BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0125400 Os12g0125400|AK062006
(384 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0125400 Similar to Homoserine dehydrogenase-like protein 677 0.0
Os11g0128800 Similar to Homoserine dehydrogenase-like protein 660 0.0
Os08g0342400 Similar to Bifunctional aspartokinase/homoseri... 167 1e-41
Os09g0294000 Similar to Bifunctional aspartokinase/homoseri... 159 5e-39
Os05g0295700 Similar to Homoserine dehydrogenase-like protein 111 8e-25
>Os12g0125400 Similar to Homoserine dehydrogenase-like protein
Length = 384
Score = 677 bits (1748), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 344/384 (89%), Positives = 344/384 (89%)
Query: 1 MAAPVRSXXXXXXXXXXXXXXXXXRHILSCRPLHANQGVAIRVLGVADSSSLLVADDLHS 60
MAAPVRS RHILSCRPLHANQGVAIRVLGVADSSSLLVADDLHS
Sbjct: 1 MAAPVRSVLPVVLLGCGGVGRHLLRHILSCRPLHANQGVAIRVLGVADSSSLLVADDLHS 60
Query: 61 NGFXXXXXXXXXXXKSAGSPLSSLLSRGQCQLFNNPEARRKVIDTASVLGKTTGLVLVDC 120
NGF KSAGSPLSSLLSRGQCQLFNNPEARRKVIDTASVLGKTTGLVLVDC
Sbjct: 61 NGFDDALLADLCAAKSAGSPLSSLLSRGQCQLFNNPEARRKVIDTASVLGKTTGLVLVDC 120
Query: 121 SATYDTVGMLKDAVDRGCCVVLANKKPLTCAYEDFEKLVSNFRRMRFESTVGAGLPVIAS 180
SATYDTVGMLKDAVDRGCCVVLANKKPLTCAYEDFEKLVSNFRRMRFESTVGAGLPVIAS
Sbjct: 121 SATYDTVGMLKDAVDRGCCVVLANKKPLTCAYEDFEKLVSNFRRMRFESTVGAGLPVIAS 180
Query: 181 VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSLGYTEPDPRDDLSGMD 240
VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSLGYTEPDPRDDLSGMD
Sbjct: 181 VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSLGYTEPDPRDDLSGMD 240
Query: 241 VARKALILARLLGQRISMENINVESLYPSEFGPDAMSTKDFLESGLVQLDKSIEERVKAA 300
VARKALILARLLGQRISMENINVESLYPSEFGPDAMSTKDFLESGLVQLDKSIEERVKAA
Sbjct: 241 VARKALILARLLGQRISMENINVESLYPSEFGPDAMSTKDFLESGLVQLDKSIEERVKAA 300
Query: 301 SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQXXXX 360
SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQ
Sbjct: 301 SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQGAGA 360
Query: 361 XXXXXXXXVLADILDLQDLFHKTA 384
VLADILDLQDLFHKTA
Sbjct: 361 GNDTTAAGVLADILDLQDLFHKTA 384
>Os11g0128800 Similar to Homoserine dehydrogenase-like protein
Length = 384
Score = 660 bits (1702), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 335/384 (87%), Positives = 340/384 (88%)
Query: 1 MAAPVRSXXXXXXXXXXXXXXXXXRHILSCRPLHANQGVAIRVLGVADSSSLLVADDLHS 60
MAAPVRS RHI+SCRPLHANQGVAIRVLGVADSSSLLVADDLHS
Sbjct: 1 MAAPVRSVLPVVLLGCGGVGRHLLRHIVSCRPLHANQGVAIRVLGVADSSSLLVADDLHS 60
Query: 61 NGFXXXXXXXXXXXKSAGSPLSSLLSRGQCQLFNNPEARRKVIDTASVLGKTTGLVLVDC 120
NGF KSAGSPLSSLL+RGQCQLFNN EARRKVIDTASVLGKTTGLVLVDC
Sbjct: 61 NGFDDALLADLCAAKSAGSPLSSLLARGQCQLFNNTEARRKVIDTASVLGKTTGLVLVDC 120
Query: 121 SATYDTVGMLKDAVDRGCCVVLANKKPLTCAYEDFEKLVSNFRRMRFESTVGAGLPVIAS 180
