BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0244100 Os08g0244100|AK102977
         (361 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0244100  t-snare domain containing protein                   724   0.0  
Os03g0207200  t-snare domain containing protein                   133   2e-31
Os10g0343100  t-snare domain containing protein                   125   5e-29
Os05g0443700  t-snare domain containing protein                    89   6e-18

>Os08g0244100 t-snare domain containing protein
          Length = 361

 Score =  724 bits (1868), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 350/361 (96%), Positives = 350/361 (96%)

Query: 1   MAAAGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRERDSGANLEELSDLQRELKTAL 60
           MAAAGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRERDSGANLEELSDLQRELKTAL
Sbjct: 1   MAAAGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRERDSGANLEELSDLQRELKTAL 60

Query: 61  GTAKWQLEQFERAISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQINDYSIDNDRRG 120
           GTAKWQLEQFERAISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQINDYSIDNDRRG
Sbjct: 61  GTAKWQLEQFERAISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQINDYSIDNDRRG 120

Query: 121 LNWVKLDDEERDDLVAFLSAPAQLSQDTKKRDNTYHSPSKQKNALIGANDPRDMAAISKD 180
           LNWVKLDDEERDDLVAFLSAPAQLSQDTKKRDNTYHSPSKQKNALIGANDPRDMAAISKD
Sbjct: 121 LNWVKLDDEERDDLVAFLSAPAQLSQDTKKRDNTYHSPSKQKNALIGANDPRDMAAISKD 180

Query: 181 RHKTEALCREISNGQSEACCLAEQLNGHSTSPSSGGEHWXXXXXXXXXXXRKLSPNKVEA 240
           RHKTEALCREISNGQSEACCLAEQLNGHSTSPSSGGEHW           RKLSPNKVEA
Sbjct: 181 RHKTEALCREISNGQSEACCLAEQLNGHSTSPSSGGEHWKIDISDDKDDDRKLSPNKVEA 240

Query: 241 SSQATAFSGIMKTTESFTRVRWLRNSLWKAKSDEHLPLRYDMPNHLDWRVITLLAQRFNG 300
           SSQATAFSGIMKTTESFTRVRWLRNSLWKAKSDEHLPLRYDMPNHLDWRVITLLAQRFNG
Sbjct: 241 SSQATAFSGIMKTTESFTRVRWLRNSLWKAKSDEHLPLRYDMPNHLDWRVITLLAQRFNG 300

Query: 301 LTERSRSCFSGWKENSRVSGRMGGLHIQGPQYNTQFGRSIRITLLLVLSIFLIVPFLVYS 360
           LTERSRSCFSGWKENSRVSGRMGGLHIQGPQYNTQFGRSIRITLLLVLSIFLIVPFLVYS
Sbjct: 301 LTERSRSCFSGWKENSRVSGRMGGLHIQGPQYNTQFGRSIRITLLLVLSIFLIVPFLVYS 360

Query: 361 A 361
           A
Sbjct: 361 A 361



>Os03g0207200 t-snare domain containing protein
          Length = 364

 Score =  133 bits (335), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 117/394 (29%), Positives = 183/394 (46%), Gaps = 79/394 (20%)

Query: 4   AGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRERDSGANLEELS------------- 50
           A   ++W+KD FF AAEEVQ SAD MES+YR W+++R  G +    +             
Sbjct: 2   ATSFDRWEKDPFFPAAEEVQGSADRMESVYRIWVQDRSGGDSEAAAAAAAAAVGGGGLPA 61

Query: 51  -DLQRELKTALGTAKWQLEQFERAISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQI 109
            +L+REL TALGTAKWQL++ ERAI  ++  +S  + T AR   FV A+  +I +VE  +
Sbjct: 62  GELRRELHTALGTAKWQLDELERAIRSNDKVFSAGKDTKARHDDFVAAIGCRILEVENNL 121

Query: 110 NDYSIDNDRRGLNWVKLDDEERDDLVAFLSAPAQLSQDTKKRDNTYHSPSKQKNALIGAN 169
            + ++   R  L+W+ LD++ER+DL  FLSA +      ++RD     PS      +G  
Sbjct: 122 KESNVAEGRGALSWIDLDEDERNDLATFLSASS-----FQQRDKVVTIPS------VGDI 170

Query: 170 DPRDMAAISKDRHKTEALCREISNGQSEACCLAEQLNGHSTSPSSGGE--HWXXXXXXXX 227
           D  + AA+ K     ++     S   S A    E   GH  + S+  +   W        
Sbjct: 171 DVGNNAAMVKKDMYADSSKDSGSAELSSARVKEETHRGHRRAASAHADIGSWTM------ 224

Query: 228 XXXRKLSPNKVEASSQ--------------------ATAFSGIMKTTESFTRVRWLRNSL 267
                L PN+ E+S+                      +A     +T +    V+W   ++
Sbjct: 225 -----LCPNESESSADLPYDDKHQEPLLKIVKTCALTSALQSKPRTKKKGGSVKWA--AV 277

