BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0316200 Os03g0316200|AK071812
         (341 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0316200  Similar to Galactinol synthase (Fragment)           611   e-175
Os07g0687900  WSI76 protein induced by water stress               504   e-143
Os01g0880200  Glycosyl transferase, family 8 protein               86   3e-17
Os03g0184300  Glycosyl transferase, family 8 protein               80   2e-15

>Os03g0316200 Similar to Galactinol synthase (Fragment)
          Length = 341

 Score =  611 bits (1575), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 298/341 (87%), Positives = 298/341 (87%)

Query: 1   MAPPQLXXXXXXXXXXXXXXXXXXYVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVL 60
           MAPPQL                  YVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVL
Sbjct: 1   MAPPQLAGKMTAKAAAAVKPATRAYVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVL 60

Query: 61  PDVPESHRRILISQGCIVREIEPVYPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLD 120
           PDVPESHRRILISQGCIVREIEPVYPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLD
Sbjct: 61  PDVPESHRRILISQGCIVREIEPVYPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLD 120

Query: 121 ADIQVFDNIDELFELPKGHFYAVMDCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPP 180
           ADIQVFDNIDELFELPKGHFYAVMDCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPP
Sbjct: 121 ADIQVFDNIDELFELPKGHFYAVMDCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPP 180

Query: 181 PALYFNAGMFVHEPSMATAKSLLDTLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLA 240
           PALYFNAGMFVHEPSMATAKSLLDTLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLA
Sbjct: 181 PALYFNAGMFVHEPSMATAKSLLDTLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLA 240

Query: 241 MLWRHPENVQLEKVKVVHYCAAGSKPWRYTGKEANMDREDIKMLVKKWWDVYNDGSLDFK 300
           MLWRHPENVQLEKVKVVHYCAAGSKPWRYTGKEANMDREDIKMLVKKWWDVYNDGSLDFK
Sbjct: 241 MLWRHPENVQLEKVKVVHYCAAGSKPWRYTGKEANMDREDIKMLVKKWWDVYNDGSLDFK 300

Query: 301 GLPPIXXXXXXXXXXXXXXXXXXXXXXXXXTVKYVTAPSAA 341
           GLPPI                         TVKYVTAPSAA
Sbjct: 301 GLPPIAAADDADEVEAAAKKPLRAALAEARTVKYVTAPSAA 341



>Os07g0687900 WSI76 protein induced by water stress
          Length = 328

 Score =  504 bits (1299), Expect = e-143,   Method: Compositional matrix adjust.
 Identities = 236/276 (85%), Positives = 252/276 (91%)

Query: 25  YVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVLPDVPESHRRILISQGCIVREIEPV 84
           YVTFLAGDGDYWKGVVGLAKGLR+V SAYPLVVAVLPDVP  HRR L+ QGC+VREI+PV
Sbjct: 21  YVTFLAGDGDYWKGVVGLAKGLRRVRSAYPLVVAVLPDVPGEHRRKLVEQGCVVREIQPV 80

Query: 85  YPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLDADIQVFDNIDELFELPKGHFYAVM 144
           YPPE+QTQFAMAYYVINYSKLRIWEFVEYERMVYLDADIQVFDNID LF+L KG FYAV 
Sbjct: 81  YPPESQTQFAMAYYVINYSKLRIWEFVEYERMVYLDADIQVFDNIDHLFDLDKGAFYAVK 140

Query: 145 DCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPPPALYFNAGMFVHEPSMATAKSLLD 204
           DCFCEKTWSHTPQY IGYCQQ PD+VAWP  ELGPPP LYFNAGMFVHEP + TAK LLD
Sbjct: 141 DCFCEKTWSHTPQYDIGYCQQRPDEVAWPERELGPPPPLYFNAGMFVHEPGLGTAKDLLD 200

Query: 205 TLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLAMLWRHPENVQLEKVKVVHYCAAGS 264
            L VT PTPFAEQDFLNMFFREQYKPIP +YNLVLAMLWRHPENV L++VKVVHYCAAGS
Sbjct: 201 ALVVTPPTPFAEQDFLNMFFREQYKPIPNVYNLVLAMLWRHPENVDLDQVKVVHYCAAGS 260

