BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0249500 Os08g0249500|Os08g0249500
         (127 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0249500  Diacylglycerol kinase accessory region domain ...   263   2e-71
Os02g0787800  Diacylglycerol kinase, catalytic region domain...   199   4e-52
Os04g0634700  Similar to Diacylglycerol kinase                     87   3e-18
Os08g0178700  Similar to Calmodulin-binding diacylglycerol k...    87   3e-18
Os01g0783200  Similar to Diacylglycerol kinase                     82   8e-17

>Os08g0249500 Diacylglycerol kinase accessory region domain containing protein
          Length = 127

 Score =  263 bits (673), Expect = 2e-71,   Method: Compositional matrix adjust.
 Identities = 127/127 (100%), Positives = 127/127 (100%)

Query: 1   MLYFSVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASL 60
           MLYFSVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASL
Sbjct: 1   MLYFSVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASL 60

Query: 61  VMVELISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFVDIKKVPYPSL 120
           VMVELISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFVDIKKVPYPSL
Sbjct: 61  VMVELISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFVDIKKVPYPSL 120

Query: 121 IINGGDR 127
           IINGGDR
Sbjct: 121 IINGGDR 127



>Os02g0787800 Diacylglycerol kinase, catalytic region domain containing protein
          Length = 488

 Score =  199 bits (507), Expect = 4e-52,   Method: Compositional matrix adjust.
 Identities = 91/120 (75%), Positives = 106/120 (88%)

Query: 5   SVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASLVMVE 64
           SVR++V LNL+NY SGR+PWG+LKP+YLEK+GFVEA SDDGLLEIFGLK+GWHAS VM E
Sbjct: 367 SVRSLVVLNLYNYGSGRHPWGDLKPDYLEKKGFVEAHSDDGLLEIFGLKEGWHASFVMAE 426

Query: 65  LISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFVDIKKVPYPSLIING 124
           LI AKHIAQAAAI+ E++GGQW  AY+QMDGEPWKQPL  E ST ++I KVPYPSL+ING
Sbjct: 427 LIKAKHIAQAAAIKFEMRGGQWNRAYVQMDGEPWKQPLLQEQSTIIEINKVPYPSLMING 486



>Os04g0634700 Similar to Diacylglycerol kinase
          Length = 499

 Score = 87.0 bits (214), Expect = 3e-18,   Method: Composition-based stats.
 Identities = 40/106 (37%), Positives = 58/106 (54%)

Query: 5   SVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASLVMVE 64
           S+R+IV LNL +++ G NPWG      ++ R       DDGL+E+ G +  WH  +++  
Sbjct: 321 SIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAP 380

Query: 65  LISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFV 110
                 +AQA  IR E   G     +M++DGEPWKQPL  +  T V
Sbjct: 381 NGHGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDDDTVV 426



>Os08g0178700 Similar to Calmodulin-binding diacylglycerol kinase
          Length = 502

 Score = 87.0 bits (214), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 40/109 (36%), Positives = 60/109 (55%)

Query: 2   LYFSVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFVEAQSDDGLLEIFGLKQGWHASLV 61
           ++ S+R+IV LNL +++ G NPWG      +E+R       DD L+E+ G +  WH  ++
Sbjct: 318 IHHSIRSIVCLNLPSFSGGLNPWGTPGTRKVEERELTAPFVDDRLIEVVGFRDAWHGLVL 377

Query: 62  MVELISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPLDHEYSTFV 110
           +        +AQA  IR E   G     +M++DGEPWKQPL  +  T V
Sbjct: 378 LAPNGHGTRLAQAQRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDDDTVV 426



>Os01g0783200 Similar to Diacylglycerol kinase
          Length = 487

 Score = 82.0 bits (201), Expect = 8e-17,   Method: Compositional matrix adjust.
 Identities = 41/99 (41%), Positives = 55/99 (55%), Gaps = 1/99 (1%)

Query: 5   SVRAIVALNLHNYASGRNPWGNLKPEYLEKRGFV-EAQSDDGLLEIFGLKQGWHASLVMV 63
           S+R+IV LNL +++ G NPWG        KR  V     DDGLLEI G K  WH  +++ 
Sbjct: 311 SIRSIVCLNLPSFSGGLNPWGTPSERKQRKRDLVMPPLVDDGLLEIVGFKDAWHGLVLLS 370

Query: 64  ELISAKHIAQAAAIRLEIKGGQWRDAYMQMDGEPWKQPL 102
                  +AQA  ++ +   G    AYM++DGEPW QPL
Sbjct: 371 PKGHGTRLAQAHRVQFKFHKGATDHAYMRLDGEPWNQPL 409



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,688,314
Number of extensions: 178184
Number of successful extensions: 285
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 285
Number of HSP's successfully gapped: 5
Length of query: 127
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 38
Effective length of database: 12,388,755
Effective search space: 470772690
Effective search space used: 470772690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 150 (62.4 bits)