Query: 268 WKAKSDEHLPLRYDMPNHLDWRVITLLAQRFNGLTERSRSCFSGWKE---NSRVSGRMGG 324
            +   +E +PL   M             Q  +   ERS+SC S   E   N ++ G +G 
Sbjct: 278 DQQDVEETIPLSSQM------------GQGSDRCFERSKSCVSTCDESTYNKKLYGCLGA 325

Query: 325 LH--IQGPQYNTQFGRSIRITLLLV--LSIFLIV 354
           LH  +Q  +Y  ++GR +++ +L V  L IF+ V
Sbjct: 326 LHRRLQRSRYRIRYGRPVQLIVLAVAALLIFMCV 359



>Os10g0343100 t-snare domain containing protein
          Length = 367

 Score =  125 bits (314), Expect = 5e-29,   Method: Compositional matrix adjust.
 Identities = 114/370 (30%), Positives = 176/370 (47%), Gaps = 56/370 (15%)

Query: 4   AGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRERD--SGANLE------------EL 49
           A   ++W+KD FF AAEEVQESAD MES+YR W++ER    G  +E              
Sbjct: 2   ATSFDRWEKDPFFPAAEEVQESADRMESVYRRWLQERKEVGGGAVETAAERGGGGWGRAA 61

Query: 50  SDLQRELKTALGTAKWQLEQFERAISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQI 109
            DL+REL TALGTAKWQL++ +RAI  +       + T AR   FV A+  +I +VEK +
Sbjct: 62  GDLRRELHTALGTAKWQLDELQRAIKSNYSVVLAGKDTRARHDDFVSAIGHRILEVEKFL 121

Query: 110 NDYSIDNDRRGLNWVKLDDEERDDLVAFLSAPAQLSQDTKKRDNTYHSPSKQKNALIGAN 169
            + +    R  L+WV+LD+ ER++L  FLSA        +KRD    + +   +  +G+N
Sbjct: 122 KESNTTEGRGPLSWVRLDEGEREELAHFLSAGTY-----QKRDEVV-TITSAGDIEVGSN 175

Query: 170 DPRDMAAISKDRHKTEALCREISNGQSEACCLA---EQLNGHSTSPSSGGE--HWXXXXX 224
             R    +S D           S+G +E+  ++   E   GH  + S+  +   W     
Sbjct: 176 ARRVKKGVSIDSSND-------SSGSAESGLVSTKEETAPGHRRTASAYADIGSWTITIP 228

Query: 225 XXXXXXRKLSPNKVEASS--QATAFSGIMKTTESFTRVRWLRNSLWKAKSD-----EHLP 277
                  + S + +      ++ + S +M   +S TR++    +   A +D     E LP
Sbjct: 229 DEANGIDEQSFDDLPKVPLVKSPSSSVLMNAFQSKTRMKTKNGAKKLAGADQQDVVETLP 288

Query: 278 LRYDMPNHLDWRVITLLAQRFNGLTERSRSCFSGWKE----NSRVSGRMGGLH--IQGPQ 331
           L             +   Q F+GL +RS+SC S   +    N ++ G +G     +Q  Q
Sbjct: 289 L-----------TNSRSCQGFDGLFQRSKSCLSTSDDEDNCNKKLYGCLGAFRRLLQRSQ 337

Query: 332 YNTQFGRSIR 341
           Y  Q+GR I+
Sbjct: 338 YQVQYGRPIQ 347



>Os05g0443700 t-snare domain containing protein
          Length = 335

 Score = 89.0 bits (219), Expect = 6e-18,   Method: Compositional matrix adjust.
 Identities = 58/146 (39%), Positives = 86/146 (58%), Gaps = 11/146 (7%)

Query: 4   AGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRER----DSGANLEELSDLQ--RELK 57
           A G  +W+ D  F AAE VQ+SAD ME +YR  + ER    D  ++ +    +Q  R++ 
Sbjct: 2   AEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQYERDVC 61

Query: 58  TALGTAKWQLEQFERAISMS--NDKYSLEEGTLARRRQFVVAMEDQISQVEKQINDYSID 115
           TALGT KWQLEQFER ++ +  ++K +  E  + + RQF+ A+ +QISQVE  +   S+ 
Sbjct: 62  TALGTTKWQLEQFEREVNAAALSNKSNSRENAILQFRQFIRAIAEQISQVEDSLE--SLR 119

Query: 116 NDR-RGLNWVKLDDEERDDLVAFLSA 140
           ND  R      L + + D+L +FLS 
Sbjct: 120 NDSIRTPKHSYLTEHDGDELASFLSG 145



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.129    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,807,789
Number of extensions: 460544
Number of successful extensions: 1245
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1242
Number of HSP's successfully gapped: 4
Length of query: 361
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 259
Effective length of database: 11,709,973
Effective search space: 3032883007
Effective search space used: 3032883007
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)