BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0678200 Os04g0678200|AK106193
         (183 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0678200  Protein of unknown function DUF1218 family pro...   246   6e-66
Os07g0462200  Protein of unknown function DUF1218 family pro...   108   3e-24
Os09g0281300  Protein of unknown function DUF1218 family pro...    93   1e-19
Os08g0261100  Protein of unknown function DUF1218 family pro...    79   2e-15

>Os04g0678200 Protein of unknown function DUF1218 family protein
          Length = 183

 Score =  246 bits (628), Expect = 6e-66,   Method: Compositional matrix adjust.
 Identities = 123/161 (76%), Positives = 123/161 (76%)

Query: 23  GAEKRRSTATFSEDTSGRQYCVYSSDXXXXXXXXXXXXXXXXXXXXXXXTRCFCCGRALS 82
           GAEKRRSTATFSEDTSGRQYCVYSSD                       TRCFCCGRALS
Sbjct: 23  GAEKRRSTATFSEDTSGRQYCVYSSDAATGYGIGALLLLLAGQAVVMVVTRCFCCGRALS 82

Query: 83  PGRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGVXXX 142
           PGRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGV   
Sbjct: 83  PGRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGVFAA 142

Query: 143 XXXXXXXXXXXXELHYLFFAKARHAAAVPPPIVGGIGMTRM 183
                       ELHYLFFAKARHAAAVPPPIVGGIGMTRM
Sbjct: 143 GAAFTFLTALFAELHYLFFAKARHAAAVPPPIVGGIGMTRM 183



>Os07g0462200 Protein of unknown function DUF1218 family protein
          Length = 185

 Score =  108 bits (269), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 59/158 (37%), Positives = 78/158 (49%), Gaps = 1/158 (0%)

Query: 24  AEKRRSTATFSEDTSGR-QYCVYSSDXXXXXXXXXXXXXXXXXXXXXXXTRCFCCGRALS 82
           AE+RRS AT + D+     YCVY SD                       ++CFCCGR L 
Sbjct: 24  AEQRRSKATVTPDSEKLYDYCVYDSDIATGYGVGALLLLAAAQAVVMLASKCFCCGRGLK 83

Query: 83  PGRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGVXXX 142
           PG  RA +   F+  W TF+IAE CL+AGS+RNAYHT+Y  + +     C  +RKGV   
Sbjct: 84  PGGSRACALILFLFAWLTFLIAEACLMAGSIRNAYHTRYRGMFVGESVSCETVRKGVFAA 143

Query: 143 XXXXXXXXXXXXELHYLFFAKARHAAAVPPPIVGGIGM 180
                       E +Y+ ++K+R AA   P     IGM
Sbjct: 144 GAAFTFFTAILSEFYYVSYSKSRDAAGGAPYGGSNIGM 181



>Os09g0281300 Protein of unknown function DUF1218 family protein
          Length = 190

 Score = 92.8 bits (229), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 51/146 (34%), Positives = 69/146 (47%)

Query: 24  AEKRRSTATFSEDTSGRQYCVYSSDXXXXXXXXXXXXXXXXXXXXXXXTRCFCCGRALSP 83
           AE+RRST +   D+    +CVY SD                       T+C C G+ L+P
Sbjct: 29  AERRRSTGSIVTDSFNSTFCVYDSDIATGYGVGAFLFLLSGQSLLMVVTKCMCFGKPLAP 88

Query: 84  GRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGPPRCAMLRKGVXXXX 143
           G  RA+S   F   W TF+IAE CL+AG+ +NAYHT+Y  +V  G   C  LRKGV    
Sbjct: 89  GGSRAWSIIYFASSWVTFMIAESCLIAGATKNAYHTRYRHMVYVGSWTCESLRKGVFIAG 148

Query: 144 XXXXXXXXXXXELHYLFFAKARHAAA 169
                         Y+++ K+   AA
Sbjct: 149 AVFVVFTMILNVYFYMYYTKSTSQAA 174



>Os08g0261100 Protein of unknown function DUF1218 family protein
          Length = 199

 Score = 79.0 bits (193), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 69/161 (42%), Gaps = 5/161 (3%)

Query: 23  GAEKRRSTATFSEDTSG-RQYCVYSSDXXXXXXXXXXXXXXXXXXXXXXXTRCFCCGRAL 81
           GAE+RRST     D    R YC+Y +D                       TRC C G AL
Sbjct: 31  GAERRRSTGKVVPDEYDLRSYCLYDTDASTVYGVAAFFVLLLAQALVTAATRCLCLGPAL 90

Query: 82  SPGRWRAFSGFCFIVCWFTFVIAELCLLAGSVRNAYHTKYSTLVISGP-PRCAMLRKGVX 140
           S    R  +   F++ W TF+ AE CL+ GSVRNAYHTKY    +      CA LRKGV 
Sbjct: 91  SS---RGCAVASFVLSWITFLTAEACLIGGSVRNAYHTKYLGYYMKHDLVTCATLRKGVF 147

Query: 141 XXXXXXXXXXXXXXELHYLFFAKARHAAAVPPPIVGGIGMT 181
                          ++Y  +++A     +      G+ MT
Sbjct: 148 AAAAALMLINLLASLMYYWSYSRAATGGFIKHQNEVGMSMT 188



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.329    0.138    0.464 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,774,200
Number of extensions: 125880
Number of successful extensions: 328
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 323
Number of HSP's successfully gapped: 4
Length of query: 183
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 88
Effective length of database: 12,075,471
Effective search space: 1062641448
Effective search space used: 1062641448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 153 (63.5 bits)