SATYDTVGMLKDAVD GCCVVLANKKPLTCAYEDFEKLVSNFRR+RFESTVGA LPVIAS
Sbjct: 121 SATYDTVGMLKDAVDCGCCVVLANKKPLTCAYEDFEKLVSNFRRIRFESTVGASLPVIAS 180
Query: 181 VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSLGYTEPDPRDDLSGMD 240
VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGK+FSEVVKTAKSLGYTEPDPRDDLSGMD
Sbjct: 181 VTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKKFSEVVKTAKSLGYTEPDPRDDLSGMD 240
Query: 241 VARKALILARLLGQRISMENINVESLYPSEFGPDAMSTKDFLESGLVQLDKSIEERVKAA 300
VARKALILARLLGQ+ISMENINVESLYPSE GPDAMSTKDFLESGLVQLDKSIEERVKAA
Sbjct: 241 VARKALILARLLGQQISMENINVESLYPSELGPDAMSTKDFLESGLVQLDKSIEERVKAA 300
Query: 301 SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQXXXX 360
SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQ
Sbjct: 301 SLKGNVLRYVCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYESAPLVIQGAGA 360
Query: 361 XXXXXXXXVLADILDLQDLFHKTA 384
VLADILDLQDLFHKTA
Sbjct: 361 GNDTTAAGVLADILDLQDLFHKTA 384
>Os08g0342400 Similar to Bifunctional aspartokinase/homoserine dehydrogenase 1,
chloroplast precursor (AK-HD 1) (AK-HSDH 1) [Includes:
Aspartokinase (EC 2.7.2.4)
Length = 763
Score = 167 bits (423), Expect = 1e-41, Method: Compositional matrix adjust.
Identities = 113/350 (32%), Positives = 169/350 (48%), Gaps = 30/350 (8%)
Query: 33 LHANQGVAIRVLGVADSSSLLVADDLHSNGFXXXXXXXXXXXKSAGSPLSSLLSRGQCQL 92
L N + +RV+G+ S +++++D G L+ + Q Q
Sbjct: 430 LKENMNIDLRVMGITGSRTMVLSD--------------------TGIDLAHW--KEQLQT 467
Query: 93 FNNPEARRKVIDTASVLGKTTGLVLVDCSATYDTVGMLKDAVDRGCCVVLANKKPLTCAY 152
P K +D S VLVDC+A D + +G V+ NKK +
Sbjct: 468 EAEPANLDKFVDHLSENQLFPNRVLVDCTADTSVASHYYDWLKKGIHVITPNKKANSGPL 527
Query: 153 EDFEKLVS----NFRRMRFESTVGAGLPVIASVTRIIASGDPVSRIVGSLSGTLGYVMSE 208
+ + KL + ++ +E+TVGAGLP+I+++ ++ +GD + RI G SGTL Y+ +
Sbjct: 528 DKYLKLRTLQRASYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGIFSGTLSYIFNN 587
Query: 209 LEDGKRFSEVVKTAKSLGYTEPDPRDDLSGMDVARKALILARLLGQRISMENINVESLYP 268
E + FS+VV AK GYTEPDPRDDLSG DVARK +ILAR G ++ + +I V SL P
Sbjct: 588 FEGTRAFSDVVSEAKEAGYTEPDPRDDLSGTDVARKVIILARESGLKLELSDIPVRSLVP 647
Query: 269 SEFGPDAMSTKDFLESGLVQLDKSIEERVKAASLKGNVLRYVCKIE--STGCQVGLEELP 326
+ ST D L D+ K A G VLRYV ++ + QV L
Sbjct: 648 EAL--RSCSTADEYMQKLPSFDQDWARESKDAEAAGEVLRYVGVVDLVNKEGQVELRRYK 705
Query: 327 KNSALGRLRGSDNVVEIYSRCYESAPLVIQXXXXXXXXXXXXVLADILDL 376
K+ +L GSDN++ + Y+ PL+++ V +DIL L
Sbjct: 706 KDHPFAQLSGSDNIIAFTTSRYKEQPLIVRGPGAGAEVTAGGVFSDILRL 755
>Os09g0294000 Similar to Bifunctional aspartokinase/homoserine dehydrogenase 2,
chloroplast precursor (AK-HD 2) (AK-HSDH 2) [Includes:
Aspartokinase (EC 2.7.2.4)
Length = 915
Score = 159 bits (401), Expect = 5e-39, Method: Compositional matrix adjust.
