BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0511500 Os09g0511500|AK060504
         (404 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0511500  Zinc finger, RING-type domain containing protein    783   0.0  
Os08g0539300  Zinc finger, RING-type domain containing protein    280   2e-75
Os05g0154600  Similar to VIP2 protein                             135   5e-32
Os01g0350900  Similar to VIP2 protein                             134   2e-31

>Os09g0511500 Zinc finger, RING-type domain containing protein
          Length = 404

 Score =  783 bits (2022), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 379/404 (93%), Positives = 379/404 (93%)

Query: 1   MAFAAEPMDLEVSPDSSPXXXXXXXVCSICLDAVACGDGVAARSTARLQCGHEFHLDCIG 60
           MAFAAEPMDLEVSPDSSP       VCSICLDAVACGDGVAARSTARLQCGHEFHLDCIG
Sbjct: 1   MAFAAEPMDLEVSPDSSPAAAAAAAVCSICLDAVACGDGVAARSTARLQCGHEFHLDCIG 60

Query: 61  SAFNAKGVMQCPNCRNIEKGHWLYGNESQPCSHSDTGDWLNGETFDYPFSFEFGWCPFNT 120
           SAFNAKGVMQCPNCRNIEKGHWLYGNESQPCSHSDTGDWLNGETFDYPFSFEFGWCPFNT
Sbjct: 61  SAFNAKGVMQCPNCRNIEKGHWLYGNESQPCSHSDTGDWLNGETFDYPFSFEFGWCPFNT 120

Query: 121 PLTSVFGESESEPNPFLEYIGSLHGFHHPMYAPSSSTASTESIPFHQRPTGTEGHATTDL 180
           PLTSVFGESESEPNPFLEYIGSLHGFHHPMYAPSSSTASTESIPFHQRPTGTEGHATTDL
Sbjct: 121 PLTSVFGESESEPNPFLEYIGSLHGFHHPMYAPSSSTASTESIPFHQRPTGTEGHATTDL 180

Query: 181 RNTQVFNESEPRNHEREQQYLGSVQMPGTLNHSTAPFGIGMPRYDGGNQQRLRPHMHDNS 240
           RNTQVFNESEPRNHEREQQYLGSVQMPGTLNHSTAPFGIGMPRYDGGNQQRLRPHMHDNS
Sbjct: 181 RNTQVFNESEPRNHEREQQYLGSVQMPGTLNHSTAPFGIGMPRYDGGNQQRLRPHMHDNS 240

Query: 241 LFHRPTARRASNLAHLRSLTAASETRGHGHGMTSHAVQQTIPSSMASNPQPPATRRVRPR 300
           LFHRPTARRASNLAHLRSLTAASETRGHGHGMTSHAVQQTIPSSMASNPQPPATRRVRPR
Sbjct: 241 LFHRPTARRASNLAHLRSLTAASETRGHGHGMTSHAVQQTIPSSMASNPQPPATRRVRPR 300

Query: 301 XXXXXXXXXXXXXXXXXXPHDFPLTETASTTNGNIRNGVGAPRHANQSYSWSSETFWPQT 360
                             PHDFPLTETASTTNGNIRNGVGAPRHANQSYSWSSETFWPQT
Sbjct: 301 ALSITSFIAASSSAEIRAPHDFPLTETASTTNGNIRNGVGAPRHANQSYSWSSETFWPQT 360

Query: 361 GEPHWWSPMAPVHNRSYDNFSGRSATELLSIYGAQNGLPTPRFL 404
           GEPHWWSPMAPVHNRSYDNFSGRSATELLSIYGAQNGLPTPRFL
Sbjct: 361 GEPHWWSPMAPVHNRSYDNFSGRSATELLSIYGAQNGLPTPRFL 404



>Os08g0539300 Zinc finger, RING-type domain containing protein
          Length = 424

 Score =  280 bits (715), Expect = 2e-75,   Method: Compositional matrix adjust.
 Identities = 172/389 (44%), Positives = 215/389 (55%), Gaps = 36/389 (9%)

Query: 27  CSICLDAVACGDGVAARSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRNIEKGHWLYGN 86
           CSICLD V  G     RS ARLQCGHEFHLDCIGSAFNAKG MQCPNCR IEKG WLY +
Sbjct: 25  CSICLDPVVAG----GRSVARLQCGHEFHLDCIGSAFNAKGAMQCPNCRKIEKGRWLYAS 80

Query: 87  ESQPCSHSDTGDWLNGETFDYPFSFEFG--WCPFN--TPLTSVFGESESEPNPF------ 136
              P    D G W+ GET+D      FG  WCPF+  T L SVF E E+E   +      
Sbjct: 81  GHHPSPDIDIGGWVTGETYDITSDIPFGFQWCPFSGFTQLASVFEEGEAEQTSYHTVGDH 140

