BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0443700 Os05g0443700|AK071068
         (335 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0443700  t-snare domain containing protein                   658   0.0  
Os10g0343100  t-snare domain containing protein                    89   4e-18
Os08g0244100  t-snare domain containing protein                    89   6e-18
Os03g0207200  t-snare domain containing protein                    79   5e-15

>Os05g0443700 t-snare domain containing protein
          Length = 335

 Score =  658 bits (1697), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 313/313 (100%), Positives = 313/313 (100%)

Query: 1   MAEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQYERDV 60
           MAEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQYERDV
Sbjct: 1   MAEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQYERDV 60

Query: 61  CTALGTTKWQLEQFEREVNAAALSNKSNSRENAILQFRQFIRAIAEQISQVEDSLESLRN 120
           CTALGTTKWQLEQFEREVNAAALSNKSNSRENAILQFRQFIRAIAEQISQVEDSLESLRN
Sbjct: 61  CTALGTTKWQLEQFEREVNAAALSNKSNSRENAILQFRQFIRAIAEQISQVEDSLESLRN 120

Query: 121 DSIRTPKHSYLTEHDGDELASFLSGSNRKDNHVIYSTGTDEIVELKLDSVPAVNGYHSTQ 180
           DSIRTPKHSYLTEHDGDELASFLSGSNRKDNHVIYSTGTDEIVELKLDSVPAVNGYHSTQ
Sbjct: 121 DSIRTPKHSYLTEHDGDELASFLSGSNRKDNHVIYSTGTDEIVELKLDSVPAVNGYHSTQ 180

Query: 181 ERTSCEFRYSGEDVEGAAKPQCSCGENACEGDHNGSSMRGLDSDDSIGRKHHFRSKLSRK 240
           ERTSCEFRYSGEDVEGAAKPQCSCGENACEGDHNGSSMRGLDSDDSIGRKHHFRSKLSRK
Sbjct: 181 ERTSCEFRYSGEDVEGAAKPQCSCGENACEGDHNGSSMRGLDSDDSIGRKHHFRSKLSRK 240

Query: 241 YHSFMRNLWFTNRGRESFTKRRKDGEVMDSLRNGSTLPSFNLPPAGRAMYFWPELIKRRL 300
           YHSFMRNLWFTNRGRESFTKRRKDGEVMDSLRNGSTLPSFNLPPAGRAMYFWPELIKRRL
Sbjct: 241 YHSFMRNLWFTNRGRESFTKRRKDGEVMDSLRNGSTLPSFNLPPAGRAMYFWPELIKRRL 300

Query: 301 SKFKCSTHHKHLQ 313
           SKFKCSTHHKHLQ
Sbjct: 301 SKFKCSTHHKHLQ 313



>Os10g0343100 t-snare domain containing protein
          Length = 367

 Score = 89.4 bits (220), Expect = 4e-18,   Method: Compositional matrix adjust.
 Identities = 64/174 (36%), Positives = 88/174 (50%), Gaps = 16/174 (9%)

Query: 1   MAEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQ----- 55
           MA  F RWE DP FPAAE VQ+SADRME VYR  L ERK +      T            
Sbjct: 1   MATSFDRWEKDPFFPAAEEVQESADRMESVYRRWLQERKEVGGGAVETAAERGGGGWGRA 60

Query: 56  ---YERDVCTALGTTKWQLEQFEREV--NAAALSNKSNSRENAILQFRQFIRAIAEQISQ 110
                R++ TALGT KWQL++ +R +  N + +    ++R     +   F+ AI  +I +
Sbjct: 61  AGDLRRELHTALGTAKWQLDELQRAIKSNYSVVLAGKDTRA----RHDDFVSAIGHRILE 116

Query: 111 VEDSLESLRNDSIRTP-KHSYLTEHDGDELASFLS-GSNRKDNHVIYSTGTDEI 162
           VE  L+       R P     L E + +ELA FLS G+ +K + V+  T   +I
Sbjct: 117 VEKFLKESNTTEGRGPLSWVRLDEGEREELAHFLSAGTYQKRDEVVTITSAGDI 170



>Os08g0244100 t-snare domain containing protein
          Length = 361

 Score = 88.6 bits (218), Expect = 6e-18,   Method: Compositional matrix adjust.
 Identities = 62/147 (42%), Positives = 85/147 (57%), Gaps = 13/147 (8%)

Query: 2   AEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHERKLIQDDTSHTKRRVSMQYERDVC 61
           A G  +W+ D  F AAE VQ+SAD ME +YR  + ER    D  ++ +    +Q  R++ 
Sbjct: 4   AGGLEQWQKDGFFQAAEEVQESADLMESIYRTWMRER----DSGANLEELSDLQ--RELK 57

Query: 62  TALGTTKWQLEQFEREVNAAALSNKSNSRENAIL-QFRQFIRAIAEQISQVEDSLE--SL 118
           TALGT KWQLEQFER   A ++SN   S E   L + RQF+ A+ +QISQVE  +   S+
Sbjct: 58  TALGTAKWQLEQFER---AISMSNDKYSLEEGTLARRRQFVVAMEDQISQVEKQINDYSI 114

Query: 119 RNDSIRTPKHSYLTEHDGDELASFLSG 145
            ND  R      L + + D+L +FLS 
Sbjct: 115 DNDR-RGLNWVKLDDEERDDLVAFLSA 140



>Os03g0207200 t-snare domain containing protein
          Length = 364

 Score = 79.0 bits (193), Expect = 5e-15,   Method: Compositional matrix adjust.
 Identities = 56/158 (35%), Positives = 81/158 (51%), Gaps = 15/158 (9%)

Query: 1   MAEGFGRWEADPLFPAAECVQDSADRMEGVYRLLLHER--------KLIQDDTSHTKRRV 52
           MA  F RWE DP FPAAE VQ SADRME VYR+ + +R                      
Sbjct: 1   MATSFDRWEKDPFFPAAEEVQGSADRMESVYRIWVQDRSGGDSEAAAAAAAAAVGGGGLP 60

Query: 53  SMQYERDVCTALGTTKWQLEQFEREV--NAAALSNKSNSRENAILQFRQFIRAIAEQISQ 110
           + +  R++ TALGT KWQL++ ER +  N    S   +++     +   F+ AI  +I +
Sbjct: 61  AGELRRELHTALGTAKWQLDELERAIRSNDKVFSAGKDTKA----RHDDFVAAIGCRILE 116

Query: 111 VEDSL-ESLRNDSIRTPKHSYLTEHDGDELASFLSGSN 147
           VE++L ES   +         L E + ++LA+FLS S+
Sbjct: 117 VENNLKESNVAEGRGALSWIDLDEDERNDLATFLSASS 154



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.131    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,253,949
Number of extensions: 473563
Number of successful extensions: 1100
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1096
Number of HSP's successfully gapped: 4
Length of query: 335
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 234
Effective length of database: 11,762,187
Effective search space: 2752351758
Effective search space used: 2752351758
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)