BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0562700 Os08g0562700|AK064900
         (418 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0562700  Similar to Aminopeptidase                           873   0.0  
Os02g0218200  Peptidase M1, membrane alanine aminopeptidase ...    79   4e-15
Os08g0398700  Peptidase M1, membrane alanine aminopeptidase ...    77   3e-14
Os09g0362500  Peptidase M1, membrane alanine aminopeptidase ...    70   3e-12

>Os08g0562700 Similar to Aminopeptidase
          Length = 418

 Score =  873 bits (2256), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 418/418 (100%), Positives = 418/418 (100%)

Query: 1   MDLPKEIFLKEYKKPDYLFDSVNLEFQLGEDKTIVTSKIAVSPGTEGTSSPLTLHGRDLK 60
           MDLPKEIFLKEYKKPDYLFDSVNLEFQLGEDKTIVTSKIAVSPGTEGTSSPLTLHGRDLK
Sbjct: 1   MDLPKEIFLKEYKKPDYLFDSVNLEFQLGEDKTIVTSKIAVSPGTEGTSSPLTLHGRDLK 60

Query: 61  LLSIKVNGKDLKSEDYMVDSRHLTVSRPPGGTFNLEIVTEIYPQLNTSLEGLYKSTGNFC 120
           LLSIKVNGKDLKSEDYMVDSRHLTVSRPPGGTFNLEIVTEIYPQLNTSLEGLYKSTGNFC
Sbjct: 61  LLSIKVNGKDLKSEDYMVDSRHLTVSRPPGGTFNLEIVTEIYPQLNTSLEGLYKSTGNFC 120

Query: 121 TQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYPVLLSNGNLIEQGDLEGGKHYALWE 180
           TQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYPVLLSNGNLIEQGDLEGGKHYALWE
Sbjct: 121 TQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYPVLLSNGNLIEQGDLEGGKHYALWE 180

Query: 181 DPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRIWTPGQDLAKTAHAMYSLKAAMKWD 240
           DPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRIWTPGQDLAKTAHAMYSLKAAMKWD
Sbjct: 181 DPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRIWTPGQDLAKTAHAMYSLKAAMKWD 240

Query: 241 EEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNIFQSRLVLASPETATDGDYAAILGVVGH 300
           EEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNIFQSRLVLASPETATDGDYAAILGVVGH
Sbjct: 241 EEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNIFQSRLVLASPETATDGDYAAILGVVGH 300

Query: 301 EYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQEFSSDLGCRTVKRIADVSKLRTYQFPQDA 360
           EYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQEFSSDLGCRTVKRIADVSKLRTYQFPQDA
Sbjct: 301 EYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQEFSSDLGCRTVKRIADVSKLRTYQFPQDA 360

Query: 361 GPMAHPIRPHSYIKMDNFYTGEFMKRVLKLLECTRPCLELQGFERAWIFTSKGTTGKL 418
           GPMAHPIRPHSYIKMDNFYTGEFMKRVLKLLECTRPCLELQGFERAWIFTSKGTTGKL
Sbjct: 361 GPMAHPIRPHSYIKMDNFYTGEFMKRVLKLLECTRPCLELQGFERAWIFTSKGTTGKL 418



>Os02g0218200 Peptidase M1, membrane alanine aminopeptidase family protein
          Length = 878

 Score = 79.3 bits (194), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 67/235 (28%), Positives = 102/235 (43%), Gaps = 21/235 (8%)

Query: 105 LNTSLEGLYKSTGNF--------CTQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYP 156
           LN  + G Y+S             TQ E    R+     D P   A +   +E       
Sbjct: 118 LNDKMHGFYRSVYELNGEKKNMAVTQFEPADARRCFPCWDEPSFKAIFKITLEVPSE--T 175

Query: 157 VLLSNGNLIEQGDLEGGKHYALWEDPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRI 216
           V LSN  ++E+      K     E P    +YL A++ G  D  E +FTT   R   +R+
Sbjct: 176 VALSNMPVVEEKVNGLIKAVYFQETPIMS-TYLVAVIVGMFDYVE-AFTTDGTR---VRV 230

Query: 217 WTPGQDLAKTAHAMYSLKAAMK---WDEEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNI 273
           +T    + K+A   ++L+ A+K     +E F + Y L   +++ +PDF  GAMEN  L  
Sbjct: 231 YT---QVGKSAQGKFALEVAVKTLVLFKEYFAVPYPLPKMDMIAIPDFASGAMENYGLVT 287

