BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0567400 Os09g0567400|AK072521
         (149 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0567400  Similar to Histidine-containing phosphotransfe...   303   4e-83
Os08g0557700  Similar to Histidine-containing phosphotransfe...   213   4e-56
Os05g0186100  Hpt domain containing protein                        99   2e-21
Os01g0743800  Similar to Histidine-containing phosphotransfe...    98   3e-21

>Os09g0567400 Similar to Histidine-containing phosphotransfer protein
          Length = 149

 Score =  303 bits (775), Expect = 4e-83,   Method: Compositional matrix adjust.
 Identities = 149/149 (100%), Positives = 149/149 (100%)

Query: 1   MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE 60
           MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE
Sbjct: 1   MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE 60

Query: 61  IATLLEQPVVNFDKVDAYVHQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVV 120
           IATLLEQPVVNFDKVDAYVHQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVV
Sbjct: 61  IATLLEQPVVNFDKVDAYVHQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVV 120

Query: 121 RNDFYDLRNKFQTMLQLEQQIQAYDPKQQ 149
           RNDFYDLRNKFQTMLQLEQQIQAYDPKQQ
Sbjct: 121 RNDFYDLRNKFQTMLQLEQQIQAYDPKQQ 149



>Os08g0557700 Similar to Histidine-containing phosphotransfer protein
          Length = 147

 Score =  213 bits (542), Expect = 4e-56,   Method: Compositional matrix adjust.
 Identities = 106/148 (71%), Positives = 124/148 (83%), Gaps = 1/148 (0%)

Query: 1   MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE 60
           MAAAAL  QL AL+++MF+ GL+D+QFQQLQMLQD    P FVSEVVTLFCDD +RII E
Sbjct: 1   MAAAALTSQLNALVNNMFAMGLLDDQFQQLQMLQDSTA-PDFVSEVVTLFCDDGERIICE 59

Query: 61  IATLLEQPVVNFDKVDAYVHQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVV 120
           ++  LE+P V+FD+VD+YVHQLKGSSASVGAQKVK TC+QFR+FCQ +SRDGCL  L +V
Sbjct: 60  LSRQLEKPNVDFDRVDSYVHQLKGSSASVGAQKVKNTCIQFREFCQQRSRDGCLKTLDLV 119

Query: 121 RNDFYDLRNKFQTMLQLEQQIQAYDPKQ 148
           R +FYDLRNKFQ MLQLEQQIQA  PK 
Sbjct: 120 RTEFYDLRNKFQAMLQLEQQIQACYPKH 147



>Os05g0186100 Hpt domain containing protein
          Length = 152

 Score = 98.6 bits (244), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 53/146 (36%), Positives = 88/146 (60%), Gaps = 2/146 (1%)

Query: 1   MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE 60
           M  + LR Q+  +  ++F QG +DEQF QL+ LQDE  +P FV EV  LF  D+ R++  
Sbjct: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDES-SPNFVEEVAALFFKDSSRLLTN 59

Query: 61  IATLLEQPVVNFDKVDAYVHQLKGS-SASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAV 119
           I   +++   +F ++D+ V QLKGS S+S+GA ++K  C  F+  C D++ +GC  +L  
Sbjct: 60  IEQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQK 119

Query: 120 VRNDFYDLRNKFQTMLQLEQQIQAYD 145
           ++ +   L+ K ++  QL +Q+   D
Sbjct: 120 MKREHATLKQKLESYFQLLRQVGPRD 145



>Os01g0743800 Similar to Histidine-containing phosphotransfer protein 4
          Length = 187

 Score = 97.8 bits (242), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 50/121 (41%), Positives = 77/121 (63%), Gaps = 1/121 (0%)

Query: 20  QGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINEIATLLEQPVVNFDKVDAYV 79
           +G +DEQF Q++ LQDE  +P FV EVVTLF  D+ R+++ I   LE+   +F++ D Y+
Sbjct: 56  KGYLDEQFCQVEDLQDEA-SPNFVEEVVTLFFKDSGRLMSNIEQALEKYPRDFNRWDTYM 114

Query: 80  HQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVVRNDFYDLRNKFQTMLQLEQ 139
            QLKGS +S+GA ++K  CM FR  C   + +GC+ +   V+ +   LR K ++  QL +
Sbjct: 115 QQLKGSCSSIGASRMKNECMSFRDSCGQGNVEGCMRSFQKVKREHAVLRQKLESYFQLLR 174

Query: 140 Q 140
           Q
Sbjct: 175 Q 175



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,410,351
Number of extensions: 154239
Number of successful extensions: 359
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 356
Number of HSP's successfully gapped: 4
Length of query: 149
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 57
Effective length of database: 12,232,113
Effective search space: 697230441
Effective search space used: 697230441
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 151 (62.8 bits)