Query: 265 KPWRYTGKEANMDREDIKMLVKKWWDVYNDGSLDFK 300
           KPWR+TGKE NM+REDIKMLVK+WWD+YND SLD+K
Sbjct: 261 KPWRFTGKEENMNREDIKMLVKRWWDIYNDESLDYK 296



>Os01g0880200 Glycosyl transferase, family 8 protein
          Length = 635

 Score = 86.3 bits (212), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 66/253 (26%), Positives = 108/253 (42%), Gaps = 53/253 (20%)

Query: 25  YVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVLPDVPESHRRILISQGCIVREIEPV 84
           Y T L    +Y  G +  A+ +R+ GS    V+ V   +   HR+ L + G  VR I+ +
Sbjct: 308 YATILHSASEYVCGAITAAQSIRQAGSTRDFVILVDETISNHHRKGLEAAGWKVRIIQRI 367

Query: 85  YPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLDADIQVFDNIDELFELPKGHFYAVM 144
             P+ +     AY   NYSK R+W+  +Y++++++DAD+ +  N+D LF +P+       
Sbjct: 368 RNPKAERD---AYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNVDFLFAMPE------- 417

Query: 145 DCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPPPALYFNAGMFVHEPSMATAKSLLD 204
                                            G    L FN+G+ V EPS  T + L+D
Sbjct: 418 -----------------------------ITATGNNATL-FNSGVMVIEPSNCTFQLLMD 447

Query: 205 TLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLAMLWRHPENVQLEKVK--------- 255
            +   T     +Q +LN  F   ++ IP   N +        E V+++K +         
Sbjct: 448 HINEITSYNGGDQGYLNEIFTWWHR-IPKHMNFLKHFWEGDEEEVKVKKTRLFGADPPIL 506

Query: 256 -VVHYCAAGSKPW 267
            V+HY   G KPW
Sbjct: 507 YVLHYL--GLKPW 517



>Os03g0184300 Glycosyl transferase, family 8 protein
          Length = 500

 Score = 80.1 bits (196), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 74/286 (25%), Positives = 117/286 (40%), Gaps = 60/286 (20%)

Query: 25  YVTFLAGDGDYWKGVVGLAKGLRKVGSAYPLVVAVLPDVPESHRRILISQGCIVREIEPV 84
           Y T L     Y  G + LA+ +R+ GS   LV+     V +     L++ G   R+I+ +
Sbjct: 212 YATVLHSSDTYLCGAIVLAQSIRRAGSTRDLVLLHDHTVSKPALAALVAAGWTPRKIKRI 271

Query: 85  YPPENQTQFAMAYYVINYSKLRIWEFVEYERMVYLDADIQVFDNIDELFELPKGHFYAVM 144
             P  +      Y   NYSK R+W+  +Y+R+V++DADI V  ++D LF  P+       
Sbjct: 272 RNPRAERG---TYNEYNYSKFRLWQLTDYDRVVFVDADILVLRDLDALFGFPQ------- 321

Query: 145 DCFCEKTWSHTPQYQIGYCQQCPDKVAWPTAELGPPPALYFNAGMFVHEPSMATAKSLLD 204
                                           +G   +L FN+G+ V EPS  T +SL+ 
Sbjct: 322 -----------------------------LTAVGNDGSL-FNSGVMVIEPSQCTFQSLIR 351

Query: 205 TLRVTTPTPFAEQDFLNMFFREQYKPIPLIYNLVLAMLWRHPENVQLEKVKV-------- 256
             R        +Q FLN  F   ++ +P   N  L   W +    +  K ++        
Sbjct: 352 QRRTIRSYNGGDQGFLNEVFVWWHR-LPRRVNY-LKNFWANTTAERALKERLFRADPAEV 409

Query: 257 --VHYCAAGSKPWR-YTGKEANMDREDIKMLVK-----KWWDVYND 294
             +HY   G KPW  Y   + N +  D ++        +WW VY+D
Sbjct: 410 WSIHYL--GLKPWTCYRDYDCNWNIGDQRVYASDAAHARWWQVYDD 453



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.139    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,553,278
Number of extensions: 478811
Number of successful extensions: 891
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 892
Number of HSP's successfully gapped: 4
Length of query: 341
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 239
Effective length of database: 11,709,973
Effective search space: 2798683547
Effective search space used: 2798683547
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 156 (64.7 bits)