Query: 137 ---------LEYIGSLHGFHHPMYAPSSSTASTESIPFHQRPTGTEGHATTDLRNTQVFN 187
                      Y+ +L GF HP++ PSSS++  E+  FH+ PT  EGHA  DL NTQVF+
Sbjct: 141 SNAASSSLVCPYL-ALRGFLHPVHVPSSSSSGAENSSFHRHPTSLEGHAAHDLSNTQVFH 199

Query: 188 ESEPRNHEREQQYLGSVQMPGTLNHSTAPFGIGMPRYDGGNQQRLRPHMHDNSLFHRPTA 247
            +E RNH+ + +Y+ ++ + G  +HS APFGIG+PRYD  +QQR RP+ H   L HRPT 
Sbjct: 200 ATESRNHDNDHRYMSNLPVSGIPDHSVAPFGIGLPRYDSSSQQRTRPYAHHRPLVHRPTP 259

Query: 248 RRASNLAH-LRSLTAA-SETRGHGHGMTSHAVQQTIPSSMASNPQPPATRRVRPRXXXXX 305
           R  SN+   L S+ A  +ETRGHGHG   H  QQ++  S+ S+P PP +RRVRPR     
Sbjct: 260 RNGSNMVTPLGSVPAVMAETRGHGHGARGHMYQQSM-HSLQSSPFPPTSRRVRPRALTIT 318

Query: 306 XXXXXXXXXXXXXPHDFPLTETASTTNGNIRNGVGAPRHANQSYSWSSETF----W-PQT 360
                         H F           N  +G    R  ++ Y W  E F    W P  
Sbjct: 319 SFIAASSSAEIGGHHGF----APPVNRSNSSDGEAVSRPVDRPYGWGQEGFTPFPWIPAD 374

Query: 361 GEPHWWSPMAPVHNRSYDNFSGRSATELL 389
           GE HWW    P+ N ++  F+ R   E +
Sbjct: 375 GESHWWGTFNPMQNHTHGGFTRRPTGERM 403



>Os05g0154600 Similar to VIP2 protein
          Length = 436

 Score =  135 bits (341), Expect = 5e-32,   Method: Compositional matrix adjust.
 Identities = 77/146 (52%), Positives = 91/146 (62%), Gaps = 10/146 (6%)

Query: 27  CSICLDAVACGDGVAARSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRNIEKGHWLYGN 86
           CSICLDAV    G   RSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCR IEKG+WLY N
Sbjct: 32  CSICLDAVVAAGG--ERSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRKIEKGNWLYAN 89

Query: 87  ESQPCSHSDTGDWLNGE-TFDYPFS---FEFGWCPFN--TPLTSVFGESESE-PNPFLEY 139
            S+P    +  +W + E  +D  +S   F F WCPF     L S F E ES  P  F ++
Sbjct: 90  GSRPTQDVNMDEWAHDEDLYDVSYSEMPFRFHWCPFGRLAQLPSFFEEGESSPPVTFHDF 149

Query: 140 IGSLHGFHHPMYAPSSSTASTESIPF 165
           +G  H F   + A SS+  +    P+
Sbjct: 150 MGQ-HMFTEHVAAVSSAPGAAHPCPY 174



>Os01g0350900 Similar to VIP2 protein
          Length = 374

 Score =  134 bits (336), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 75/144 (52%), Positives = 86/144 (59%), Gaps = 16/144 (11%)

Query: 27  CSICLDAVACGDGVAARSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRNIEKGHWLYGN 86
           CSICLDAV  G G   RSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCR IE+G+WLY N
Sbjct: 122 CSICLDAVVAGGG--DRSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRQIERGNWLYAN 179

Query: 87  ESQPCSHSDTGDWLNGETF----------DYPFSFEFGWCPFN--TPLTSVFGESESE-P 133
            S+P       DW + E F               F F WCP      L SVF E ES  P
Sbjct: 180 GSRPSQDVSNDDWGHDEDFYDANQPETSRSVFLPFRFQWCPIGRLAQLPSVFDEGESAPP 239

Query: 134 NPFLEYIG-SLHGFHHPMYAPSSS 156
             F +++G +    H P+ AP ++
Sbjct: 240 VTFHDFMGQNFTSEHLPVSAPGAT 263



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.131    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,246,267
Number of extensions: 675344
Number of successful extensions: 2213
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 2201
Number of HSP's successfully gapped: 4
Length of query: 404
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 301
Effective length of database: 11,657,759
Effective search space: 3508985459
Effective search space used: 3508985459
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)