BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os10g0147200 Os10g0147200|AK111104
(102 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os10g0147200 Similar to Thaumatin-like protein 135 8e-33
Os03g0244200 Similar to Thaumatin-like protein 91 2e-19
Os06g0716200 Thaumatin, pathogenesis-related family protein 74 2e-14
Os09g0536300 Similar to Thaumatin-like protein 73 4e-14
Os03g0243900 Similar to Thaumatin-like protein 66 6e-12
>Os10g0147200 Similar to Thaumatin-like protein
Length = 102
Score = 135 bits (339), Expect = 8e-33, Method: Compositional matrix adjust.
Identities = 65/65 (100%), Positives = 65/65 (100%)
Query: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSSGQNPQAAGLQQLND 60
EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSSGQNPQAAGLQQLND
Sbjct: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSSGQNPQAAGLQQLND 60
Query: 61 TMVYF 65
TMVYF
Sbjct: 61 TMVYF 65
>Os03g0244200 Similar to Thaumatin-like protein
Length = 332
Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 45/65 (69%), Positives = 51/65 (78%), Gaps = 1/65 (1%)
Query: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSSGQNPQAAGLQQLND 60
+FFK ACPRAYSYAYDDSTSTFTC AG T+YAITFCP+ + S G+NPQAAG+ ND
Sbjct: 232 QFFKNACPRAYSYAYDDSTSTFTCTAG-TNYAITFCPSTTSGKYSGGENPQAAGVPSTND 290
Query: 61 TMVYF 65
TMV
Sbjct: 291 TMVVL 295
>Os06g0716200 Thaumatin, pathogenesis-related family protein
Length = 316
Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 38/64 (59%), Positives = 44/64 (68%), Gaps = 2/64 (3%)
Query: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSSGQNPQAAGLQQLND 60
FFK ACPRAYSYAYDD+TSTFTCAA Y + FCP +S+KS G NP+A GL
Sbjct: 225 RFFKGACPRAYSYAYDDATSTFTCAAAGGGYDVVFCPGM-SSLKSGG-NPEAVGLPPTYS 282
Query: 61 TMVY 64
TM +
Sbjct: 283 TMAF 286
>Os09g0536300 Similar to Thaumatin-like protein
Length = 330
Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 37/70 (52%), Positives = 47/70 (67%), Gaps = 5/70 (7%)
Query: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCP---AAPTSVKSSGQNPQAA--GL 55
+FFK ACPRAYSYAYDD+TSTFTCA+G Y + FCP + +SV NP A+ GL
Sbjct: 211 QFFKNACPRAYSYAYDDATSTFTCASGTASYLVVFCPIISSLKSSVGGGATNPSASGTGL 270
Query: 56 QQLNDTMVYF 65
+NDT+ +
Sbjct: 271 PLINDTVSFL 280
>Os03g0243900 Similar to Thaumatin-like protein
Length = 336
Score = 65.9 bits (159), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 32/46 (69%), Positives = 37/46 (80%), Gaps = 1/46 (2%)
Query: 1 EFFKAACPRAYSYAYDDSTSTFTCAAGATDYAITFCPAAPTSVKSS 46
+ FK+ACPR+YSYAYDD TSTFTC AG DY ITFCP A S+KS+
Sbjct: 210 QVFKSACPRSYSYAYDDPTSTFTC-AGGRDYTITFCPVATPSLKSA 254
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.127 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,134,281
Number of extensions: 61060
Number of successful extensions: 277
Number of sequences better than 1.0e-10: 8
Number of HSP's gapped: 275
Number of HSP's successfully gapped: 8
Length of query: 102
Length of database: 17,035,801
Length adjustment: 70
Effective length of query: 32
Effective length of database: 13,380,821
Effective search space: 428186272
Effective search space used: 428186272
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)