Identities = 95/267 (35%), Positives = 142/267 (53%), Gaps = 8/267 (2%)
Query: 116 VLVDCSATYDTVGMLKDAVDRGCCVVLANKKPLTCAYEDFEKLVS----NFRRMRFESTV 171
VLVDC+A D + +G V+ NKK + + + KL + ++ +E+TV
Sbjct: 643 VLVDCTADTYVACHYYDWLKKGIHVITPNKKANSGPLDRYLKLRTLQRASYTHYFYEATV 702
Query: 172 GAGLPVIASVTRIIASGDPVSRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSLGYTEPD 231
GAGLP+I+++ ++ +GD + RI G SGTL Y+ + E + FS VV AK GYTEPD
Sbjct: 703 GAGLPIISTLRGLLETGDKILRIEGIFSGTLSYIFNNFEGTRTFSNVVAEAKEAGYTEPD 762
Query: 232 PRDDLSGMDVARKALILARLLGQRISMENINVESLYPSEFGPDAMSTKDFLESGLVQLDK 291
PRDDLSG DVARK +ILAR G R+ + +I V+SL P + S+ D L D+
Sbjct: 763 PRDDLSGTDVARKVIILARESGLRLELSDIPVKSLVPEAL--RSCSSADEFMQKLPSFDQ 820
Query: 292 SIEERVKAASLKGNVLRY--VCKIESTGCQVGLEELPKNSALGRLRGSDNVVEIYSRCYE 349
+ + A G VLRY V + + +V L+ ++ +L GSDN++ + Y+
Sbjct: 821 DWDRQRDEAEAAGEVLRYVGVVDVANRKGRVELQRYKRDHPFAQLSGSDNIIAFTTSRYK 880
Query: 350 SAPLVIQXXXXXXXXXXXXVLADILDL 376
PL+++ V DIL L
Sbjct: 881 EQPLIVRGPGAGAEVTAGGVFCDILRL 907
>Os05g0295700 Similar to Homoserine dehydrogenase-like protein
Length = 100
Score = 111 bits (278), Expect = 8e-25, Method: Composition-based stats.
Identities = 57/71 (80%), Positives = 60/71 (84%), Gaps = 5/71 (7%)
Query: 171 VGAGLPVIASVTRIIASGDPV-----SRIVGSLSGTLGYVMSELEDGKRFSEVVKTAKSL 225
VGAGLPVIASVT IIASGDPV S IVGSLSGTLGYVMSELEDGKRFSEVVKTA+SL
Sbjct: 7 VGAGLPVIASVTHIIASGDPVCLLWFSHIVGSLSGTLGYVMSELEDGKRFSEVVKTARSL 66
Query: 226 GYTEPDPRDDL 236
GYTEP + +
Sbjct: 67 GYTEPGQKKNF 77
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,687,943
Number of extensions: 404043
Number of successful extensions: 898
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 893
Number of HSP's successfully gapped: 5
Length of query: 384
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 281
Effective length of database: 11,657,759
Effective search space: 3275830279
Effective search space used: 3275830279
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 157 (65.1 bits)