXXXXXXXXXLVHTPSGQVVSSYAALEARLTALGW 58
           M+GVWVF++G+VR    E                 LVH PS +VV+SY  LE RL  LGW
Sbjct: 1   MAGVWVFEDGMVRRADSEAPSRGRGVGGGGGGGKVLVHVPSSEVVTSYEVLERRLRELGW 60

Query: 59  ERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRDSFRVVDA 109
           ERY  DP L QFH+R ++ LIS+P DFS    VHMYD+VVK R+ F V DA
Sbjct: 61  ERYLNDPCLLQFHQRSTVHLISVPRDFSRLKLVHMYDVVVKTRNVFEVRDA 111



>Os03g0346200 Conserved hypothetical protein
          Length = 68

 Score = 89.4 bits (220), Expect = 5e-19,   Method: Compositional matrix adjust.
 Identities = 41/67 (61%), Positives = 50/67 (74%), Gaps = 1/67 (1%)

Query: 43  VSSYAALEARLTALGWERYYEDPSLFQFHKR-GSLDLISLPADFSAFSSVHMYDIVVKNR 101
           + S  ALE RL  LGWERYYED ++ Q H+R G  DLISLP DF+ F S HMYD+V+KNR
Sbjct: 1   MGSLQALERRLAGLGWERYYEDRAVVQLHRRDGGADLISLPRDFARFRSTHMYDVVLKNR 60

Query: 102 DSFRVVD 108
           D F+V+D
Sbjct: 61  DHFKVLD 67



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,945,418
Number of extensions: 89446
Number of successful extensions: 139
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 131
Number of HSP's successfully gapped: 6
Length of query: 109
Length of database: 17,035,801
Length adjustment: 77
Effective length of query: 32
Effective length of database: 13,015,323
Effective search space: 416490336
Effective search space used: 416490336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 149 (62.0 bits)