BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0435500 Os11g0435500|AK069963
         (95 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0435500  Raffinose synthase family protein                   176   5e-45
Os08g0495800  Raffinose synthase family protein                   106   4e-24
Os07g0209100  Similar to Seed imbibition protein (Fragment)        93   4e-20
Os12g0555400  Similar to Raffinose synthase protein                79   7e-16
Os04g0481100  Similar to Seed imbibition protein (Fragment)        75   1e-14
Os01g0170000  Raffinose synthase family protein                    70   3e-13

>Os11g0435500 Raffinose synthase family protein
          Length = 95

 Score =  176 bits (445), Expect = 5e-45,   Method: Compositional matrix adjust.
 Identities = 85/95 (89%), Positives = 85/95 (89%)

Query: 1  QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLPENAMTIQCE 60
          QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLPENAMTIQCE
Sbjct: 1  QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLPENAMTIQCE 60

Query: 61 XXXXXXXXXXWVKDVGTAWTTMTLLSSCSAPRSPQ 95
                    WVKDVGTAWTTMTLLSSCSAPRSPQ
Sbjct: 61 SMSSSVRSSSWVKDVGTAWTTMTLLSSCSAPRSPQ 95



>Os08g0495800 Raffinose synthase family protein
          Length = 712

 Score =  106 bits (264), Expect = 4e-24,   Method: Compositional matrix adjust.
 Identities = 49/51 (96%), Positives = 49/51 (96%)

Query: 1   QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLP 51
            SVHPMAEYHAAARAV GCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLP
Sbjct: 490 HSVHPMAEYHAAARAVGGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLP 540



>Os07g0209100 Similar to Seed imbibition protein (Fragment)
          Length = 763

 Score = 93.2 bits (230), Expect = 4e-20,   Method: Composition-based stats.
 Identities = 39/51 (76%), Positives = 46/51 (90%)

Query: 1   QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLP 51
            S+HP AEYH AARA+ GC IYVSDKPGNH+F+LL+KLVLPDGS+LRA+LP
Sbjct: 492 HSLHPAAEYHGAARAIGGCPIYVSDKPGNHNFDLLRKLVLPDGSVLRARLP 542



>Os12g0555400 Similar to Raffinose synthase protein
          Length = 198

 Score = 79.0 bits (193), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 35/51 (68%), Positives = 41/51 (80%)

Query: 1   QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAKLP 51
           QS+HP A+YH AA A+ GC IYVSDKPGNH+  LL+KLVLP GS LR +LP
Sbjct: 60  QSLHPAAKYHDAAMAIGGCPIYVSDKPGNHNLELLRKLVLPSGSGLRVRLP 110



>Os04g0481100 Similar to Seed imbibition protein (Fragment)
          Length = 663

 Score = 74.7 bits (182), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 34/49 (69%), Positives = 39/49 (79%)

Query: 1   QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAK 49
            S H  AE+H AARA+SG  +YVSDKPG HDF++LKKLVLPDG ILR K
Sbjct: 395 HSKHESAEFHGAARALSGGGVYVSDKPGMHDFSVLKKLVLPDGLILRTK 443



>Os01g0170000 Raffinose synthase family protein
          Length = 338

 Score = 70.5 bits (171), Expect = 3e-13,   Method: Composition-based stats.
 Identities = 31/49 (63%), Positives = 39/49 (79%)

Query: 1   QSVHPMAEYHAAARAVSGCAIYVSDKPGNHDFNLLKKLVLPDGSILRAK 49
           QS HP A +HAA+RAVSG  +YVSD  G HDF+LL++L LPDG+ILR +
Sbjct: 74  QSTHPCAAFHAASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCE 122



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.129    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,067,957
Number of extensions: 88708
Number of successful extensions: 160
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 160
Number of HSP's successfully gapped: 6
Length of query: 95
Length of database: 17,035,801
Length adjustment: 64
Effective length of query: 31
Effective length of database: 13,694,105
Effective search space: 424517255
Effective search space used: 424517255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)