BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0314700 Os03g0314700|AK069184
         (224 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0314700  Nitrogen-fixing NifU, C-terminal domain contai...   387   e-108
Os11g0181500  Similar to Nitrogen fixation like protein           112   2e-25
Os12g0176200  Similar to Nitrogen fixation like protein           103   9e-23
Os06g0694500  Similar to Nitrogen fixation like protein           103   1e-22

>Os03g0314700 Nitrogen-fixing NifU, C-terminal domain containing protein
          Length = 224

 Score =  387 bits (995), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 197/224 (87%), Positives = 197/224 (87%)

Query: 1   MEASLTVXXXXXXXLSLRPQIRLRITQATPLMPPRRLQSGPSKIQTSXXXXXXXXXXXXX 60
           MEASLTV       LSLRPQIRLRITQATPLMPPRRLQSGPSKIQTS             
Sbjct: 1   MEASLTVAGAGAAALSLRPQIRLRITQATPLMPPRRLQSGPSKIQTSGARAHLAAAPAST 60

Query: 61  XXXXXXXLYSAETYELTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSC 120
                  LYSAETYELTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSC
Sbjct: 61  PPAAGGGLYSAETYELTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSC 120

Query: 121 PSSTTTMKMGIERVLKEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANYGG 180
           PSSTTTMKMGIERVLKEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANYGG
Sbjct: 121 PSSTTTMKMGIERVLKEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANYGG 180

Query: 181 SVEVVAVDGEDCLVRYEGPESIGSGIKAAIKEKFPDITNVVFLQ 224
           SVEVVAVDGEDCLVRYEGPESIGSGIKAAIKEKFPDITNVVFLQ
Sbjct: 181 SVEVVAVDGEDCLVRYEGPESIGSGIKAAIKEKFPDITNVVFLQ 224



>Os11g0181500 Similar to Nitrogen fixation like protein
          Length = 228

 Score =  112 bits (280), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 66/153 (43%), Positives = 93/153 (60%), Gaps = 7/153 (4%)

Query: 76  LTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSCPSSTTTMKMGIERVL 135
           LTAENV+ VLD+VRPYL+ADGG+V +  ++  V+ LKL+GACGSCP+S TTMKMGIER L
Sbjct: 77  LTAENVEIVLDEVRPYLMADGGNVALHEIDGNVVRLKLQGACGSCPASVTTMKMGIERRL 136

Query: 136 KEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANYGGS-VEVVAVDGEDCLV 194
            EK  + V    +   D++   E   + +   LD +RP ++  GG  +E VA++     V
Sbjct: 137 MEKIPEIVAV--EPIADEETGLELNQENIEKVLDEIRPYLSGTGGGELEFVAIEEPIVKV 194

Query: 195 RYEGPESIGSGIKAA----IKEKFPDITNVVFL 223
           R  GP +    ++ A    ++EK P I  V  L
Sbjct: 195 RLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 227



>Os12g0176200 Similar to Nitrogen fixation like protein
          Length = 226

 Score =  103 bits (257), Expect = 9e-23,   Method: Compositional matrix adjust.
 Identities = 66/159 (41%), Positives = 89/159 (55%), Gaps = 7/159 (4%)

Query: 70  SAETYELTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSCPSSTTTMKM 129
           +A    LTA NV+ VLD VRPYL ADGGDV +  +   V+ LKL+GACGSCPSS  T+K 
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKR 128

Query: 130 GIERVLKEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANY-GGSVEVVAVD 188
           GIER L EK  D V  +  V D +    E   + V   L+ +RP +A   GG ++ + + 
Sbjct: 129 GIERRLMEKIPD-VAAVEPVTDKETG-LELNEENVEKVLNEIRPYLAGTGGGGLQFLMIK 186

Query: 189 GEDCLVRYEGPESIGSGIKAAI----KEKFPDITNVVFL 223
           G    VR  GP ++   ++ A+    +EK P I  V  L
Sbjct: 187 GPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSIQIVQLL 225



>Os06g0694500 Similar to Nitrogen fixation like protein
          Length = 219

 Score =  103 bits (256), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 62/157 (39%), Positives = 93/157 (59%), Gaps = 7/157 (4%)

Query: 69  YSAETYELTAENVDRVLDDVRPYLIADGGDVTVASVEDGVISLKLEGACGSCPSSTTTMK 128
           ++     LT ENV+ VLD VRP L+ADGG+V +  ++  V+ LKL+GACGSCPSST T+K
Sbjct: 61  WAVRVLPLTEENVEMVLDQVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSTMTLK 120

Query: 129 MGIERVLKEKFGDAVKDIRQVFDDDQQPAEPTPQAVNGHLDILRPAIANY-GGSVEVVAV 187
           MGIE  L++K  + +  + Q+ D +    E     V+  LD +RP ++   GGS+++V +
Sbjct: 121 MGIETRLRDKIPEILA-VEQIVDTETG-LELNHDNVDKVLDEIRPYLSGTGGGSLDLVQI 178

Query: 188 DGEDCLVRYEGPESIGSGIKAAI----KEKFPDITNV 220
           D     VR  GP +    ++ A+    +EK P I  V
Sbjct: 179 DESVVKVRLTGPAAGVMTVRVAVTQKLREKIPSILAV 215



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.134    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,848,130
Number of extensions: 265547
Number of successful extensions: 686
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 677
Number of HSP's successfully gapped: 4
Length of query: 224
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 127
Effective length of database: 11,971,043
Effective search space: 1520322461
Effective search space used: 1520322461
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 154 (63.9 bits)