BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0290100 Os12g0290100|016-083-B05
         (170 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0290100  Protein kinase-like domain containing protein       348   8e-97
Os10g0476100  Protein kinase-like domain containing protein        95   2e-20
Os05g0511400  Similar to Protein kinase GhCLK1 (Fragment)          92   1e-19
Os01g0186700  Similar to Protein kinase GhCLK1 (Fragment)          75   3e-14

>Os12g0290100 Protein kinase-like domain containing protein
          Length = 170

 Score =  348 bits (894), Expect = 8e-97,   Method: Compositional matrix adjust.
 Identities = 170/170 (100%), Positives = 170/170 (100%)

Query: 1   RYYAKLYYRRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTII 60
           RYYAKLYYRRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTII
Sbjct: 1   RYYAKLYYRRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTII 60

Query: 61  GRGAFGEVRRALFLLPIFDADIVDCCVMRNKHQFLQYLEKPFIIHGEKKSIFRGNIYVLC 120
           GRGAFGEVRRALFLLPIFDADIVDCCVMRNKHQFLQYLEKPFIIHGEKKSIFRGNIYVLC
Sbjct: 61  GRGAFGEVRRALFLLPIFDADIVDCCVMRNKHQFLQYLEKPFIIHGEKKSIFRGNIYVLC 120

Query: 121 FSCSLPPVYIFILQVRLCREKATSNVYAMKKLKKSEMLRRGQVIHVCLSN 170
           FSCSLPPVYIFILQVRLCREKATSNVYAMKKLKKSEMLRRGQVIHVCLSN
Sbjct: 121 FSCSLPPVYIFILQVRLCREKATSNVYAMKKLKKSEMLRRGQVIHVCLSN 170



>Os10g0476100 Protein kinase-like domain containing protein
          Length = 561

 Score = 95.1 bits (235), Expect = 2e-20,   Method: Composition-based stats.
 Identities = 64/158 (40%), Positives = 76/158 (48%), Gaps = 67/158 (42%)

Query: 9   RRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTIIGRGAFGEV 68
           RR  LE KLAD +VSEEEQ+NI+K FE +E E MR +RHKM VDDF+LLT+IG+GAFGEV
Sbjct: 80  RRCSLEKKLADANVSEEEQHNIVKQFEKKETEYMRMQRHKMSVDDFDLLTMIGKGAFGEV 139

Query: 69  RRALFLLPIFDADIVDCCVMRNKHQFLQYLEKPFIIHGEKKSIFRGNIYVLCFSCSLPPV 128
           R                 V R K+                     GN+Y +         
Sbjct: 140 R-----------------VCREKNT--------------------GNVYAM--------- 153

Query: 129 YIFILQVRLCREKATSNVYAMKKLKKSEMLRRGQVIHV 166
                                KKL+KSEMLRRGQV HV
Sbjct: 154 ---------------------KKLRKSEMLRRGQVEHV 170



>Os05g0511400 Similar to Protein kinase GhCLK1 (Fragment)
          Length = 556

 Score = 92.4 bits (228), Expect = 1e-19,   Method: Composition-based stats.
 Identities = 45/61 (73%), Positives = 49/61 (80%)

Query: 9   RRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTIIGRGAFGEV 68
           RR MLE KL D +V  EEQNNILK  E +E E MR +RHKMGV+DFELLTIIGRGAFGEV
Sbjct: 71  RRWMLERKLQDAEVPVEEQNNILKHLEKKETEYMRLQRHKMGVEDFELLTIIGRGAFGEV 130

Query: 69  R 69
           R
Sbjct: 131 R 131



>Os01g0186700 Similar to Protein kinase GhCLK1 (Fragment)
          Length = 544

 Score = 75.1 bits (183), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 39/61 (63%), Positives = 45/61 (73%)

Query: 9   RRRMLESKLADPDVSEEEQNNILKDFENREREIMRSRRHKMGVDDFELLTIIGRGAFGEV 68
           RR  LE +L    V  E+Q N+LKD E +E E MR +RHK+ VDDFELLTIIGRGAFGEV
Sbjct: 71  RRFRLERQLESSQVPREQQINLLKDLERKETEYMRLKRHKICVDDFELLTIIGRGAFGEV 130

Query: 69  R 69
           R
Sbjct: 131 R 131



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.329    0.143    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,546,501
Number of extensions: 211186
Number of successful extensions: 938
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 938
Number of HSP's successfully gapped: 4
Length of query: 170
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 76
Effective length of database: 12,127,685
Effective search space: 921704060
Effective search space used: 921704060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 152 (63.2 bits)