BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0186100 Os05g0186100|J065011J02
         (152 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0186100  Hpt domain containing protein                       310   2e-85
Os01g0743800  Similar to Histidine-containing phosphotransfe...   185   1e-47
Os08g0557700  Similar to Histidine-containing phosphotransfe...   105   1e-23
Os09g0567400  Similar to Histidine-containing phosphotransfe...    99   2e-21

>Os05g0186100 Hpt domain containing protein
          Length = 152

 Score =  310 bits (795), Expect = 2e-85,   Method: Compositional matrix adjust.
 Identities = 152/152 (100%), Positives = 152/152 (100%)

Query: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNI 60
           MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNI
Sbjct: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNI 60

Query: 61  EQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKM 120
           EQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKM
Sbjct: 61  EQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKM 120

Query: 121 KREHATLKQKLESYFQLLRQVGPRDYAVSSRK 152
           KREHATLKQKLESYFQLLRQVGPRDYAVSSRK
Sbjct: 121 KREHATLKQKLESYFQLLRQVGPRDYAVSSRK 152



>Os01g0743800 Similar to Histidine-containing phosphotransfer protein 4
          Length = 187

 Score =  185 bits (469), Expect = 1e-47,   Method: Compositional matrix adjust.
 Identities = 89/129 (68%), Positives = 105/129 (81%), Gaps = 1/129 (0%)

Query: 20  QGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNIEQAIDKYPQDFYRLDSLVQ 79
           +GYLDEQF Q+E+LQDE+SPNFVEEV  LFFKDS RL++NIEQA++KYP+DF R D+ +Q
Sbjct: 56  KGYLDEQFCQVEDLQDEASPNFVEEVVTLFFKDSGRLMSNIEQALEKYPRDFNRWDTYMQ 115

Query: 80  QLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKMKREHATLKQKLESYFQLLR 139
           QLKGS  SSIGA RMKNEC  F+ +C   N+EGC RS QK+KREHA L+QKLESYFQLLR
Sbjct: 116 QLKGS-CSSIGASRMKNECMSFRDSCGQGNVEGCMRSFQKVKREHAVLRQKLESYFQLLR 174

Query: 140 QVGPRDYAV 148
           Q GP   A 
Sbjct: 175 QAGPAGAAT 183



>Os08g0557700 Similar to Histidine-containing phosphotransfer protein
          Length = 147

 Score =  105 bits (262), Expect = 1e-23,   Method: Compositional matrix adjust.
 Identities = 55/141 (39%), Positives = 87/141 (61%), Gaps = 1/141 (0%)

Query: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNI 60
           M  + L  Q+  +  N+F  G LD+QF QL+ LQD ++P+FV EV  LF  D  R++  +
Sbjct: 1   MAAAALTSQLNALVNNMFAMGLLDDQFQQLQMLQDSTAPDFVSEVVTLFCDDGERIICEL 60

Query: 61  EQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKM 120
            + ++K   DF R+DS V QLKGS S+S+GA ++KN C  F+  C  R+ +GC ++L  +
Sbjct: 61  SRQLEKPNVDFDRVDSYVHQLKGS-SASVGAQKVKNTCIQFREFCQQRSRDGCLKTLDLV 119

Query: 121 KREHATLKQKLESYFQLLRQV 141
           + E   L+ K ++  QL +Q+
Sbjct: 120 RTEFYDLRNKFQAMLQLEQQI 140



>Os09g0567400 Similar to Histidine-containing phosphotransfer protein
          Length = 149

 Score = 98.6 bits (244), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 53/146 (36%), Positives = 88/146 (60%), Gaps = 2/146 (1%)

Query: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDES-SPNFVEEVAALFFKDSSRLLTN 59
           M  + LR Q+  +  ++F QG +DEQF QL+ LQDE  +P FV EV  LF  D+ R++  
Sbjct: 1   MAAAALRDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINE 60

Query: 60  IEQAIDKYPQDFYRLDSLVQQLKGSGSSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQK 119
           I   +++   +F ++D+ V QLKGS S+S+GA ++K  C  F+  C D++ +GC  +L  
Sbjct: 61  IATLLEQPVVNFDKVDAYVHQLKGS-SASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAV 119

Query: 120 MKREHATLKQKLESYFQLLRQVGPRD 145
           ++ +   L+ K ++  QL +Q+   D
Sbjct: 120 VRNDFYDLRNKFQTMLQLEQQIQAYD 145



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,478,829
Number of extensions: 157327
Number of successful extensions: 498
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 495
Number of HSP's successfully gapped: 4
Length of query: 152
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 60
Effective length of database: 12,232,113
Effective search space: 733926780
Effective search space used: 733926780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)