BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0577700 Os04g0577700|AK108703
         (367 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0577700  Protein of unknown function DUF623, plant doma...   392   e-109
Os02g0679700  Protein of unknown function DUF623, plant doma...    98   1e-20
Os10g0532600  Protein of unknown function DUF623, plant doma...    85   9e-17
Os03g0126600                                                       70   2e-12

>Os04g0577700 Protein of unknown function DUF623, plant domain containing protein
          Length = 367

 Score =  392 bits (1007), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 200/262 (76%), Positives = 200/262 (76%)

Query: 76  VPSASSSFLWXXXXXXXXXXXXXFKRESSATTPSFSGLLRQLNELEQSVMTWGWKSPRRG 135
           VPSASSSFLW             FKRESSATTPSFSGLLRQLNELEQSVMTWGWKSPRRG
Sbjct: 76  VPSASSSFLWDDADAEADGEEVDFKRESSATTPSFSGLLRQLNELEQSVMTWGWKSPRRG 135

Query: 136 NHFSXXXXXXXXXXXXXXXVVLHRAVDAGGKRSNKEDDAKFXXXXXXXHCPTTQLHRKVK 195
           NHFS               VVLHRAVDAGGKRSNKEDDAKF       HCPTTQLHRKVK
Sbjct: 136 NHFSPPPPPPPPPPLPLRPVVLHRAVDAGGKRSNKEDDAKFSSPPPSSHCPTTQLHRKVK 195

Query: 196 SVDQRNREDDEAHFXXXXXXXXXXXXXXXRNVKGVDKGGSKEDSKHCPPPPQAPKHRKTK 255
           SVDQRNREDDEAHF               RNVKGVDKGGSKEDSKHCPPPPQAPKHRKTK
Sbjct: 196 SVDQRNREDDEAHFAPPAPPPLPLPPQQLRNVKGVDKGGSKEDSKHCPPPPQAPKHRKTK 255

Query: 256 SCDNNDGFTAGKLDGSLAVVKQSEDPRGDFRRSMLNMIVENRIVTGDXXXXXXXXXXXXN 315
           SCDNNDGFTAGKLDGSLAVVKQSEDPRGDFRRSMLNMIVENRIVTGD            N
Sbjct: 256 SCDNNDGFTAGKLDGSLAVVKQSEDPRGDFRRSMLNMIVENRIVTGDELRELLHRFLELN 315

Query: 316 APHHHDAILRAFAEIWDEVFAG 337
           APHHHDAILRAFAEIWDEVFAG
Sbjct: 316 APHHHDAILRAFAEIWDEVFAG 337



>Os02g0679700 Protein of unknown function DUF623, plant domain containing protein
          Length = 281

 Score = 97.8 bits (242), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 47/69 (68%), Positives = 51/69 (73%)

Query: 268 LDGSLAVVKQSEDPRGDFRRSMLNMIVENRIVTGDXXXXXXXXXXXXNAPHHHDAILRAF 327
           LDGS+AVVKQS+DP GDFR+SML MIVEN IV G+            NAPHHHD ILRAF
Sbjct: 168 LDGSVAVVKQSDDPLGDFRQSMLQMIVENGIVAGEDLREMLRRFLTLNAPHHHDVILRAF 227

Query: 328 AEIWDEVFA 336
           AEIWD VFA
Sbjct: 228 AEIWDGVFA 236



>Os10g0532600 Protein of unknown function DUF623, plant domain containing protein
          Length = 263

 Score = 84.7 bits (208), Expect = 9e-17,   Method: Compositional matrix adjust.
 Identities = 41/71 (57%), Positives = 48/71 (67%)

Query: 267 KLDGSLAVVKQSEDPRGDFRRSMLNMIVENRIVTGDXXXXXXXXXXXXNAPHHHDAILRA 326
           +++ S+AVVK+S DP  DFRRSML MIVE  IV G             N+PHHH  ILRA
Sbjct: 154 RVEESVAVVKESADPLFDFRRSMLQMIVEKEIVGGAELRELLHRFLPLNSPHHHHVILRA 213

Query: 327 FAEIWDEVFAG 337
           FAEIW+EVFAG
Sbjct: 214 FAEIWEEVFAG 224



>Os03g0126600 
          Length = 236

 Score = 70.5 bits (171), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 43/111 (38%), Positives = 56/111 (50%), Gaps = 2/111 (1%)

Query: 229 GVDKGGSKEDSKHCPPPPQAPKHRKTKS--CDNNDGFTAGKLDGSLAVVKQSEDPRGDFR 286
           GV   G +ED    P  P  P+HR++ S              + S+ VV +S+DP GDFR
Sbjct: 102 GVRALGVREDRGIRPATPPPPRHRRSASDASSGGRRRRGRLEEESVPVVTESDDPLGDFR 161

Query: 287 RSMLNMIVENRIVTGDXXXXXXXXXXXXNAPHHHDAILRAFAEIWDEVFAG 337
           RSM  MIVEN I                N+  HH  ILRAFA++ +E+FAG
Sbjct: 162 RSMAQMIVENEITATPELRELLHRFLSLNSSRHHHLILRAFADVCEELFAG 212



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.130    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,381,281
Number of extensions: 289368
Number of successful extensions: 1127
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 1129
Number of HSP's successfully gapped: 5
Length of query: 367
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 265
Effective length of database: 11,709,973
Effective search space: 3103142845
Effective search space used: 3103142845
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)