BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0245100 Os12g0245100|AK065859
         (477 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0245100  Hypothetical protein                                942   0.0  
Os12g0245000  Hypothetical protein                                190   2e-48
Os04g0127800  Conserved hypothetical protein                      148   9e-36
Os08g0564800  Conserved hypothetical protein                       86   6e-17

>Os12g0245100 Hypothetical protein
          Length = 477

 Score =  942 bits (2435), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 452/477 (94%), Positives = 452/477 (94%)

Query: 1   MAAAGGGSLLDQSRHAMRSWVLKNIEKLAGHYGAEFNSLRAFEDRVFNETSSIDDYISKI 60
           MAAAGGGSLLDQSRHAMRSWVLKNIEKLAGHYGAEFNSLRAFEDRVFNETSSIDDYISKI
Sbjct: 1   MAAAGGGSLLDQSRHAMRSWVLKNIEKLAGHYGAEFNSLRAFEDRVFNETSSIDDYISKI 60

Query: 61  STKMAILERPXXXXXXXXXXXXXXXTRLPTTVMXXXXXXXXXXHLMSNNHHQTHGGWQSN 120
           STKMAILERP               TRLPTTVM          HLMSNNHHQTHGGWQSN
Sbjct: 61  STKMAILERPQSSSGMQHQGQMGNQTRLPTTVMQEQPQPQQQPHLMSNNHHQTHGGWQSN 120

Query: 121 VARVQMITGVGEVNWREEMFQKISVLKNSFFSELTDFDRLLHNCQKTEEQLQSLPKKQAD 180
           VARVQMITGVGEVNWREEMFQKISVLKNSFFSELTDFDRLLHNCQKTEEQLQSLPKKQAD
Sbjct: 121 VARVQMITGVGEVNWREEMFQKISVLKNSFFSELTDFDRLLHNCQKTEEQLQSLPKKQAD 180

Query: 181 QYKRITKLKDAVRSALDLLQLQKSSIDEGMKVKFCKYESSIHSLLRFYRETKAKINEMNA 240
           QYKRITKLKDAVRSALDLLQLQKSSIDEGMKVKFCKYESSIHSLLRFYRETKAKINEMNA
Sbjct: 181 QYKRITKLKDAVRSALDLLQLQKSSIDEGMKVKFCKYESSIHSLLRFYRETKAKINEMNA 240

Query: 241 NRHNNQQEQPAGLPRQRITDRTPSSARRQNRNDNVIGQSEDKLRCRVESVVAKKKPIDRL 300
           NRHNNQQEQPAGLPRQRITDRTPSSARRQNRNDNVIGQSEDKLRCRVESVVAKKKPIDRL
Sbjct: 241 NRHNNQQEQPAGLPRQRITDRTPSSARRQNRNDNVIGQSEDKLRCRVESVVAKKKPIDRL 300

Query: 301 INALRHSVEDDRTDVKRQKTRHVNSALANEIDAMNAKLIDTVVRIAGEKDGGTEIEFSYT 360
           INALRHSVEDDRTDVKRQKTRHVNSALANEIDAMNAKLIDTVVRIAGEKDGGTEIEFSYT
Sbjct: 301 INALRHSVEDDRTDVKRQKTRHVNSALANEIDAMNAKLIDTVVRIAGEKDGGTEIEFSYT 360

Query: 361 AVSLALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDGDEQRRGMFGEISGMV 420
           AVSLALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDGDEQRRGMFGEISGMV
Sbjct: 361 AVSLALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDGDEQRRGMFGEISGMV 420

Query: 421 SAAFHCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFSSRHGQWMDCTVE 477
           SAAFHCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFSSRHGQWMDCTVE
Sbjct: 421 SAAFHCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFSSRHGQWMDCTVE 477



>Os12g0245000 Hypothetical protein
          Length = 318

 Score =  190 bits (483), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 114/252 (45%), Positives = 145/252 (57%), Gaps = 19/252 (7%)

Query: 226 RFYRETKAKINEMNANRHNNQQEQPAGLPRQRITDRTPSSARRQNRNDNVIGQSEDKLRC 285
           R  RE   KI E NA+R + +  +P G P          + R++   +  I      +  
Sbjct: 82  RNIREMNGKIKETNASRESPRILKPTGEPNPSTGGIRIQNNRQEQPAEMTIFHPWKNITD 141

Query: 286 RVESVVAKKKPIDRLINALRHSVEDDRTDVKRQKTRHVNSALANEIDAMNAKLIDTVVRI 345
           R  +       ID L+N      EDD T  KRQK + V+  L  EI+ +N +L DTV+ I
Sbjct: 142 RTSAYELSH--IDNLVNH-----EDDET--KRQKEQSVHITLLYEINLINEELFDTVISI 192

