BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0144600 Os12g0144600|J090083M18
(524 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0144600 Heavy metal transport/detoxification protein d... 182 7e-46
Os11g0147500 Heavy metal transport/detoxification protein d... 181 9e-46
Os01g0125600 Heavy metal transport/detoxification protein d... 100 2e-21
Os05g0150700 Heavy metal transport/detoxification protein d... 88 1e-17
Os05g0368600 Heavy metal transport/detoxification protein d... 74 4e-13
Os01g0933200 Heavy metal transport/detoxification protein d... 70 3e-12
AK063326 70 3e-12
Os03g0120400 Heavy metal transport/detoxification protein d... 66 5e-11
>Os12g0144600 Heavy metal transport/detoxification protein domain containing
protein
Length = 524
Score = 182 bits (461), Expect = 7e-46, Method: Compositional matrix adjust.
Identities = 87/87 (100%), Positives = 87/87 (100%)
Query: 1 MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI 60
MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI
Sbjct: 1 MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI 60
Query: 61 IKKLNKAGKPAELWGSKVGVAAVNNQF 87
IKKLNKAGKPAELWGSKVGVAAVNNQF
Sbjct: 61 IKKLNKAGKPAELWGSKVGVAAVNNQF 87
>Os11g0147500 Heavy metal transport/detoxification protein domain containing
protein
Length = 515
Score = 181 bits (460), Expect = 9e-46, Method: Compositional matrix adjust.
Identities = 87/87 (100%), Positives = 87/87 (100%)
Query: 1 MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI 60
MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI
Sbjct: 1 MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI 60
Query: 61 IKKLNKAGKPAELWGSKVGVAAVNNQF 87
IKKLNKAGKPAELWGSKVGVAAVNNQF
Sbjct: 61 IKKLNKAGKPAELWGSKVGVAAVNNQF 87
>Os01g0125600 Heavy metal transport/detoxification protein domain containing
protein
Length = 420
Score = 100 bits (250), Expect = 2e-21, Method: Compositional matrix adjust.
Identities = 51/93 (54%), Positives = 65/93 (69%), Gaps = 8/93 (8%)
Query: 1 MSKEE---VLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDP 57
M+K+E ++KIQT VL+VNIHCDGC+ KVKK+L KIEGVY ++D + KVTV+G VD
Sbjct: 1 MTKDEDFKLVKIQTHVLRVNIHCDGCKHKVKKLLQKIEGVYSVALDVDNHKVTVTGNVDS 60
Query: 58 ATIIKKLNKAGKPAELW-----GSKVGVAAVNN 85
T+I+KL + GK AELW GS G NN
Sbjct: 61 DTLIRKLTRGGKHAELWSQQKGGSNQGHKGSNN 93
>Os05g0150700 Heavy metal transport/detoxification protein domain containing
protein
Length = 368
Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 43/70 (61%), Positives = 51/70 (72%)
Query: 5 EVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATIIKKL 64
E LK QT LKV+IHC+GC+KKVKK+LH IEGVY+T ID + KV V G V T++KKL
Sbjct: 9 EPLKYQTLALKVSIHCEGCKKKVKKVLHSIEGVYKTDIDVQHQKVVVIGNVSVDTLVKKL 68
Query: 65 NKAGKPAELW 74
K GK AE W
Sbjct: 69 VKTGKHAEPW 78
>Os05g0368600 Heavy metal transport/detoxification protein domain containing
protein
Length = 340
Score = 73.6 bits (179), Expect = 4e-13, Method: Compositional matrix adjust.
Identities = 33/69 (47%), Positives = 49/69 (71%), Gaps = 2/69 (2%)
Query: 5 EVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGK--VTVSGLVDPATIIK 62
E L+ + L+V+IHC+GC+KKVKK+L ++EGVY+ +D K VTV+G V T+++
Sbjct: 4 ESLQCKVLALRVSIHCEGCKKKVKKVLQRVEGVYRCDVDGRSNKATVTVTGKVSADTLVR 63
Query: 63 KLNKAGKPA 71
KL +AGK A
Sbjct: 64 KLRRAGKHA 72
>Os01g0933200 Heavy metal transport/detoxification protein domain containing
protein
Length = 336
Score = 70.5 bits (171), Expect = 3e-12, Method: Compositional matrix adjust.
Identities = 34/75 (45%), Positives = 49/75 (65%), Gaps = 2/75 (2%)
Query: 2 SKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGK--VTVSGLVDPAT 59
S E ++ Q VL+V+IHC+GC+KKVKK+L + GV++ +DA K VT S +D
Sbjct: 3 SGSEPIECQVLVLRVSIHCEGCKKKVKKVLQHVPGVFRCDVDARSNKVIVTASRNMDANI 62
Query: 60 IIKKLNKAGKPAELW 74
++ KL K+GK AE W
Sbjct: 63 LVAKLRKSGKQAEPW 77
>AK063326
Length = 336
Score = 70.5 bits (171), Expect = 3e-12, Method: Compositional matrix adjust.
Identities = 34/75 (45%), Positives = 49/75 (65%), Gaps = 2/75 (2%)
Query: 2 SKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGK--VTVSGLVDPAT 59
S E ++ Q VL+V+IHC+GC+KKVKK+L + GV++ +DA K VT S +D
Sbjct: 3 SGSEPIECQVLVLRVSIHCEGCKKKVKKVLQHVPGVFRCDVDARSNKVIVTASRNMDANI 62
Query: 60 IIKKLNKAGKPAELW 74
++ KL K+GK AE W
Sbjct: 63 LVAKLRKSGKQAEPW 77
>Os03g0120400 Heavy metal transport/detoxification protein domain containing
protein
Length = 193
Score = 66.2 bits (160), Expect = 5e-11, Method: Compositional matrix adjust.
Identities = 30/74 (40%), Positives = 45/74 (60%)
Query: 1 MSKEEVLKIQTCVLKVNIHCDGCQKKVKKILHKIEGVYQTSIDAEQGKVTVSGLVDPATI 60
M + L +QT LKV + C GC++ VK L K+ GV ++ E KVTV+G V+ +
Sbjct: 54 MGRSRPLSLQTVELKVRMCCSGCERVVKHALMKLRGVDSVEVELEMEKVTVTGYVERQRV 113
Query: 61 IKKLNKAGKPAELW 74
+K++ +AGK AE W
Sbjct: 114 LKEVRRAGKKAEFW 127
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.314 0.131 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,610,421
Number of extensions: 102936
Number of successful extensions: 324
Number of sequences better than 1.0e-10: 9
Number of HSP's gapped: 323
Number of HSP's successfully gapped: 9
Length of query: 524
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 419
Effective length of database: 11,553,331
Effective search space: 4840845689
Effective search space used: 4840845689
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 158 (65.5 bits)