BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0552300 Os04g0552300|AK061100
         (217 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0552300  Negative regulatory factor PREG family protein      304   3e-83
Os10g0563900  Negative regulatory factor PREG family protein       95   5e-20
Os04g0544200  Negative regulatory factor PREG family protein       80   8e-16
Os02g0652000  PREG-like protein                                    74   8e-14

>Os04g0552300 Negative regulatory factor PREG family protein
          Length = 217

 Score =  304 bits (779), Expect = 3e-83,   Method: Compositional matrix adjust.
 Identities = 158/203 (77%), Positives = 158/203 (77%)

Query: 1   MASTELASDVYALPCGDDGTTALSTPVVVSVLASLLERHIARNXXXXXXXXXXXXXXXXX 60
           MASTELASDVYALPCGDDGTTALSTPVVVSVLASLLERHIARN                 
Sbjct: 1   MASTELASDVYALPCGDDGTTALSTPVVVSVLASLLERHIARNERDQAAAADGEAARRAR 60

Query: 61  XFDSGTVLDMSLHAFLERFSRYANVSPQVYVVAYAYXXXXXXXXXXXXXSANAQRLLTTA 120
            FDSGTVLDMSLHAFLERFSRYANVSPQVYVVAYAY             SANAQRLLTTA
Sbjct: 61  AFDSGTVLDMSLHAFLERFSRYANVSPQVYVVAYAYLDRLRRGDGVRVVSANAQRLLTTA 120

Query: 121 ILVASKFVEDRNYKNSYFAAVGGLTXXXXXXXXXXXXXXMQFRLNVSVSVFQSYCRHLER 180
           ILVASKFVEDRNYKNSYFAAVGGLT              MQFRLNVSVSVFQSYCRHLER
Sbjct: 121 ILVASKFVEDRNYKNSYFAAVGGLTAAELSSLELDFLFLMQFRLNVSVSVFQSYCRHLER 180

Query: 181 EVSYGGGYQVERCLKKALVCSGE 203
           EVSYGGGYQVERCLKKALVCSGE
Sbjct: 181 EVSYGGGYQVERCLKKALVCSGE 203



>Os10g0563900 Negative regulatory factor PREG family protein
          Length = 212

 Score = 94.7 bits (234), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 60/167 (35%), Positives = 81/167 (48%), Gaps = 1/167 (0%)

Query: 19  GTTALSTPVVVSVLASLLERHIARNXXXXXXXXXXXXXXXXXXFDSGTVLDMSLHAFLER 78
           G  A + P VV++L+SLL+R   RN                  F   T   +S+  +LER
Sbjct: 4   GEVAEAVPRVVAILSSLLQRVAERNDAAAAAAAVGEEAAAVSAFQGLTKPAISIGGYLER 63

Query: 79  FSRYANVSPQVYVVAYAYXXX-XXXXXXXXXXSANAQRLLTTAILVASKFVEDRNYKNSY 137
             R+AN SP  YVVAY Y              S N  RLL T++L A KFV+D  Y N+Y
Sbjct: 64  IFRFANCSPSCYVVAYIYLDRFLRRRPALAVDSFNVHRLLITSVLTAVKFVDDICYNNAY 123

Query: 138 FAAVGGLTXXXXXXXXXXXXXXMQFRLNVSVSVFQSYCRHLEREVSY 184
           FA VGG++              + F LNV+ + F SYC  L+ E++Y
Sbjct: 124 FARVGGISLMEMNYLEVDFLFGIAFDLNVTPAAFASYCAVLQSEMTY 170



>Os04g0544200 Negative regulatory factor PREG family protein
          Length = 212

 Score = 80.5 bits (197), Expect = 8e-16,   Method: Compositional matrix adjust.
 Identities = 56/162 (34%), Positives = 75/162 (46%), Gaps = 5/162 (3%)

Query: 26  PVVVSVLASLLERHIARN-XXXXXXXXXXXXXXXXXXFDSGTVLDMSLHAFLERFSRYAN 84
           P VV VLA+LLER   RN                   F + T  D+++ A++ R +R+A 
Sbjct: 11  PRVVGVLAALLERVTERNDAAAAELELAVAGAPAASAFRATTKPDITVRAYMARIARFAG 70

Query: 85  VSPQVYVVAYAYX----XXXXXXXXXXXXSANAQRLLTTAILVASKFVEDRNYKNSYFAA 140
            SP  YVVAY Y                 S +  RLL TA+L A KF++D  Y N+YFA 
Sbjct: 71  CSPACYVVAYIYLDRLLRRRRRACAFSVDSYSVHRLLITAVLAAVKFMDDICYNNAYFAK 130

Query: 141 VGGLTXXXXXXXXXXXXXXMQFRLNVSVSVFQSYCRHLEREV 182
           VGG++              + F LNVS   F  YC  L+ E+
Sbjct: 131 VGGVSLPEMNYLEVDFLFGVGFDLNVSPETFGHYCAVLQSEM 172



>Os02g0652000 PREG-like protein
          Length = 212

 Score = 73.9 bits (180), Expect = 8e-14,   Method: Compositional matrix adjust.
 Identities = 53/163 (32%), Positives = 71/163 (43%), Gaps = 8/163 (4%)

Query: 26  PVVVSVLASLLERHIARNXXXXXXXXXXXXXXXXXXFDSGTVLDMSLHAFLERFSRYANV 85
           P VV+ LA +LER   RN                  F +     +S+ A+  R +R+A  
Sbjct: 17  PRVVAALAGILERVAGRNDAAATPAELAAAPASP--FRATAKPGISVRAYAARIARFAGC 74

Query: 86  SPQVYVVAYAYXXXXXXXXXXXXXSANA------QRLLTTAILVASKFVEDRNYKNSYFA 139
           SP  YVVAY Y              A A       RLL TA+L A KF++D  Y N+YFA
Sbjct: 75  SPACYVVAYIYLDRLLRRGRRCLALALAVDSYSVHRLLITAVLSAVKFMDDICYNNAYFA 134

Query: 140 AVGGLTXXXXXXXXXXXXXXMQFRLNVSVSVFQSYCRHLEREV 182
            VGG++              + F LNV+   F  YC  L+ E+
Sbjct: 135 KVGGISLAEMNYLEVDFLFGVGFDLNVTPETFADYCAVLQSEM 177



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.132    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,656,445
Number of extensions: 107980
Number of successful extensions: 211
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 4
Length of query: 217
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 120
Effective length of database: 11,971,043
Effective search space: 1436525160
Effective search space used: 1436525160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 154 (63.9 bits)