BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0198900 Os10g0198900|J075038P03
         (94 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0198900  Conserved hypothetical protein                      193   2e-50
Os10g0199500  Protein of unknown function DUF6, transmembran...   130   2e-31
Os08g0402800  Protein of unknown function DUF6, transmembran...   123   3e-29
Os10g0197700  Protein of unknown function DUF6, transmembran...    79   5e-16
Os12g0288000  Protein of unknown function DUF6, transmembran...    74   2e-14

>Os10g0198900 Conserved hypothetical protein
          Length = 94

 Score =  193 bits (491), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 94/94 (100%), Positives = 94/94 (100%)

Query: 1  MDMYEHDRLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTK 60
          MDMYEHDRLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTK
Sbjct: 1  MDMYEHDRLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTK 60

Query: 61 SATNQLRGTIFLAGSSLTFACWYLVQVIIYKHCF 94
          SATNQLRGTIFLAGSSLTFACWYLVQVIIYKHCF
Sbjct: 61 SATNQLRGTIFLAGSSLTFACWYLVQVIIYKHCF 94



>Os10g0199500 Protein of unknown function DUF6, transmembrane domain containing
          protein
          Length = 222

 Score =  130 bits (327), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 62/81 (76%), Positives = 69/81 (85%)

Query: 6  HDRLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTKSATNQ 65
          + RLETF++ S  G+LKIVGVL+SVGGTMLVSLYKGKSLHLWNSILQHQ E  TK+ATNQ
Sbjct: 19 YGRLETFEIGSIVGVLKIVGVLLSVGGTMLVSLYKGKSLHLWNSILQHQNEPATKTATNQ 78

Query: 66 LRGTIFLAGSSLTFACWYLVQ 86
          LRGTI L  SS  +ACWYLVQ
Sbjct: 79 LRGTILLVASSFAYACWYLVQ 99



>Os08g0402800 Protein of unknown function DUF6, transmembrane domain containing
           protein
          Length = 347

 Score =  123 bits (309), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 60/84 (71%), Positives = 69/84 (82%), Gaps = 2/84 (2%)

Query: 8   RLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTKSATNQLR 67
           RLE FKLRS  G+LKI  +L+S+GGTML+SLYKGKSLHLW+SI+QHQ E   KSATNQLR
Sbjct: 140 RLEAFKLRSIPGVLKIASILLSIGGTMLISLYKGKSLHLWDSIIQHQNEH--KSATNQLR 197

Query: 68  GTIFLAGSSLTFACWYLVQVIIYK 91
           GTI L GSS TFACW+L+Q  I K
Sbjct: 198 GTILLVGSSFTFACWFLIQSKILK 221



>Os10g0197700 Protein of unknown function DUF6, transmembrane domain containing
           protein
          Length = 369

 Score = 79.3 bits (194), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 41/81 (50%), Positives = 52/81 (64%), Gaps = 2/81 (2%)

Query: 8   RLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLW--NSILQHQKEQQTKSATNQ 65
           R E   +RS  G++K+VG LV VGGT+++SLYKGK LHLW  N I  H  +  T    + 
Sbjct: 122 RKEPLNMRSIVGIIKVVGALVCVGGTIIISLYKGKVLHLWPTNIIGYHPSKAATAFGHHH 181

Query: 66  LRGTIFLAGSSLTFACWYLVQ 86
           +RGTI LA S L+ A WY VQ
Sbjct: 182 IRGTILLAISCLSLAVWYTVQ 202



>Os12g0288000 Protein of unknown function DUF6, transmembrane domain containing
           protein
          Length = 373

 Score = 74.3 bits (181), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 37/94 (39%), Positives = 59/94 (62%), Gaps = 9/94 (9%)

Query: 8   RLETFKLRSKAGLLKIVGVLVSVGGTMLVSLYKGKSLHLWNSILQHQKEQQTKSAT---- 63
           RLE  KL++++G++K++G ++ V GTM+VSLY+GK LHLW + L    + +    +    
Sbjct: 127 RLEKLKLKTRSGMIKVIGTVICVVGTMVVSLYRGKLLHLWPTHLLKPAQLRAIGGSDDST 186

Query: 64  -----NQLRGTIFLAGSSLTFACWYLVQVIIYKH 92
                N L GT+FL GS L++A W++VQ  + K 
Sbjct: 187 FPTHHNMLIGTLFLCGSCLSYAFWFIVQAKVNKE 220



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,625,106
Number of extensions: 80172
Number of successful extensions: 284
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 286
Number of HSP's successfully gapped: 5
Length of query: 94
Length of database: 17,035,801
Length adjustment: 63
Effective length of query: 31
Effective length of database: 13,746,319
Effective search space: 426135889
Effective search space used: 426135889
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)