BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0163800 Os12g0163800|J100060A02
(61 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0163800 Protein kinase domain containing protein 125 5e-30
Os07g0203900 Protein prenyltransferase domain containing pr... 119 5e-28
Os09g0544300 Amino acid-binding ACT domain containing protein 82 1e-16
Os02g0120100 Amino acid-binding ACT domain containing protein 79 6e-16
Os06g0724900 Amino acid-binding ACT domain containing protein 78 1e-15
Os04g0686650 Protein kinase domain containing protein 77 3e-15
Os07g0475900 Amino acid-binding ACT domain containing protein 70 4e-13
>Os12g0163800 Protein kinase domain containing protein
Length = 61
Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust.
Identities = 61/61 (100%), Positives = 61/61 (100%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHVRS 60
MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHVRS
Sbjct: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHVRS 60
Query: 61 L 61
L
Sbjct: 61 L 61
>Os07g0203900 Protein prenyltransferase domain containing protein
Length = 721
Score = 119 bits (298), Expect = 5e-28, Method: Compositional matrix adjust.
Identities = 55/58 (94%), Positives = 57/58 (98%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHV 58
MSGGSLYDF+HKQHNVLDLPTLLKFAVD+CRGMCYLHQ GIIHRDLKSANLLMDKDHV
Sbjct: 551 MSGGSLYDFLHKQHNVLDLPTLLKFAVDLCRGMCYLHQMGIIHRDLKSANLLMDKDHV 608
>Os09g0544300 Amino acid-binding ACT domain containing protein
Length = 603
Score = 81.6 bits (200), Expect = 1e-16, Method: Composition-based stats.
Identities = 35/56 (62%), Positives = 43/56 (76%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKD 56
MSGGS+YD++HK V LP LL +DV +GM YLHQ IIHRDLK+ANLLMD++
Sbjct: 399 MSGGSVYDYLHKHKGVFKLPALLGVVMDVSKGMSYLHQNNIIHRDLKTANLLMDEN 454
>Os02g0120100 Amino acid-binding ACT domain containing protein
Length = 583
Score = 79.0 bits (193), Expect = 6e-16, Method: Composition-based stats.
Identities = 34/58 (58%), Positives = 46/58 (79%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHV 58
MSGGSL+D++HK++N L +L+ A D+ +GM YLHQ IIHRDLK+ANLLMD++ V
Sbjct: 382 MSGGSLHDYLHKKNNSFKLSEILRVATDISKGMNYLHQNNIIHRDLKTANLLMDENKV 439
>Os06g0724900 Amino acid-binding ACT domain containing protein
Length = 564
Score = 78.2 bits (191), Expect = 1e-15, Method: Composition-based stats.
Identities = 34/55 (61%), Positives = 43/55 (78%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDK 55
M GS+YD++HK+ LP+LLK AVD+ +GM YLHQ IIHRDLK+ANLLMD+
Sbjct: 362 MKNGSVYDYLHKRKGSFKLPSLLKAAVDISKGMNYLHQNKIIHRDLKTANLLMDE 416
>Os04g0686650 Protein kinase domain containing protein
Length = 138
Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 34/58 (58%), Positives = 45/58 (77%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHV 58
M GG+LYDF+HKQ+N L+LP +L+ A+ + +GM YLHQ IIHRDLK+ANLL+ V
Sbjct: 58 MPGGNLYDFLHKQNNTLELPVVLRIAIGISKGMDYLHQNNIIHRDLKTANLLIGSGQV 115
>Os07g0475900 Amino acid-binding ACT domain containing protein
Length = 438
Score = 70.1 bits (170), Expect = 4e-13, Method: Composition-based stats.
Identities = 31/59 (52%), Positives = 41/59 (69%)
Query: 1 MSGGSLYDFVHKQHNVLDLPTLLKFAVDVCRGMCYLHQRGIIHRDLKSANLLMDKDHVR 59
M GGS++DF++ LP +L+ A DV +GM YLHQ I+HRDLK+ANLLMD V+
Sbjct: 239 MRGGSIFDFLYNFRGTFQLPDVLRIASDVSKGMNYLHQINIVHRDLKTANLLMDDQVVK 297
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.329 0.143 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,178,857
Number of extensions: 65381
Number of successful extensions: 1834
Number of sequences better than 1.0e-10: 7
Number of HSP's gapped: 1864
Number of HSP's successfully gapped: 7
Length of query: 61
Length of database: 17,035,801
Length adjustment: 33
Effective length of query: 28
Effective length of database: 15,312,739
Effective search space: 428756692
Effective search space used: 428756692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)