Query: 274 FQSRLVLASPETATDGDYAAILGVVGHEYFHNWTGNRVTCRDWFQLTLKEGLTVF 328
           ++   +L   + +   +   +  VV HE  H W GN VT   W  L L EG   +
Sbjct: 288 YRETALLFDEKHSAAANKQRVAVVVAHELAHQWFGNLVTMEWWTHLWLNEGFATW 342



>Os08g0398700 Peptidase M1, membrane alanine aminopeptidase family protein
          Length = 875

 Score = 77.0 bits (188), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 68/233 (29%), Positives = 101/233 (43%), Gaps = 21/233 (9%)

Query: 105 LNTSLEGLYKSTGNF--------CTQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYP 156
           LN  + G Y+S   +         TQ EA   R+     D P   A +   +E    L  
Sbjct: 109 LNDQMRGFYRSKYEYKGESRNMAVTQFEAADARRCFPCWDEPAFKAKFKLTLEVPSEL-- 166

Query: 157 VLLSNGNLIEQGDLEGGKHYALWEDPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRI 216
           V LSN  +I++  + G      +E+     +YL A+V G  D  E S  T  G KV  R+
Sbjct: 167 VALSNMPVIKE-TVHGPLKTVYYEESPLMSTYLVAIVVGLFDYIEGS--TLEGTKV--RV 221

Query: 217 WTPGQDLAKTAHAMYSLKAAMK---WDEEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNI 273
           +T    + K+    ++L  A+K     ++ F   Y L   ++V +PDF  GAMEN  L  
Sbjct: 222 YT---QVGKSNQGKFALDVAVKSLDLFKDYFATPYPLPKLDMVAIPDFAAGAMENYGLVT 278

Query: 274 FQSRLVLASPETATDGDYAAILGVVGHEYFHNWTGNRVTCRDWFQLTLKEGLT 326
           ++   +L     ++  +   +   V HE  H W GN VT   W  L L EG  
Sbjct: 279 YRETALLYDELLSSASNKQQVAITVAHELAHQWFGNLVTMEWWTHLWLNEGFA 331



>Os09g0362500 Peptidase M1, membrane alanine aminopeptidase family protein
          Length = 870

 Score = 70.1 bits (170), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 67/235 (28%), Positives = 102/235 (43%), Gaps = 21/235 (8%)

Query: 105 LNTSLEGLYKS-------TGNFC-TQCEAEGFRKITYFQDRPDVMATYTCRIEADKTLYP 156
           LN  + G Y+S       T N   TQ EA   R+     D P   A +   +E    L  
Sbjct: 107 LNDQMRGFYRSKYEYKGETKNMAVTQFEAVDARRCFPCWDEPAFKAKFKLTLEVPSEL-- 164

Query: 157 VLLSNGNLIEQGDLEGGKHYALWEDPFKKPSYLFALVAGQLDCREDSFTTCSGRKVTLRI 216
           V LSN  +  +  + G      +E+     +YL A+V G  D  E    T  G KV  R+
Sbjct: 165 VALSNMPVACET-IAGPIKTIHYEESPLMSTYLVAIVVGLFDYVEG--VTSEGNKV--RV 219

Query: 217 WTPGQDLAKTAHAMYSLKAAMK---WDEEVFGLEYDLDLFNIVVVPDFNMGAMENKSLNI 273
           +T    + K++   ++L   +K   + ++ F   Y L   ++V +PDF  GAMEN  L  
Sbjct: 220 YT---QVGKSSQGKFALDIGVKSLNFYKDYFDTPYPLPKLDMVAIPDFAAGAMENYGLVT 276

Query: 274 FQSRLVLASPETATDGDYAAILGVVGHEYFHNWTGNRVTCRDWFQLTLKEGLTVF 328
           ++   +L   ++++      +   V HE  H W GN VT   W  L L EG   +
Sbjct: 277 YREVSLLFDEQSSSASFKQNVAITVAHELAHQWFGNLVTMEWWTHLWLNEGFATW 331



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,763,149
Number of extensions: 604425
Number of successful extensions: 1023
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1022
Number of HSP's successfully gapped: 4
Length of query: 418
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 315
Effective length of database: 11,657,759
Effective search space: 3672194085
Effective search space used: 3672194085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)