BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0400600 Os04g0400600|Os04g0400600
         (225 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0400600  Heavy metal transport/detoxification protein d...   166   2e-41
Os04g0400700                                                      125   2e-29
Os04g0400800  Heavy metal transport/detoxification protein d...    70   9e-13
Os04g0400000  Heavy metal transport/detoxification protein d...    65   4e-11
Os04g0400500  Heavy metal transport/detoxification protein d...    65   5e-11
Os04g0401000  Heavy metal transport/detoxification protein d...    64   8e-11

>Os04g0400600 Heavy metal transport/detoxification protein domain containing
           protein
          Length = 225

 Score =  166 bits (419), Expect = 2e-41,   Method: Compositional matrix adjust.
 Identities = 89/145 (61%), Positives = 89/145 (61%)

Query: 1   MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRGSFCAEELRTCIWRK 60
           MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRGSFCAEELRTCIWRK
Sbjct: 1   MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRGSFCAEELRTCIWRK 60

Query: 61  AGCKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFIMVEREHCNKQ 120
           AGCK                                            FIMVEREHCNKQ
Sbjct: 61  AGCKIIVSIVIVEVWPPPPPAVTVNVKADVTATAVAAAEAAAKATAEAFIMVEREHCNKQ 120

Query: 121 PPACKMXXXXXXXXXXXXCNKTATD 145
           PPACKM            CNKTATD
Sbjct: 121 PPACKMVPFPYPVPYPVPCNKTATD 145



>Os04g0400700 
          Length = 247

 Score =  125 bits (314), Expect = 2e-29,   Method: Compositional matrix adjust.
 Identities = 62/64 (96%), Positives = 62/64 (96%)

Query: 1  MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRGSFCAEELRTCIWRK 60
          MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRG FCAEELR CIWRK
Sbjct: 1  MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYEGEKVIVRGGFCAEELRKCIWRK 60

Query: 61 AGCK 64
          AGCK
Sbjct: 61 AGCK 64



>Os04g0400800 Heavy metal transport/detoxification protein domain containing
          protein
          Length = 282

 Score = 70.5 bits (171), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 37/64 (57%), Positives = 49/64 (76%), Gaps = 2/64 (3%)

Query: 1  MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYE--GEKVIVRGSFCAEELRTCIW 58
          M ILVI+ +++CCRCKEK+ KIL  LR K+ IEK EYE   ++VIVRG+F  ++LR+ IW
Sbjct: 1  MGILVISADLKCCRCKEKLSKILCSLRDKYGIEKTEYEDKDDRVIVRGNFPTDKLRSVIW 60

Query: 59 RKAG 62
           KAG
Sbjct: 61 CKAG 64



>Os04g0400000 Heavy metal transport/detoxification protein domain containing
          protein
          Length = 215

 Score = 65.1 bits (157), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 37/65 (56%), Positives = 49/65 (75%), Gaps = 2/65 (3%)

Query: 2  AILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYE--GEKVIVRGSFCAEELRTCIWR 59
          +ILVITV+++CCRC+ KI K+L+CL+ ++CIEK E+E   +KVIVRG F A  LR  I  
Sbjct: 4  SILVITVDLKCCRCRAKITKVLDCLKEEYCIEKTEFEEKDDKVIVRGKFDAGSLRNKICC 63

Query: 60 KAGCK 64
          KAG K
Sbjct: 64 KAGGK 68



>Os04g0400500 Heavy metal transport/detoxification protein domain containing
          protein
          Length = 213

 Score = 64.7 bits (156), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 37/65 (56%), Positives = 49/65 (75%), Gaps = 2/65 (3%)

Query: 2  AILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYE--GEKVIVRGSFCAEELRTCIWR 59
          +ILVITV+++CCRC+ KI K+L+CL+ ++CIEK E+E   +KVIVRG F A  LR  I  
Sbjct: 10 SILVITVDLKCCRCRAKITKVLDCLKAEYCIEKTEFEEKDDKVIVRGKFDAGCLRNKICC 69

Query: 60 KAGCK 64
          KAG K
Sbjct: 70 KAGGK 74



>Os04g0401000 Heavy metal transport/detoxification protein domain containing
          protein
          Length = 210

 Score = 63.9 bits (154), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 36/64 (56%), Positives = 45/64 (70%), Gaps = 2/64 (3%)

Query: 1  MAILVITVNMQCCRCKEKIDKILNCLRCKHCIEKIEYE--GEKVIVRGSFCAEELRTCIW 58
          M ILVI V++QCCRC  KI K+L CL  ++CIEK+EY+    +VIVRG F  E+L   IW
Sbjct: 1  MGILVILVDLQCCRCDAKIRKVLGCLEEEYCIEKVEYDVKNNRVIVRGKFDPEKLCKKIW 60

Query: 59 RKAG 62
           KAG
Sbjct: 61 CKAG 64



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.138    0.468 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,432,079
Number of extensions: 73570
Number of successful extensions: 293
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 287
Number of HSP's successfully gapped: 6
Length of query: 225
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 128
Effective length of database: 11,971,043
Effective search space: 1532293504
Effective search space used: 1532293504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 154 (63.9 bits)