BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0230300 Os02g0230300|AY568677
         (219 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0230300  Proline-and threonine-rich protein                  309   9e-85
Os06g0553200  Conserved hypothetical protein                      127   9e-30
Os01g0726700  Conserved hypothetical protein                      103   1e-22
Os01g0201600  Virulence factor, pectin lyase fold family pro...    69   4e-12

>Os02g0230300 Proline-and threonine-rich protein
          Length = 219

 Score =  309 bits (792), Expect = 9e-85,   Method: Compositional matrix adjust.
 Identities = 156/191 (81%), Positives = 156/191 (81%)

Query: 29  EQKTYSSTTQYGGSPTPSYGSDGGYKPTPTPTPAXXXXXXXXXXXXXXXXXXXXXXXXXX 88
           EQKTYSSTTQYGGSPTPSYGSDGGYKPTPTPTPA                          
Sbjct: 29  EQKTYSSTTQYGGSPTPSYGSDGGYKPTPTPTPAYGSTPTPSYGTTPTPSYGTTPTPSYG 88

Query: 89  XXXXXXXXXDVPEVPTKHDFCGSCDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKL 148
                    DVPEVPTKHDFCGSCDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKL
Sbjct: 89  TTPSTPSTPDVPEVPTKHDFCGSCDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKL 148

Query: 149 ENLHDALSNTGTDGTGALLREGAAAYLNSIVNKKFPFTTQQVKDCIVVAMTSDGAASSQA 208
           ENLHDALSNTGTDGTGALLREGAAAYLNSIVNKKFPFTTQQVKDCIVVAMTSDGAASSQA
Sbjct: 149 ENLHDALSNTGTDGTGALLREGAAAYLNSIVNKKFPFTTQQVKDCIVVAMTSDGAASSQA 208

Query: 209 EIFKKANDYHY 219
           EIFKKANDYHY
Sbjct: 209 EIFKKANDYHY 219



>Os06g0553200 Conserved hypothetical protein
          Length = 186

 Score =  127 bits (318), Expect = 9e-30,   Method: Compositional matrix adjust.
 Identities = 64/111 (57%), Positives = 77/111 (69%), Gaps = 3/111 (2%)

Query: 108 FCGSCDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKLENLHDALSNTGTDGTGALL 167
           F GSCD+WK+HP+ IIS IGSLG I  + G  CS   G KL+ L DAL +T  D  G LL
Sbjct: 70  FTGSCDFWKSHPEKIISCIGSLGSIVGSFGDVCSGFFGSKLQTLQDALCSTRKDCYGDLL 129

Query: 168 REGAAAYLNSIVNK---KFPFTTQQVKDCIVVAMTSDGAASSQAEIFKKAN 215
           REGAAAY+NS+  K   KF +TTQQVK CI++ +TS  AA  QA +FKKAN
Sbjct: 130 REGAAAYINSVAAKKQAKFAYTTQQVKSCILLGLTSKAAAIEQAALFKKAN 180



>Os01g0726700 Conserved hypothetical protein
          Length = 662

 Score =  103 bits (256), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 51/109 (46%), Positives = 68/109 (62%), Gaps = 3/109 (2%)

Query: 112 CDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKLENLHDALSNTGTDGTGALLREGA 171
           C YW +HP VI    G    + +  G   +   G  L  + +AL+NT  DG G L REG 
Sbjct: 552 CSYWLSHPGVIWGLFGFWCPMARLFGPTAAAPFGHDL-TVPEALANTRADGVGELYREGT 610

Query: 172 AAYLNSIVNKKFPFTTQQVKDCIVVAMTSDG--AASSQAEIFKKANDYH 218
           A+ LNS+VN +FPFTTQQVKD    A++S G  AA++QA++FKKAN+ H
Sbjct: 611 ASLLNSMVNSRFPFTTQQVKDAFSAALSSGGDHAAAAQAQLFKKANEGH 659



>Os01g0201600 Virulence factor, pectin lyase fold family protein
          Length = 301

 Score = 68.6 bits (166), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 33/82 (40%), Positives = 46/82 (56%), Gaps = 1/82 (1%)

Query: 110 GSCDYWKNHPDVIISAIGSLGDIGKTLGTACSLITGKKLENLHDALSNTGTDGTGALLRE 169
           G+CDYW++HP  + SA+G                      ++ DAL+NT  DG G L+RE
Sbjct: 190 GTCDYWRSHPMEMWSALGRW-PSSVGHFFGSGSGGAGTGMSIQDALANTRGDGAGELMRE 248

Query: 170 GAAAYLNSIVNKKFPFTTQQVK 191
           GAAA LNS+    FP+T +QV+
Sbjct: 249 GAAALLNSMTRSGFPYTAEQVR 270



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.130    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,437,451
Number of extensions: 239743
Number of successful extensions: 710
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 700
Number of HSP's successfully gapped: 4
Length of query: 219
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 122
Effective length of database: 11,971,043
Effective search space: 1460467246
Effective search space used: 1460467246
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 154 (63.9 bits)