BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0527500 Os09g0527500|AK110546
         (235 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0527500  Similar to MEG5                                     342   1e-94
Os09g0527100  Similar to RNA-binding protein                      248   3e-66
Os08g0547000  RNA-binding region RNP-1 (RNA recognition moti...   113   1e-25
Os06g0127500  Similar to MEG5                                     101   4e-22

>Os09g0527500 Similar to MEG5
          Length = 235

 Score =  342 bits (877), Expect = 1e-94,   Method: Compositional matrix adjust.
 Identities = 169/222 (76%), Positives = 169/222 (76%)

Query: 1   MAAESYWQYQYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MAAESYWQYQY                                                 
Sbjct: 1   MAAESYWQYQYAAVDPRQQAPAPVPVPTPALMWQQQQQQAGYQPAVAPVAPPMAAPPLPA 60

Query: 61  XXXXSFKRQRPEYFDMPSGQGNSLFHEAMSPHYVHRLNNQMSYAAGANQSAIPLGGMATY 120
               SFKRQRPEYFDMPSGQGNSLFHEAMSPHYVHRLNNQMSYAAGANQSAIPLGGMATY
Sbjct: 61  GPPPSFKRQRPEYFDMPSGQGNSLFHEAMSPHYVHRLNNQMSYAAGANQSAIPLGGMATY 120

Query: 121 SAGMDSGNHGATITESRTLYVEGLPSNCTKREVAHIFRPFSGFREVRLVNKEIRHAGSCN 180
           SAGMDSGNHGATITESRTLYVEGLPSNCTKREVAHIFRPFSGFREVRLVNKEIRHAGSCN
Sbjct: 121 SAGMDSGNHGATITESRTLYVEGLPSNCTKREVAHIFRPFSGFREVRLVNKEIRHAGSCN 180

Query: 181 LLCFVDFSSPPEARAALETLQGYKFDEHDHESSNLRIQFSLT 222
           LLCFVDFSSPPEARAALETLQGYKFDEHDHESSNLRIQFSLT
Sbjct: 181 LLCFVDFSSPPEARAALETLQGYKFDEHDHESSNLRIQFSLT 222



>Os09g0527100 Similar to RNA-binding protein
          Length = 149

 Score =  248 bits (632), Expect = 3e-66,   Method: Compositional matrix adjust.
 Identities = 122/136 (89%), Positives = 123/136 (90%), Gaps = 2/136 (1%)

Query: 89  MSPHYVHRLNNQ--MSYAAGANQSAIPLGGMATYSAGMDSGNHGATITESRTLYVEGLPS 146
           MSP Y H LN+Q  MSY AG NQSAIPLGGMA YS GMDSG HGAT TESRTLYVEGLPS
Sbjct: 1   MSPLYSHHLNDQLQMSYVAGTNQSAIPLGGMARYSVGMDSGIHGATRTESRTLYVEGLPS 60

Query: 147 NCTKREVAHIFRPFSGFREVRLVNKEIRHAGSCNLLCFVDFSSPPEARAALETLQGYKFD 206
           NCTKREVAHIFRPFSGFREVRLVNKE RHAGSCNLLCFVDFSSPPEARAALETLQGYKFD
Sbjct: 61  NCTKREVAHIFRPFSGFREVRLVNKESRHAGSCNLLCFVDFSSPPEARAALETLQGYKFD 120

Query: 207 EHDHESSNLRIQFSLT 222
           EHDHESSNLR QFSLT
Sbjct: 121 EHDHESSNLRTQFSLT 136



>Os08g0547000 RNA-binding region RNP-1 (RNA recognition motif) domain containing
           protein
          Length = 294

 Score =  113 bits (283), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 72/175 (41%), Positives = 89/175 (50%), Gaps = 38/175 (21%)

Query: 66  FKRQRPEYFDMPSGQGNSLFH---------------EAMSPHYVHRLNNQMSYAAGA--N 108
            KR RP+Y D+P+GQ  + ++               EA+   Y   L N M   A    N
Sbjct: 61  LKRARPDYGDVPAGQDMTGYYPRETDRTGYHALRENEAIGASYDRYLRNGMPSVAATETN 120

Query: 109 QSAI------------PLGGMATYSAGMDSGNHG--------ATITESRTLYVEGLPSNC 148
           +  +            P+        GMDS   G             S TLYVEGLP+NC
Sbjct: 121 RPVVGGMGGMGGMGGYPVDDRRMIGVGMDSRGMGYGARPEPPLPADASSTLYVEGLPANC 180

Query: 149 TKREVAHIFRPFSGFREVRLVNKEIRH-AGSCNLLCFVDFSSPPEARAALETLQG 202
           T+REV+HIFRPF GFREVRLVNKE RH  G  ++LCFVDF +P +A  ALE LQG
Sbjct: 181 TRREVSHIFRPFVGFREVRLVNKESRHPGGDPHVLCFVDFDNPAQATLALEALQG 235



>Os06g0127500 Similar to MEG5
          Length = 293

 Score =  101 bits (252), Expect = 4e-22,   Method: Compositional matrix adjust.
 Identities = 57/137 (41%), Positives = 74/137 (54%), Gaps = 31/137 (22%)

Query: 115 GGMATYSAGMDSGNHGATITE--SRTLYVEGLPSNCTKREVAHI---------------- 156
           GGM   + G   G     +    S TL++EG+P++C +REV+ I                
Sbjct: 139 GGMEARNIGFGGGMPEPPLPPDASNTLFIEGIPTDCARREVSRILKSHAFFCLSLIFHLH 198

Query: 157 ------------FRPFSGFREVRLVNKEIRH-AGSCNLLCFVDFSSPPEARAALETLQGY 203
                       FRPF GFREVRLV+KE RH  G   LLCFVDF +  +A  A++ LQGY
Sbjct: 199 KFCWSFTFPQDIFRPFVGFREVRLVSKEARHPGGDPILLCFVDFETASQAAIAMDALQGY 258

Query: 204 KFDEHDHESSNLRIQFS 220
           KFDEHD  S +LR+QF+
Sbjct: 259 KFDEHDRNSPHLRLQFA 275



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.131    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,841,646
Number of extensions: 216097
Number of successful extensions: 611
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 607
Number of HSP's successfully gapped: 4
Length of query: 235
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 137
Effective length of database: 11,918,829
Effective search space: 1632879573
Effective search space used: 1632879573
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)