BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os09g0548300 Os09g0548300|J013000L15
(98 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os09g0548300 Conserved hypothetical protein 155 6e-39
Os12g0106600 Multi antimicrobial extrusion protein MatE fam... 75 1e-14
Os02g0833100 Conserved hypothetical protein 69 1e-12
Os10g0206800 Multi antimicrobial extrusion protein MatE fam... 63 5e-11
>Os09g0548300 Conserved hypothetical protein
Length = 98
Score = 155 bits (392), Expect = 6e-39, Method: Compositional matrix adjust.
Identities = 80/98 (81%), Positives = 80/98 (81%)
Query: 1 MSRYLGMFLLLWSLSKRAVLLPPKIEDLDFVGYIKSGGMLLGRTLSVLITMTLGTAMAAR 60
MSRYLGMFLLLWSLSKRAVLLPPKIEDLDFVGYIKSGGMLLGRTLSVLITMTLGTAMAAR
Sbjct: 1 MSRYLGMFLLLWSLSKRAVLLPPKIEDLDFVGYIKSGGMLLGRTLSVLITMTLGTAMAAR 60
Query: 61 QGTIAMAAHQICLQXXXXXXXXXXXXXXXXXXQLSFVM 98
QGTIAMAAHQICLQ QLSFVM
Sbjct: 61 QGTIAMAAHQICLQVWLAVSLLSDALAVSAQVQLSFVM 98
>Os12g0106600 Multi antimicrobial extrusion protein MatE family protein
Length = 558
Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 33/73 (45%), Positives = 52/73 (71%)
Query: 2 SRYLGMFLLLWSLSKRAVLLPPKIEDLDFVGYIKSGGMLLGRTLSVLITMTLGTAMAARQ 61
S YL F+LLW L+ + VL I D + Y+KSG +L+ RT++V++T T+ T++AAR+
Sbjct: 317 SEYLTAFILLWKLNSKIVLFSWNIVSGDIIRYLKSGALLIARTIAVVLTFTVSTSLAARE 376
Query: 62 GTIAMAAHQICLQ 74
G++ MA ++ICLQ
Sbjct: 377 GSVPMAGYEICLQ 389
>Os02g0833100 Conserved hypothetical protein
Length = 106
Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 32/38 (84%), Positives = 36/38 (94%)
Query: 37 GGMLLGRTLSVLITMTLGTAMAARQGTIAMAAHQICLQ 74
GGMLLGRTLS+L+TMT+GT+MAARQG AMAAHQICLQ
Sbjct: 10 GGMLLGRTLSILLTMTIGTSMAARQGPTAMAAHQICLQ 47
>Os10g0206800 Multi antimicrobial extrusion protein MatE family protein
Length = 537
Score = 63.2 bits (152), Expect = 5e-11, Method: Compositional matrix adjust.
Identities = 31/74 (41%), Positives = 46/74 (62%)
Query: 1 MSRYLGMFLLLWSLSKRAVLLPPKIEDLDFVGYIKSGGMLLGRTLSVLITMTLGTAMAAR 60
+S+Y +LLW L LLPP + + F ++K+G +LL R ++ +TL +MAAR
Sbjct: 286 ISQYFIASILLWRLRLHVDLLPPSFKHMQFSRFLKNGFLLLARVIAATCCVTLSASMAAR 345
Query: 61 QGTIAMAAHQICLQ 74
G++ MAA QICLQ
Sbjct: 346 LGSVPMAAFQICLQ 359
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.330 0.140 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,350,122
Number of extensions: 64701
Number of successful extensions: 187
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 187
Number of HSP's successfully gapped: 4
Length of query: 98
Length of database: 17,035,801
Length adjustment: 67
Effective length of query: 31
Effective length of database: 13,537,463
Effective search space: 419661353
Effective search space used: 419661353
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 149 (62.0 bits)