BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0636400 Os04g0636400|AK061848
(141 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0636400 Similar to Senescence-associated protein 6 224 1e-59
Os04g0652700 Similar to Nuclease I 73 8e-14
Os01g0128100 Similar to Bifunctional nuclease (Fragment) 72 1e-13
Os01g0128200 Similar to Nuclease I 69 1e-12
>Os04g0636400 Similar to Senescence-associated protein 6
Length = 141
Score = 224 bits (572), Expect = 1e-59, Method: Compositional matrix adjust.
Identities = 111/129 (86%), Positives = 111/129 (86%)
Query: 1 MASDKCSSFXXXXXXXXXXXXXXXXXXHSWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTE 60
MASDKCSSF HSWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTE
Sbjct: 1 MASDKCSSFLLAAAAAAVLVLASAPVAHSWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTE 60
Query: 61 EADGDLSALCVWPDQVRHWYKYRWTSPLHFIDTLTKPAASSTQGIAMARMVRRICASLAR 120
EADGDLSALCVWPDQVRHWYKYRWTSPLHFIDTLTKPAASSTQGIAMARMVRRICASLAR
Sbjct: 61 EADGDLSALCVWPDQVRHWYKYRWTSPLHFIDTLTKPAASSTQGIAMARMVRRICASLAR 120
Query: 121 SRTSPLSLC 129
SRTSPLSLC
Sbjct: 121 SRTSPLSLC 129
>Os04g0652700 Similar to Nuclease I
Length = 290
Score = 72.8 bits (177), Expect = 8e-14, Method: Compositional matrix adjust.
Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 2/66 (3%)
Query: 28 HSWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTEEADGDLSALCVWPDQVRHWYKYRWTSP 87
H+W KEGH + C+IA+ L AA AV+ LL A G+L+ C W D R ++YRW+SP
Sbjct: 25 HAWGKEGHYMVCKIAEGFLTKEAATAVKELLPGWAGGELAETCSWADTER--FRYRWSSP 82
Query: 88 LHFIDT 93
LHF DT
Sbjct: 83 LHFADT 88
>Os01g0128100 Similar to Bifunctional nuclease (Fragment)
Length = 291
Score = 72.4 bits (176), Expect = 1e-13, Method: Compositional matrix adjust.
Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Query: 28 HSWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTEEADGDLSALCVWPDQVRHWYKYRWTSP 87
H+W GH++ C+IAQ L AAA AVR LL A G+LS+LC W D V+ +Y W++P
Sbjct: 26 HAWGIHGHLIVCQIAQGRLSDAAAAAVRGLLPSYAGGNLSSLCSWADGVK--LRYPWSAP 83
Query: 88 LHFIDT 93
LH+IDT
Sbjct: 84 LHYIDT 89
>Os01g0128200 Similar to Nuclease I
Length = 308
Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 30/65 (46%), Positives = 43/65 (66%), Gaps = 2/65 (3%)
Query: 29 SWSKEGHMLTCRIAQDLLEPAAAHAVRNLLTEEADGDLSALCVWPDQVRHWYKYRWTSPL 88
+W K+GH++ C+IA+ L AA AV LL E A G+LS +C W D+VR + Y W+ PL
Sbjct: 33 AWGKQGHIIVCKIAEKYLSEKAAAAVEELLPESAGGELSTVCPWADEVR--FHYYWSRPL 90
Query: 89 HFIDT 93
H+ +T
Sbjct: 91 HYANT 95
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.322 0.128 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,004,499
Number of extensions: 140600
Number of successful extensions: 389
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 385
Number of HSP's successfully gapped: 4
Length of query: 141
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 50
Effective length of database: 12,284,327
Effective search space: 614216350
Effective search space used: 614216350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 151 (62.8 bits)