BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0541900 Os12g0541900|AK069047
         (132 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0541900  Rapid ALkalinization Factor family protein          137   2e-33
Os12g0541700  Similar to Rapid alkalinization factor 2             68   2e-12
Os01g0257100  Rapid ALkalinization Factor family protein           65   9e-12
Os01g0357900  Similar to RALF                                      63   6e-11

>Os12g0541900 Rapid ALkalinization Factor family protein
          Length = 132

 Score =  137 bits (345), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 70/107 (65%), Positives = 70/107 (65%)

Query: 26  WSPSSLAGGASHHLRLEEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIDKN 85
           WSPSSLAGGASHHLRLEEV                                     IDKN
Sbjct: 26  WSPSSLAGGASHHLRLEEVASAAGAGAAGHGSMSAAAGKDGGASAAAHGSAPAAAGIDKN 85

Query: 86  GYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG 132
           GYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG
Sbjct: 86  GYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG 132



>Os12g0541700 Similar to Rapid alkalinization factor 2
          Length = 127

 Score = 67.8 bits (164), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 30/47 (63%), Positives = 38/47 (80%), Gaps = 1/47 (2%)

Query: 86  GYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG 132
           GY+S+  ++RRDSVPC+R+GASYYNC PGA  +PY+R C  ITRC G
Sbjct: 82  GYISY-QSLRRDSVPCSRRGASYYNCRPGASANPYHRGCSRITRCRG 127



>Os01g0257100 Rapid ALkalinization Factor family protein
          Length = 131

 Score = 65.5 bits (158), Expect = 9e-12,   Method: Compositional matrix adjust.
 Identities = 28/48 (58%), Positives = 38/48 (79%), Gaps = 1/48 (2%)

Query: 85  NGYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG 132
           +GY+ +  A+RRDSVPC+++GASYYNC PGA  +PY+R C  IT+C G
Sbjct: 85  SGYIGY-DALRRDSVPCSQRGASYYNCQPGAEANPYSRGCSAITQCRG 131



>Os01g0357900 Similar to RALF
          Length = 117

 Score = 62.8 bits (151), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 27/51 (52%), Positives = 36/51 (70%), Gaps = 1/51 (1%)

Query: 82  IDKNGYLSFFAAMRRDSVPCTRKGASYYNCVPGAPPSPYNRSCEHITRCHG 132
           +   GY+S+  A+RRD+ PC+ +GASYYNC PG   +PY+R C  ITRC G
Sbjct: 68  LQGQGYISY-GALRRDTTPCSVRGASYYNCRPGGQANPYSRGCSAITRCRG 117



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,107,189
Number of extensions: 73181
Number of successful extensions: 158
Number of sequences better than 1.0e-10: 7
Number of HSP's gapped: 157
Number of HSP's successfully gapped: 7
Length of query: 132
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 42
Effective length of database: 12,336,541
Effective search space: 518134722
Effective search space used: 518134722
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 150 (62.4 bits)