BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0495900 Os10g0495900|J100073O04
         (118 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0495900  Protein of unknown function DUF1218 family pro...   241   7e-65
Os09g0281300  Protein of unknown function DUF1218 family pro...    75   7e-15
Os04g0678200  Protein of unknown function DUF1218 family pro...    74   2e-14
Os07g0462200  Protein of unknown function DUF1218 family pro...    68   1e-12

>Os10g0495900 Protein of unknown function DUF1218 family protein
          Length = 118

 Score =  241 bits (616), Expect = 7e-65,   Method: Compositional matrix adjust.
 Identities = 118/118 (100%), Positives = 118/118 (100%)

Query: 1   ITQLSSQRIYTNFIQTIQFLHTGVLNILPDVLLLRLTFLIAESCLLAGLVQSAYHTRYRK 60
           ITQLSSQRIYTNFIQTIQFLHTGVLNILPDVLLLRLTFLIAESCLLAGLVQSAYHTRYRK
Sbjct: 1   ITQLSSQRIYTNFIQTIQFLHTGVLNILPDVLLLRLTFLIAESCLLAGLVQSAYHTRYRK 60

Query: 61  VFFENPPDCETVRRGTFGAGAAFSLITCVLTGAYYFYFSKSRVSYARREATIGMSPYS 118
           VFFENPPDCETVRRGTFGAGAAFSLITCVLTGAYYFYFSKSRVSYARREATIGMSPYS
Sbjct: 61  VFFENPPDCETVRRGTFGAGAAFSLITCVLTGAYYFYFSKSRVSYARREATIGMSPYS 118



>Os09g0281300 Protein of unknown function DUF1218 family protein
          Length = 190

 Score = 75.5 bits (184), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 36/87 (41%), Positives = 56/87 (64%), Gaps = 4/87 (4%)

Query: 36  LTFLIAESCLLAGLVQSAYHTRYRKVFFENPPDCETVRRGTFGAGAAFSLITCVLTGAYY 95
           +TF+IAESCL+AG  ++AYHTRYR + +     CE++R+G F AGA F + T +L   +Y
Sbjct: 104 VTFMIAESCLIAGATKNAYHTRYRHMVYVGSWTCESLRKGVFIAGAVFVVFTMILNVYFY 163

Query: 96  FYFSKSRVSYARR----EATIGMSPYS 118
            Y++KS    A++       +GM+ Y+
Sbjct: 164 MYYTKSTSQAAKKINKTTPNVGMTGYA 190



>Os04g0678200 Protein of unknown function DUF1218 family protein
          Length = 183

 Score = 74.3 bits (181), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 34/82 (41%), Positives = 52/82 (63%)

Query: 32  LLLRLTFLIAESCLLAGLVQSAYHTRYRKVFFENPPDCETVRRGTFGAGAAFSLITCVLT 91
           ++   TF+IAE CLLAG V++AYHT+Y  +    PP C  +R+G F AGAAF+ +T +  
Sbjct: 95  IVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGVFAAGAAFTFLTALFA 154

Query: 92  GAYYFYFSKSRVSYARREATIG 113
             +Y +F+K+R + A     +G
Sbjct: 155 ELHYLFFAKARHAAAVPPPIVG 176



>Os07g0462200 Protein of unknown function DUF1218 family protein
          Length = 185

 Score = 67.8 bits (164), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 45/90 (50%), Positives = 58/90 (64%), Gaps = 3/90 (3%)

Query: 32  LLLRLTFLIAESCLLAGLVQSAYHTRYRKVFFENPPDCETVRRGTFGAGAAFSLITCVLT 91
           L   LTFLIAE+CL+AG +++AYHTRYR +F      CETVR+G F AGAAF+  T +L+
Sbjct: 96  LFAWLTFLIAEACLMAGSIRNAYHTRYRGMFVGESVSCETVRKGVFAAGAAFTFFTAILS 155

Query: 92  GAYYFYFSKSRVSYARRE---ATIGMSPYS 118
             YY  +SKSR +        + IGM  YS
Sbjct: 156 EFYYVSYSKSRDAAGGAPYGGSNIGMGTYS 185



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.140    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,549,610
Number of extensions: 124804
Number of successful extensions: 280
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 281
Number of HSP's successfully gapped: 4
Length of query: 118
Length of database: 17,035,801
Length adjustment: 85
Effective length of query: 33
Effective length of database: 12,597,611
Effective search space: 415721163
Effective search space used: 415721163
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)