Query: 346 AGEKDGGTEIEFSYTAVSLALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDG 405
            G K GGT I+FSY AVSLA DM+  FAAYGTSP+KP KLFVPADYPRSSPV     +DG
Sbjct: 193 TGHKHGGTVIKFSYNAVSLAQDMELPFAAYGTSPLKPAKLFVPADYPRSSPVY----EDG 248

Query: 406 DEQRRGMFGEISGMVSAAFHCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFS 465
           DEQ +GM+  ISGMV   F  ALR+LP  MS++ MA +W   V+ +       +GGGTFS
Sbjct: 249 DEQHQGMYSVISGMVDKVFQRALRKLPVPMSIRDMARQWGISVRTVT------NGGGTFS 302

Query: 466 SRHGQWMDCTVE 477
           S +GQW  CT E
Sbjct: 303 SGYGQWESCTDE 314



>Os04g0127800 Conserved hypothetical protein
          Length = 988

 Score =  148 bits (373), Expect = 9e-36,   Method: Compositional matrix adjust.
 Identities = 80/168 (47%), Positives = 107/168 (63%), Gaps = 15/168 (8%)

Query: 314 DVKRQKTR-HVNSALANEIDAMNAKLIDTVVRIAGEKD---------GGTEIEFSYTAVS 363
           + KRQKT+ + N AL +EI  +N KL+DTV+  A E           GG  I+ SY ++S
Sbjct: 814 NAKRQKTQVNANDALVDEIKTINNKLVDTVMNFADENGTDEIIYQNGGGMLIKLSYISMS 873

Query: 364 LALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDGDEQRRGMFGEISGMVSAA 423
           L+  +K LFAA   + V PVKL VPADYP+SSP++ +N+D   EQRR    +IS  V+ A
Sbjct: 874 LSPSLKSLFAASEMTIVMPVKLLVPADYPKSSPILVDNDD---EQRR--LSDISYAVAVA 928

Query: 424 FHCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFSSRHGQW 471
           F CA+ ELP   S++ MA  W+ CV+  + + A RHGGGTFSSRH QW
Sbjct: 929 FGCAVDELPEPRSIEAMAMAWDGCVRRAVTEVAHRHGGGTFSSRHNQW 976




 Score = 87.0 bits (214), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 42/111 (37%), Positives = 73/111 (65%)

Query: 124 VQMITGVGEVNWREEMFQKISVLKNSFFSELTDFDRLLHNCQKTEEQLQSLPKKQADQYK 183
           ++ I   GEV+WREE+FQKI   K+++ SE+ ++D+++H  + TEEQL+SLP + A++ +
Sbjct: 318 LEPIVIAGEVDWREEIFQKIKSFKDAYLSEVLEYDQIVHVPKLTEEQLRSLPVENAEKLR 377

Query: 184 RITKLKDAVRSALDLLQLQKSSIDEGMKVKFCKYESSIHSLLRFYRETKAK 234
           RI  +K  +   LDLL  QKS++ +GM+  F  ++  +  L     ++KA+
Sbjct: 378 RIRHVKKIIAIMLDLLNTQKSNVRKGMQNIFPIFQQYLGQLRLSISKSKAR 428



>Os08g0564800 Conserved hypothetical protein
          Length = 1320

 Score = 85.9 bits (211), Expect = 6e-17,   Method: Compositional matrix adjust.
 Identities = 60/170 (35%), Positives = 99/170 (58%), Gaps = 15/170 (8%)

Query: 315  VKRQKTRHVNSALANEIDAMNAKLIDTVVRI----------AGEKDGGTEIEFSYTAVSL 364
            VK QKT  +N AL  EI  +N +LIDT + +            E   GT I+ ++TAV++
Sbjct: 1153 VKWQKTE-INHALMEEIHEINQQLIDTELHVCEDDAESFGATSEGTEGTVIKCTFTAVAV 1211

Query: 365  ALDMKQLFAAYGTSPVKPVKLFVPADYPRSSPVVSNNNDDGDEQRRGMFGEISGMVSAAF 424
            +  +K +FA+   SP+ P+++ VPA YP+ SPV+ +     DEQR     ++S    + F
Sbjct: 1212 SPSLKSMFASAQMSPILPLRVLVPASYPKCSPVLLDKFP--DEQRNS--DDLSTKARSKF 1267

Query: 425  HCALRELPPSMSVKQMASEWNSCVQMIMKKFAIRHGGGTFSSRHGQWMDC 474
               LR L   MS++++A  W++C + ++ ++A + GGG+FSS +G W +C
Sbjct: 1268 SILLRGLAEPMSLREIARTWDACARKVIAEYAQQTGGGSFSSSYGCWENC 1317



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.130    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,035,288
Number of extensions: 571050
Number of successful extensions: 1811
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1805
Number of HSP's successfully gapped: 5
Length of query: 477
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 372
Effective length of database: 11,553,331
Effective search space: 4297839132
Effective search space used: 4297839132
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)